野山杏总黄酮对LPS诱导鸡肝炎的影响

doi:10.6048/j.issn.1001-4330.2023.08.029

摘要/Abstract

摘要：

【目的】研究野山杏总黄酮对LPS诱导鸡肝炎的影响。【方法】给予鸡腹腔注射5 mg/kg LPS制备急性肝损伤(ALI)模型,以生理盐水为空白对照组,吲哚美辛为阳性对照组和野山杏总黄酮低(50 mg/kg)、中(100 mg/kg)、高(200 mg/kg)剂量组为试验组,测定鸡体温、体重的变化和血液学参数及血液生化指标,观察鸡肝脏病理,分析野山杏总黄酮对鸡肝炎的影响。【结果】野山杏总黄酮各剂量组可不同程度降低LPS导致的体温升高;缓解了炎症造成的体重减轻;显著或极显著(P<0.05,P<0.01)逆转LPS导致的炎性细胞增加,其中野山杏总黄酮各剂量组对白细胞数的减少具有剂量依赖性,对淋巴细胞、粒细胞及中间细胞不呈现剂量依赖性;野山杏总黄酮各剂量组均可抑制炎症条件下ALT、AST的水平;中高剂量组能明显改善LPS引起的鸡肝组织病理损伤。【结论】野山杏总黄酮各剂量组均能恢复炎症导致的体温升高,对鸡体重有促进作用,能减少炎性细胞的数量,抑制ALT、AST含量升高,改善LPS致鸡肝炎的病理损伤,具有一定的抗肝炎作用。

关键词: 野山杏; 黄酮类化合物; LPS; 肝炎; 黄羽肉鸡

Abstract:

【Objective】 To study the effect of total flavone of wild apricot on lPS-induced chicken hepatitis.【Methods】 Acute liver injury (ALI) model was established by intraperitoneal injection of LPS at 5 mg/kg in chickens and normal saline was used as blank control group, indomethacin as positive control group, and low (50 mg/kg), medium (100 mg/kg) and high (200 mg/kg) dose groups of total flavonoids of wild apricot were taken orally as experimental groups to analyze the effect of total flavonoids of wild apricot on chicken hepatitis.【Results】 Total flavonoids of apricot in each dose group could reduce the temperature rise caused by LPS to different degrees and relieve weight loss caused by inflammation; The increase of inflammatory cells induced by LPS was significantly or extremely significantly reversed (P<0.05, P<0.01).The decrease of WBC was dose-dependent in each dose group, but not in lymphocyte, granulocyte and intermediate cells.The levels of ALT and AST in all dosage groups were inhibited.At the same time, the liver histopathological injury induced by LPS could be significantly improved in the medium and high dose group.【Conclusion】 Total flavonoids of wild apricot in all dose groups can restore the increase of body temperature caused by inflammation and promote the weight of chickens, thus reducing the number of inflammatory cells, inhibiting the increase of ALT and AST and improving the pathological damage of chicken hepatitis caused by LPS, so conclusion can be reached that the total flavonoids of wild apricot can have a certain anti hepatitis effect, and be of a certain practical significance for the actual health of domestic poultry breeding.

Key words: wild apricot; flavonoids; LPS; hepatitis; yellow broiler chicks

中图分类号:

S662.2

束佳敏, 郭元印, 卫丁一, 刘浩然, 依斯马依力, 黄磊, 何子涵, 姚刚, 戴小华. 野山杏总黄酮对LPS诱导鸡肝炎的影响[J]. 新疆农业科学, 2023, 60(8): 2066-2073.

SHU Jiamin, GUO Yuanyin, WEI Dingyi, LIU Haoran, Yisimayili , HUANG Lei, HE Zihan, YAO Gang, DAI Xiaohua. Effects of Total Flavonoids of Wild Apricot on LPS-induced Chicken Hepatitis[J]. Xinjiang Agricultural Sciences, 2023, 60(8): 2066-2073.

图/表 6

参考文献 27

[1]	张加延, 何跃. 我国“三北”杏树产业带的发展现状[J] 北方果树, 2007,(1):33-35.
	ZHANG Jiayan, HE Yue. Development status of our country "three North" apricot tree industrial belt[J]. Northern Fruits, 2007,(1): 33-35.
[2]	田丝雨, 杨晓君, 何笑娜, 等. 伊吾县野山杏仁多肽的提取及其抗氧化活性的研究[J] 中国食品添加剂, 2018,(4):74-79.
	TIAN Siyu, YANG Xiaojun, HE Xiaona, et al. Study on wild mountain almond polypeptide extraction and its antioxidant activity in Yiwu County[J]. China Food Additives, 2018,(4): 74-79.
[3]	郭永霞, 王丽艳, 殷奎德. 山杏果肉水溶性多糖提取工艺参数优化[J]. 食品科学, 2010, 31(14):116- 119. DOI
	GUO Yongxia, WANG Liyan, YIN Kuide. Optimization of mountain apricot pulp water-soluble polysaccharide extraction process parameters[J]. Food Science, 2010, 31(14): 116-119. DOI
[4]	张杰, 张海珊, 田丝雨, 等. 新疆伊吾县野山杏叶的品质检测与分析[J] 华西药学杂志, 2018, 33 (4): 413-416.
	ZHANG Jie, ZHANG Haishan, TIAN Siyu, et al. Quality Detection and Analysis of Yishan Apricot Leaves in Yiwu County, Xinjiang[J]. West China Pharmaceutical Sciences, 2018, 33(4): 413-416.
[5]	Fangling Qin, et al. Phenolic composition, antioxidant and antibacterial properties, and in vitro anti-HepG2 cell activities of wild apricot (Armeniaca Sibirica L. Lam) kernel skins[J]. Food and Chemical Toxicology, 2019, 129: 354-364. DOI PMID
[6]	萨茹丽, 杨斌, 玉梅, 等. 沙葱总黄酮体外抗菌、抗病毒、抗肿瘤活性的研究[J] 饲料工业, 2018, 39(8):54-58.
	SA Ruli, YANG Bin, YU Mei, et al. Study on in vitro antibacterial, antiviral and anti-tumumor activity of total flavonoids[J]. Feed Industry, 2018, 39 (8): 54-58.
[7]	Wang T, Zhang P, Lv H, et al. A Natural Dietary Flavone Myricetin as an α-Hemolysin Inhibitor for Controlling Staphylococcus aureus Infection[J]. Frontiers in Cellular and Infection Microbiology, 2020, 10: 330. DOI URL
[8]	阿依姑丽·艾合麦提, 张华, 等. 野山杏水不溶性膳食纤维的降血脂和保肝作用的研究[J] 时珍国医国药, 2012, 23(4):928-930.
	Aiyiguli Aihemaiti, ZHANG Hua, et al. Study on the blood lipid lowering and liver preservation effect of wild apricot water insoluble dietary fiber[J]. Lishizhen Medicine and Materia Medica Research, 2012, 23(4): 928-930.
[9]	阿依姑丽·艾合麦提, 王妙颖, 彭禛菲, 等. 野山杏果肉总有机酸对高脂血症大鼠血脂及相关基因表达的影响[J] 食品科学, 2018, 39(21):171-176. DOI
	Aiyiguli Aihemaiti, WANG Miaoying, PENG Zhen fei, et al. Effect of total organic acid on blood lipid and related gene expression in hyperlipidemia rats[J]. Food Science, 2018, 39(21): 171-176. DOI URL
[10]	付婷婷, 李慧, 吴洪军, 等. 山杏和山丁子多酚的抗氧化活性研究[J]. 林业科技, 2016, 41(4):60-63.
	FU Tingting, LI Hui, WU Hongjun, et al. Study on antioxidant activity of apricot and seed polyphenols[J]. Forestry Science and Technology, 2016, 41 (4): 60-63.
[11]	戴小华, 谷虹霏, 金科旭, 等. 应用RP-HPLC法测定野山杏果肉黄酮类化合物[J] 新疆农业科学, 2021, 58(8):1476-1485. DOI
	DAI Xiaohua, GU Hongfei, JIN Kexu, et al. Application of RP-HPLC method[J]. Xinjiang Agricultural Sciences, 2021, 58(8): 1476-1485. DOI
[12]	谷虹霏, 戴小华, 阿依姑丽·艾合麦提, 等. 新疆野山杏总黄酮对小鼠镇痛作用考察[J]. 中医药信息, 2018, 35(4):30-33.
	GU Hongfei, DAI Xiaohua, Aiyiguli Aihemaiti, et al. Study on the analgesic effect of total flavonoids from Xinjiang wild apricot on mice[J]. Chinese Journal of Information on Traditional Chinese Medicine, 2018, 35 (4): 30-33.
[13]	戴小华, 阿依姑丽·艾合麦提, 谷虹霏, 等. 野山杏总黄酮抗急性炎症作用研究[J] 新疆农业大学学报, 2017, 40(2):106-110.
	DAI Xiaohua, Aiyiguli Aihemaiti, GU Hongfei, et al. Study on the anti-acute inflammatory effect of total flavonoids[J]. Journal of Xinjiang Agricultural University, 2017, 40(2): 106-110.
[14]	阿依姑丽·艾合麦提, 戴小华, 张华, 等. 野山杏总黄酮对高血脂大鼠降血脂及抗氧化作用的研究[J] 西北药学杂志, 2014, 29(3):258-261.
	Aiyiguli Aihemaiti, DAI Xiaohua, ZHANG Hua, et al. Study on the lipid-lowering and antioxidant effects of total flavonoids on high blood lipids in rats[J]. Northwest Pharmaceutical Journal, 2014, 29(3): 258-261.
[15]	管晓辉, 夏炎, 崔艳艳, 等. 多毛橐吾总黄酮的提取及其抗炎机制研究[J] 西北农林科技大学学报(自然科学版), 2017, 45(11):9-17.
	GUAN Xiaohui, XIA Yan, CUI Yanyan, et al. Study on the extraction of ligularia total flavonoids and its anti-inflammatory mechanism[J] Journal of Northwest A & F University (Natural Science Ed.), 2017, 45(11):9-17.
[16]	周康盛, 田雯, 张振, 等. 黄花棘豆化学成分及生物活性研究进展[J] 天然产物研究与开发, 2020, 32(1):161-171.
	ZHOU Kangsheng, TIAN Wen, ZHANG Zhen, et al. Progress in the chemical composition and biological activities of yellow soybean[J]. Natural Product Research and Development, 2020, 32(1): 161-171.
[17]	赵兴辉, 叶柳凤, 林小田. 镰形棘豆总黄酮灌胃对乙型肝炎大鼠肝损伤的改善作用及其机制[J] 山东医药, 2020, 60(21):44-47.
	ZHAO Xinghui, YE Yufeng, LIN Xiaotian. Effect and mechanism of gastric improvement on liver injury in hepatitis B rats[J]. Shandong Medical Journal, 2020, 60(21): 44-47.
[18]	雷斯瑀, 肖霜艳, 叶芮伶, 等. 赶黄草总黄酮的提取及其对小鼠酒精性肝损伤的治疗作用[J] 吉林畜牧兽医, 2018, 39(6):5-8,11.
	LEI Siyu, XIAO Shuangyan, YE Ruiling, et al. Extraction of total flavonoids and their therapeutic effects on alcoholic liver injury in mice[J]. Jilin Animal Husbandry and Veterinary Medicine, 2018, 39(6): 5-8,11.
[19]	刘舒凌, 吴燕春, 钟振国, 等, 饿蚂蝗总黄酮对鸭乙型肝炎的保护作用研究[J]. 中药药理与临床, 2017, 33(6):76-79.
	LIU Shuling, WU Yanchun, ZHONG Zhenguo, et al. Research on the protective effect of total flavonoids on duck hepatitis B[J]. Pharmacology and Clinical Practice of Traditional Chinese Medicine, 2017, 33 (6): 76-79.
[20]	李晓东, 陈芝芸, 俞建顺, 等. 山楂叶总黄酮对非酒精性脂肪性肝炎大鼠组织COX-2/Nrf2表达的影响[J] 中国中药杂志, 2016, 41(4):711-715.
	LI Xiaodong, CHEN Zhiyun, YU Jianshun, et al. Effect of total flavonoids in hawthorn leaves on COX-2 / Nrf2 expression in rats with nonalcoholic steatohepatitis[J]. Chinese Journal of Traditional Chinese Medicine, 2016, 41(4): 711-715.
[21]	郭雅迪, 李洋洋, 刘芳芳, 等. 减轻脂多糖诱导炎症的中药有效成分的研究进展[J] 华西药学杂志, 2021, 36(3):331-335.
	GUO Yadi, LI Yangyang, LIU Fangfang, et al. Progress in the active constituents of traditional Chinese medicine for alleviating LPS-induced inflammation[J]. Huaxi Pharmaceutical Journal, 2021, 36(3): 331-335.
[22]	Zhong M, Zhang X, Shi X, et al. Halofuginone inhibits LPS-induced attachment of monocytes to HUVECs[J]. International Immunopharmacology, 2020, 87:106753. DOI URL
[23]	马冉, 姜世俞. 家畜肝脏及腹膜疾病的症状及治疗[J] 湖北畜牧兽医, 2015, 36(6):28-29.
	MA Ran, JIANG Shiyu. Symptoms and treatment of liver and peritoneal diseases in livestock[J]. Hubei Animal Husbandry and Veterinary Medicine, 2015, 36(6): 28-29.
[24]	金虹新. 血常规检验中不同采血方法的应用效果分析[J] 中国实用医药, 2021, 16(5):208-210.
	JIN Hongxin. Analysis of the application effect of different blood collection methods in the routine blood test[J]. Chinese Practical Medicine, 2021, 16(5): 208-210.
[25]	陈晓兵. 肝硬化患者血细胞参数变化特点研究[J] 现代诊断与治疗, 2014, 25(5):985-987.
	CHEN Xiaobing. Study on blood cell parameters changes in liver cirrhosis patients[J]. Modern Diagnosis and Treatment, 2014, 25(5): 985-987.
[26]	梁萍, 于杰. 肝病超声诊断指南[J] 临床肝胆病杂志, 2021, 37(8):1770-1785,1993-1994.
	LIANG Ping, YU Jie. Guidelines for Ultrasound Diagnosis of Liver Disease[J]. Journal of Clinical Hepatobiliary Diseases, 2021, 37(8): 1770-1785,1993-1994.
[27]	Roushan M R H, Hajiahmadi M, Shafaie S. Histopathological features of liver and its relation to serum transaminase levels in 91 cases of anti-HBe-positive chronic hepatitis B[J]. International Journal of Clinical Practice, 2010, 59(7): 791-794. DOI URL

指标 Indicators	空白对照组 Blank control group	模型组 Model group	低剂量组 Low dose group	中剂量组 Dose group	高剂量组 High dose group	阳性对照组 Positive group
白细胞总数 Total leucocyte count (10⁹/L)	89.81±3.05	114.94±1.27^##	86.54±1.40^**	83.38±2.88^**	81.57±1.70^**	83.37±2.83^△△
淋巴细胞数 Lymphocyte count (10⁹/L)	73.08±2.63	82.73±3.10^##	68.93±1.23^**	69.73±3.26^**	68.57±1.34^**	68.42±2.37^△△
中间细胞数 Intermediate cell number (10⁹/L)	7.13±0.28	8.41±0.25^##	7.71±0.20^*	6.99±0.23^**	6.60±0.24^**	6.61±0.28^△△
粒细胞数 Granulocyte count (10⁹/L)	7.50±0.30	10.59±0.79^##	8.88±0.50^*	6.69±0.45^**	7.38±0.46^**	7.61±0.39^△△
红细胞数 Red blood cell count (10¹²/L)	2.03±0.10	2.42±0.10^##	2.56±0.04	2.41±0.08	1.82±0.11^**	2.34±0.08
红细胞压积 Hematocrit (%)	26.93±1.24	31.53±1.73^##	32.08±0.43	30.14±0.84	23.15±1.27^**	29.55±1.16
红细胞平均体积 Mean erythrocyte volume (fL)	132.53±1.33	124.79±0.84^##	126.18±0.77	127.46±1.31	130.78±1.07^**	126.46±1.16
红细胞分布宽度SD Red cell distribution width SD (%)	41.02±0.71	36.43±0.31^##	36.63±0.69	36.54±0.65	38.29±0.74^*	36.64±0.50
红细胞分布宽度CV Red cell distribution width CV (%)	10.89±0.21	10.15±0.07^##	10.09±0.15	9.97±0.15	10.22±0.25	9.91±0.22
血红蛋白含量 Hemoglobin content (pg)	68.63±1.71	56.73±0.50^##	60.56±1.08	64.73±2.54^**	78.25±4.17^**	60.16±1.09
血红蛋白 Hemoglobin(g/L)	151.15±4.54	139.64±3.33^#	155.14±2.52^**	152.86±3.20^**	150.79±3.19^*	144.45±5.85
血红蛋白浓度 Hemoglobin concentration (g/L)	518.31±11.00	455.60±4.85^##	480.57±8.19	504.15±9.62^**	604.18±29.24^**	474.15±7.50

指标 Indicators	空白对照组 Blank control group	模型组 Model group	低剂量组 Low dose group	中剂量组 Dose group	高剂量组 High dose group	阳性对照组 Positive group
白细胞总数 Total leucocyte count (10⁹/L)	89.81±3.05	114.94±1.27^##	86.54±1.40^**	83.38±2.88^**	81.57±1.70^**	83.37±2.83^△△
淋巴细胞数 Lymphocyte count (10⁹/L)	73.08±2.63	82.73±3.10^##	68.93±1.23^**	69.73±3.26^**	68.57±1.34^**	68.42±2.37^△△
中间细胞数 Intermediate cell number (10⁹/L)	7.13±0.28	8.41±0.25^##	7.71±0.20^*	6.99±0.23^**	6.60±0.24^**	6.61±0.28^△△
粒细胞数 Granulocyte count (10⁹/L)	7.50±0.30	10.59±0.79^##	8.88±0.50^*	6.69±0.45^**	7.38±0.46^**	7.61±0.39^△△
红细胞数 Red blood cell count (10¹²/L)	2.03±0.10	2.42±0.10^##	2.56±0.04	2.41±0.08	1.82±0.11^**	2.34±0.08
红细胞压积 Hematocrit (%)	26.93±1.24	31.53±1.73^##	32.08±0.43	30.14±0.84	23.15±1.27^**	29.55±1.16
红细胞平均体积 Mean erythrocyte volume (fL)	132.53±1.33	124.79±0.84^##	126.18±0.77	127.46±1.31	130.78±1.07^**	126.46±1.16
红细胞分布宽度SD Red cell distribution width SD (%)	41.02±0.71	36.43±0.31^##	36.63±0.69	36.54±0.65	38.29±0.74^*	36.64±0.50
红细胞分布宽度CV Red cell distribution width CV (%)	10.89±0.21	10.15±0.07^##	10.09±0.15	9.97±0.15	10.22±0.25	9.91±0.22
血红蛋白含量 Hemoglobin content (pg)	68.63±1.71	56.73±0.50^##	60.56±1.08	64.73±2.54^**	78.25±4.17^**	60.16±1.09
血红蛋白 Hemoglobin(g/L)	151.15±4.54	139.64±3.33^#	155.14±2.52^**	152.86±3.20^**	150.79±3.19^*	144.45±5.85
血红蛋白浓度 Hemoglobin concentration (g/L)	518.31±11.00	455.60±4.85^##	480.57±8.19	504.15±9.62^**	604.18±29.24^**	474.15±7.50