Abstract: 【Objective】 To provide strong support for the gene sources of cotton wilt resistance molecular breeding. 【Method】 Based on the previous transcriptome sequencing and disease resistance expression data, the Fusarium wilt related genes (accession number CD486053) were screened from cotton EST database. A homologous gene named GbPR10 gene was cloned from Fusarium wilt resistant in Gossypium barbadense "06-146" by NCBI search for PR10 gene (accession number AY588276) and primer design. Bioinformatics gene expression analysis was carried out under the treatment of Fusarium oxysporum f.sp, ethylene, and salicylic acid. 【Result】 GbPR10 gene had the ORF sequence of 480 bp and encoded 159 amino acids and the Bet-v1 domain of PR10 protein and the modified glycine ring P-Loopus (GXGGXG) were found in the protein sequence. The homologous alignment of protein sequence indicated that the protein was highly consistent with other biological PR10 protein plants. Subcellular localization analyses pinpointed that GbPR10 was distributed in cytoplasm. QRT-PCR analysis showed that the expression of GbPR10 gene in different tissues of different resistant island cotton varieties was uneven and higher than that of susceptible island cotton varieties. In the roots of one pair of resistance / susceptible island cotton treated with ethylene and salicylic acid, the resistant varieties showed a tendency of up-regulation first and then down-regulation. The expression of resistant varieties was almost higher than that of susceptible varieties at later stage. 【Conclusion】 It can be concluded in this study that GbPR10 gene plays a significant role in the signal pathway of resistance to Furasium oxysporum f.sp in Gossypium barbadense.