4种抗生素用于干木耳组织分离方法的评价

doi:10.6048/j.issn.1001-4330.2021.04.015

新疆农业科学 ›› 2021, Vol. 58 ›› Issue (4): 720-730.DOI: 10.6048/j.issn.1001-4330.2021.04.015

• 土壤肥料·植物保护·微生物 • 上一篇下一篇

4种抗生素用于干木耳组织分离方法的评价

陈艳琦¹, 吕艳聪¹, 贾培松², 张芳芳¹, 田龙¹, 宋冰¹, 李玉¹

1.吉林农业大学食药用菌教育部工程研究中心,长春 130118;
2.新疆农业科学院植物保护研究所/农业部西北荒漠绿洲作物有害生物综合治理重点实验室,乌鲁木齐 830091;
3.嘉兴市经济菌物新种质资源创制重点实验室,浙江嘉兴 314000

收稿日期:2020-12-04 出版日期:2021-04-20 发布日期:2021-04-25
通信作者: 宋冰(1980-),男,吉林人,讲师,博士,研究方向为食药用菌栽培及育种,(E-mail)song19800123@126.com
李玉(1944-)男,山东人,教授,博士,研究方向为菌物学,(E-mail)fungi966@126.com
作者简介:陈艳琦(1994-),女,吉林人,硕士研究生,研究方向为食药用菌栽培,(E-mail)cyqmogu@126.com
基金资助:
国家重点研发计划(2018YFD1001000;2017YFD0601002);公益性行业(农业)科研专项(201503137);“111”引智基地项目(D17014)

Evaluation of Four Antibiotics for Dry Auricularia Tissue Isolation

CHEN Yanqi¹, LÜ Yancong¹, JIA Peisong², ZHANG Fangfang¹, TIAN Long¹, SONG Bing¹, LI Yu¹

1. Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, Jilin Agricultural University, Changchun 130118, China;
2. Key Laboratory of Integrated Crop Pest Management in China North-western Desert Oasis,Ministry of Agriculture P. R. China, Urumqi, 830091, China

Received:2020-12-04 Online:2021-04-20 Published:2021-04-25
Correspondence author: SONG Bing(1980-), male, Doctor, native place: Jilin,Research field: Cultivation and breeding of edible and medicinal fungi, (E-mail)song19800123@126.com
LI Yu(1944-), male, native place: Shandong, Professor, Research field: Mycology, (E-mail)fungi966@126.com
Supported by:
National Key R & D Project of China (2018YFD1001000 and 2017YFD0601002 ); Special Fund for Public Welfare Industry (Agriculture) Scientific R &D Program of Ministry of Agriculture and Rural Affairs, P. R. China (201503137);“111”Talent Recruitment Base Project” (D17014)

摘要/Abstract

摘要： 【目的】 筛选出4种抗生素的使用方法及用于木耳的干制条件。【方法】 以野生及栽培木耳为供试材料,以干耳浸泡于抗生素75%酒精混合溶液消毒的方法进行组织分离。对比污染率、萌发率筛选4种抗生素的适宜使用方法和木耳干制方法,显微观察木耳不同部位的萌发能力。【结果】 4种抗生素的使用方法分别为:青霉素6 mg/mL消毒3~5 min;链霉素15 mg/mL消毒1~3 min;头孢曲松钠15 mg/mL浸泡3~5 min;庆大霉素1.5 mg/mL浸泡1~3 min,经65℃烘干8 h的处理后均可萌发,可育层、菌肉以及不育层均可观察到菌丝萌发。【结论】 4种抗生素均可用于干木耳菌种获得,65℃烘干木耳可用于组织分离,未经长时间高温处理的新鲜子实体干燥后,于室温下保存1年仍可分离成功,木耳具有周身萌发的能力。

关键词: 黑木耳; 毛木耳; 抗生素; 组织分离; 烘干温度; 萌发面

Abstract: 【Objective】 To screen out suitable working concentration of 4 antibiotics and drying condition for dry Auricularia fruiting bodies. 【Methods】 In this study, fresh and dry fruiting bodies of wild and cultivated were used as materials, the dry ear was soaked in 75% alcohol mixture solution of antibiotics for tissue separation. The germination ability of different parts of Auricularia auricula was studied by microscopic observation through comparing the contamination rate and germination rate of 4 antibiotics and the dry method of Auricularia auricular. 【Results】 The results showed that the suitable concentration and disinfection time of the four antibiotics were: 6 mg/mL and 3-5 min for penicillin; 15 mg/mL and 1-3 min for streptomycin; 15 mg/mL and 3-5 min for ceftriaxone sodium; 1.5 mg/mL and 1-3 min for gentamicin. The fruiting bodies after drying at 65℃ for 8 hours could be isolated successfully. Mycelium germination was observed in the fertile layer, hypophyll and sterile layer after drying for 8 h at 65℃. 【Conclusion】 All of the four antibiotics can be used to obtain dried Auricularia fruiting and temperature 65℃ can be used to isolate its tissue. After drying the fresh fruiting body without long time high temperature treatment, it can be separated successfully at room temperature for one year. Auricularia heimuer has the ability to germinate around the body.

Key words: Auricularia heimuer; Auricularia cornea; antibiotic; tissue isolation; drying temperature; germination surface

中图分类号:

S646
S188

陈艳琦, 吕艳聪, 贾培松, 张芳芳, 田龙, 宋冰, 李玉. 4种抗生素用于干木耳组织分离方法的评价[J]. 新疆农业科学, 2021, 58(4): 720-730.

CHEN Yanqi, LÜ Yancong, JIA Peisong, ZHANG Fangfang, TIAN Long, SONG Bing, LI Yu. Evaluation of Four Antibiotics for Dry Auricularia Tissue Isolation[J]. Xinjiang Agricultural Sciences, 2021, 58(4): 720-730.

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4种抗生素用于干木耳组织分离方法的评价

Evaluation of Four Antibiotics for Dry Auricularia Tissue Isolation

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