Abstract: 【Objective】 The objective of this study is to construct gRNA expression vector of eIF4E gene in melon by using CRISPR-Cas9 plant gene editing system. 【Method】 The gRNA primer was designed in the first exon region of eIF4E gene from "Queen" of melon in Xinjiang. The sgRNA cloning box was amplified by using the vector pP1C.4 as a template, the pP1C.4 vector was cut by EcoRI and XbaI enzymes, and the recombinant vector pP1C.4-eIF4E was constructed using DNA recombinase. 【Result】 The results of colony PCR detection and sequencing showed that gRNA had been successfully linked into plant gene knockout vector pP1C.4. 【Conclusion】 The plant gene knockout vector pP1C.4-eIF4E is constructed successfully and eIF4E can be directionally edited by pP1C.4-eIF4E, which is of great significance for further study on the function of eIF4E gene.