Abstract: 【Objective】 The objective of the project is to establish the stable and effective regeneration system of leavesin -vitro of Korla Fragrant Pear seedlings. 【Method】 The leaves of sterile seedlings of Korla fragrant pear cultured in vitro for about 30 days were used as the basic medium for 1/2 MS. The effects of different concentration of growth regulators on callus formation rate and adventitious bud formation rate during adventitious bud induction were studied by using TDZ (1.0 mg/L), IBA (0.3-0.9 mg/L) or NAA (0.3-0.9 mg/L) to design the formula separately to explore the effects of different concentration combinations of growth regulators on callus formation and adventitious bud formation during adventitious bud induction process. The optimal medium for adventitious bud regeneration was obtained by selecting the optimal formula and adding AgNo₃ with different mass concentration. The ratio of rooting growth regulators was designed with IBA and NAA respectively. According to the rooting rate, the average rooting number and the average rooting length, the ratio of the most suitable growth regulator were screened and the culture system of rooting was established. 【Result】 The results indicated that the optimal medium for adventitious shoot induction was 1/2 MS supplemented with 1.0 mg/L TDZ, 0.5 mg/L IBA and 0.5 mg/L AgNO₃, the frequency of callus formation and adventitious shoot regeneration were 100 % and 84.92%, respectively. The appropriate rooting induction medium was 1/2 MS + 0.5 mg/L IBA + 0.9 mg/L NAA, the rooting rate could reach 100% after 7 days dark culture. The average rooting number of each stem was 5.66, and average rooting length was 2.8 cm. 【Conclusion】 The regeneration system of leaves in -vitro of Korla Fragrant Pear seedlings was established, which laid foundation for genetic transformation of pears.