几种不同巴旦木花粉生活力及柱头可授性比较分析

doi:10.6048/j.issn.1001-4330.2020.08.009

新疆农业科学 ›› 2020, Vol. 57 ›› Issue (8): 1450-1456.DOI: 10.6048/j.issn.1001-4330.2020.08.009

几种不同巴旦木花粉生活力及柱头可授性比较分析

张翔飞¹, 岳婉婉¹, 刘泉鑫¹, 曾斌¹, 郝庆², 阿布都卡尤木·阿依麦提¹

1.新疆农业大学林学与园艺学院, 乌鲁木齐 830052;
2.新疆农业科学院园艺作物研究所,乌鲁木齐 830091

收稿日期:2019-12-02 发布日期:2020-09-01
通信作者: 曾斌(1970-),男,湖北人,副教授,博士,硕士生导师,研究方向为果树种质资源及果树栽培生理,(E-mail)zbxnd@163.com 郝庆(1969-),男,新疆阿克苏人,研究员,硕士生导师,研究方向为新疆特色果树栽培,(E-mail)haoqingxj@sohu.com
作者简介:张翔飞(1994-),男,河南禹州人,硕士研究生,研究方向为园艺作物栽培,(E-mail)1185251971@qq.com
基金资助:
新疆维吾尔自治区自然科学基金“新疆巴旦木自交不亲和非S基因关键因子SSK1的克隆及其功能分析研究”(2019D01A56);国家自然科学基金“基于S-核酸酶的新疆野生扁桃自交不亲和性泛素化特性的分子机制研究”(31660557);新疆维吾尔自治区园艺学重点学科“新疆特色果树种质资源开发利用与优良品种选育推广”(2016-10758-3)

Comparative Study on Pollen Viability and Stigma Availability (Prunus dulcis)

ZHANG Xiangfei¹, YUE Wanwan¹, LIU Quanxin¹, ZENG Bin¹, HAO Qing², Abudoukayoumu Ayimaiti¹

1. College of Forestry and Horticulture, Xinjiang Agricultural University, Urumqi 830052, China;
2. Institute of Horticulture Crops,Xinjiang Academy of Agricultural Sciences,Urumqi 830091, China

Received:2019-12-02 Published:2020-09-01
Correspondence author: ZENG Bin (1970-), male, Hubei native, associate professor, doctor, master's supervisor, research direction is fruit tree germplasm resources and fruit tree cultivation physiology, (E-mail) zbxnd@163.com HAO Qing (1969-), male, Aksu, Xinjiang, researcher, master's supervisor, research direction is Xinjiang characteristic fruit tree cultivation, (E-mail) haoqingxj@sohu.com
Supported by:
Supported by the Natural Science Foundation Project of Xinjiang Uygur Autonomous Region “Cloning and Functional Analysis of SSK1, a Key Factor of Self-incompatibility and Non-S Gene in Almonds (Prunus dulcis) in Xinjiang” (2019D01A56); the National Natural Science Foundation of China “Study on Molecular Mechanism of Self-incompatibility Ubiquitin Characteristics of Xinjiang Wild Almonds (Prunus dulcis) Based on S-nuclease” (31660557); Key Discipline Project of Horticulture in Xinjiang Uygur Autonomous Region “Development and Utilization of Characteristic Fruit Tree Germ Resources and Breeding and Popularization of Excellent Varieties in Xinjiang” (2016-10758-3)

摘要/Abstract

摘要： 【目的】 研究巴旦木花粉生活力,比较分析柱头可授性,为巴旦木人工授粉及相关研究和巴旦木良种选育提供理论依据。【方法】 以6个国内外巴旦木品种为材料,采用I₂-KI染色法、离体萌发法法测定不同开花天数的巴旦木花粉活力的变化;用联苯胺-过氧化氢法测定巴旦木各品种的柱头可授性。【结果】 2种方法测定的花粉活力最大的为米星,最小的为麻壳;石头、尖嘴黄、鹰嘴的雌蕊在花开放后第2 d花柱长度达到最大且具有较强的可授性,米星、布特、麻壳的雌蕊在花开放第3 d花柱长度达到最大且具有较强的可授性。【结论】 采集花后第2 d的花粉对花后第2 d的石头、尖嘴黄、鹰嘴和第3 d的米星、布特、麻壳柱头进行人工授粉。

关键词: 巴旦木; 花粉活力; 柱头可授性

Abstract: 【Objective】 To provide a reference and theoretical support for the practice of artificial pollination and related research of almonds in the hope of providing a preliminary theoretical basis for the further smooth study of affinity pollination.【Method】 Six almonds varieties at home and abroad were used as experimental materials, I₂-KI staining method was used to determine the changes of pollen activity of almonds varieties on different anthesis days, and benzidine hydrogen peroxide method was used to determine the stigmatization of almonds varieties.【Result】 The pollen vigor determined by the two methods was the largest and the smallest was Make; the pistil length of Shitou, Jianzuihuang and Yingzui reached the maximum and strong acceptability on the second day after anthesis, and the pistil length of Mission, Butter and Make reached the maximum and had strong acceptability on the 3rd day after anthesis , and the pistil length of Shitou, Jianzuihuang and Yingzui reached the maximum and strong acceptability on the 3rd day after anthesis, and the pistil length of Mission, Butter and Make reached the maximum and strong acceptability on the 3rd day after anthesis.【Conclusion】 The pollen collected on the second day after anthesis and the Shitou on the 2nd day after anthesis, Jianzuihuan, Yingzui and the Mission, Butter and Make stigmas on the 3rd day were artificially powdered, which provided the theoretical basis for the breeding of almond varieties.

Key words: almond; pollen vitality; stigma receptivity

中图分类号:

S644.9

张翔飞, 岳婉婉, 刘泉鑫, 曾斌, 郝庆, 阿布都卡尤木·阿依麦提. 几种不同巴旦木花粉生活力及柱头可授性比较分析[J]. 新疆农业科学, 2020, 57(8): 1450-1456.

ZHANG Xiangfei, YUE Wanwan, LIU Quanxin, ZENG Bin, HAO Qing, Abudoukayoumu Ayimaiti. Comparative Study on Pollen Viability and Stigma Availability (Prunus dulcis)[J]. Xinjiang Agricultural Sciences, 2020, 57(8): 1450-1456.

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几种不同巴旦木花粉生活力及柱头可授性比较分析

Comparative Study on Pollen Viability and Stigma Availability (Prunus dulcis)

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