Abstract: 【Objective】 The effects of different ratios of loss-control urea and common urea on cotton growth, nitrogen uptake and yield were studied through field plot experiments. 【Method】 Eight treatments were designed to research the effects of loss-control urea and common urea on accumulation rate for plant dry matters, nitrogen uptake and yield of cotton,they were CK (N 0 kg/hm²), conventional urea (N 225 kg/hm²), loss-control urea (N 225 kg/hm²), 80% loss-control urea (N 180 kg/hm²), 70% loss-control urea (N 157.5 kg/hm²), 70% loss-control urea + 30% conventional urea (total of N 225 kg/hm²), 50% loss-control urea + 50% conventional urea (total of N 225 kg/hm²), 30% loss-control urea + 70% conventional urea (total of N 225 kg/hm²). 【Result】 The results showed that compared to the loss-control urea and common urea treatments, the application of 70% loss-control urea + 30% common urea could significantly increase the plant dry matters and yield of cotton by 14.34%, increase fertilizer nitrogen use efficiency by 10.2 percentage point; the treatments of 80% loss-control urea and common urea did nearly the same to the plant dry matters and yield of cotton, compared with the common urea, the treatment of 70% loss-control urea could significantly reduce the yield of cotton by 5.85%; the treatment of 80% loss-control urea could significantly increase the fertilizer nitrogen use efficiency of cotton by 11.26 percentage point. 【Conclusion】 The application of loss-control urea combined with conventional urea increased the utilization rate of nitrogen fertilizer in cotton, and the 7∶3 ratio of loss-control urea and conventional urea was the best.