Abstract: 【Objective】 To provide theoretical basis for the selection of pollinated varieties and genetic improvement of Korla pear by classifying and studying the S genotypes of 9 native pear varieties in Xinjiang.【Method】A specific primer was designed to amplify the genomic DNA of 9 native pear varieties in Xinjiang, including PCR amplification, fragment recovery, cloning, sequencing, and the analysis of each sequence by bioinformatics software.【Result】The S-genotypes of the seven cultivars were identified as follows: Hesejujuli S28S4, Luntaijujuli S28S4, Hesejujuli:S28S4,Luntaijujuli:S28S4,Huochengdonghuangli:S28S1,Bayueli:S28S4,Kuerlehuangsuanli:S22S35,Jujuli:S28S4,Kuikeamute1hao:S36S34S22.One gene was identified as S8Sx and another gene as S28Sx in Keteamute Pear and Qipanli Pear, respectively.【Conclusion】It has enriched the s-genotypes information of pear cultivars in China and provided a certain guidance gist for high yield cultivation and genetic improvement of Korla pear.