Abstract: 【Objective】 In order to compare the resistance of staphylococcus in the nasal cavity and rectum of pigs with different growth periods in a pig farm in Yanqi, Xinjiang. 【Method】The susceptibility test of 11 antimicrobial agents to staphylococcus isolated from different parts was carried out by broth microdilution method.【Result】Result: 332 strains of rectal bacteria (83%, 332/400) and 137 strains of nasal bacteria (68.5%, 137/200) were isolated. The resistance rate of 2 month piglet rectal bacteria to tetracycline, oxacillin, ofloxacin, rifampicin and penicillin was higher than that of the nasal bacteria; the resistance rate of nasal bacteria to florfenicol was significantly higher than that of the rectal bacteria (P < 0.01). The resistance rates to clindamycin, rifampicin and amoxicillin/clavulanic acid in pregnancy were remarkably higher than those in nasal bacteria (P <0.01). The resistance rate of nasal bacteria to tetracycline, oxacillin and ceftifura was significantly higher than that of rectal bacteria (P< 0.01), and the resistance rate to penicillin was significantly higher than that of rectal bacteria (P< 0.05). The resistance rates of rectal piglets to gentamicin, clindamycin, oxacillin and ceftiofur were significantly higher than that of the pregnant pigs (P <0.05). The resistance to rifampicin and amoxicillin / clavulanic acid was only slightly higher than that of piglet rectal bacteria. The drug resistance rate of the piglet nasal bacteria to tetracycline, clindamycin and florfenicol was significantly higher than that of the pregnant porcine nasal bacteria (P< 0.01). Pregnancy swine bacteria resistant to ceftiofur was significantly higher than pup source bacteria (P< 0.01). All the isolates were sensitive to amikacin.【Conclusion】The drug resistance of staphylococcus isolated from the pig farm is serious and the drug resistance of the rectal bacteria is more serious. In order to improve the survival rate and fertility rate of piglets, it is suggested that the clinical medication in the pig farm be treated in time on the basis of the result of drug sensitivity test.