Abstract: 【Objective】 To develop an effective pregermination method of inducing ear bud in plastic bag culture of Auricularia auricular, and to provide theoretical basis and technical support for cultivation of Auricularia auricular.【Method】A comparative experiment was conducted on different covers and ventilation methods of mushroom bags in bacteria bed by using the method of perforated ear and concentrated germination.【Result】The germination rate of the active ventilation and moisturizing method was the highest, which was 93.28%. The germination rate was high in the case of natural ventilation and good humidity, which was over 80%. However, the sprouting rate was the worst in poor ventilation but good humidity condition, which was below 45%, and the pollution rate and the degree of burning bacteria were high.【Conclusion】Humidity, temperature and ventilation affect the pregermination effect of Auricularia auricula in plastic bags. The good ventilation could improve the germination rate and uniformity obviously and reduce the pollution rate and burning degree under the suitable temperature and humidity conditions.