Abstract: 【Objective】 To clarify the extraneous gene drift rule of BT Cry1Ac transgenic cotton and provide the basis for ecological safety evaluation of transgenic cotton, we carried out this study in Turpan and Korla for two years.【Method】Transgenic cotton was planted in the plot experiment, and the drift distance of exogenous gene was detected by using Kanamycin resistance detection combined with molecular detection the following year.【Result】These results showed that either the source of SGK321 or GK19, the exogenous gene Cry1Ac could be detected from 1-120 m. The maximum drift rate of SGK321 seed drift rate was 1 m from the edge of the transgenic cotton area in the south direction, and the drift rate was 5%. GK19 drift rate was biggest to the west up to 10.09%. So, based on direction and distance factors, Cry1Ac gene drift Logistic regression forecast model was built, and these results showed that the direction and distance of seed drift had a significant effect (P< 0.05), however, direction and distance could not ultimately determine the extraneous gene drift rate.【Conclusion】The extraneous gene drift rate of transgenic cotton was affected by direction and distance, but it was not the result of the combination of direction and distance that ultimately determined the extraneous gene drift rate.