Abstract: 【Objective】 To verify the effectiveness of eIF4E1 gene vector constructed based on CRISPR/Cas9 system for targeted genome editing processing tomato (Solanum lycopersicum) and to provide technical support for the application of CRISPR/Cas9 system in the cultivation of PVY resistant plants.【Method】Constructing a CRISPR/Cas9 system expression vector targeting to the elF4E1 gene of tomato eukaryotic translation initiation factor, the tomato plants were transiently transformed by Agrobacterium tumefaciens infiltration method. The DNA sequences around the target sites of the transformed plants were amplified by PCR and digested with Hae III, the bands that had not been successfully digested were recovered and ligated with pGEM-T vector for monoclonal sequencing.【Result】An alignment analysis of the 9 cloned sequences revealed that mutations occurred at 6-8 bp upstream of the PAM (protospacer adjacent motifs), and they were all single-base substitutions, resulting in the replacement of a single amino acid in the polypeptide chain.【Conclusion】In this study, the vector constructed by CRISPR/Cas9 genome editing system can specifically target the tomato eIF4E1 gene, which has laid the foundation for the subsequent use of the CRISPR/Cas9 system to knock out the eIF4E1 gene and obtain the tomato breeding materials against PVY virus.