泰山磐石散加减方对妊娠不同阶段英纯血马血液生理生化指标的影响

doi:10.6048/j.issn.1001-4330.2022.06.028

摘要/Abstract

摘要：

【目的】 研究复方中草药泰山磐石散加减方(TSPSS)对妊娠不同阶段英纯血母马血液指标的影响。【方法】 选取妊娠7个月的英纯血马19匹,体重480~550 kg,以妊娠阶段分组,分别于妊娠的第210、240、270和300 d 4个阶段连续5 d补喂TSPSS,在喂药前、喂药结束后12 h采集血液,检测血液常规、血液生化及孕酮和雌激素等指标进行检测,对比5个阶段纯血马血液指标差异性。【结果】 WBC在第三阶段显著高于用药前(P<0.05),但在正常范围内;MID和LYMPH在第四阶段显著低于用药前和其他各阶段(P<0.05);LYM比例在第四阶段极显著低于用药前和其他各阶段(P<0.01);GRAN及GRAN比例在4个阶段中呈显著升高的趋势(P<0.05),第四阶段极显著高于第一阶段(P<0.01)。ALB含量在第三阶段、第四阶段显著低于用药前(P<0.05);TBIL含量第二阶段显著高于用药前和其他各阶段(P<0.05);Ca²⁺第四阶段显著高于第二阶段(P<0.05)。E₂在第四阶段显著低于用药前(P<0.05),4个阶段与用药前相比呈降低趋势。【结论】 妊娠7~10月龄英纯血马补饲中草药TSPSS散加减方对肝肾功能无影响,能够有效增强孕马后期机体的免疫力及代谢水平,促进营养物质的吸收,具有保胎效果。

关键词: 泰山磐石散; 妊娠; 英纯血马; 血液指标

Abstract:

【Objective】 The study is aimed to investigate the effect of TSPSS on blood indexes of British thoroughbred horses in different pregnant stages. 【Method】 The number of 19 pregnant 7 month British thoroughbred horses of 480-550 kg heavy were selected in four groups according pregnant stage. TSPSS were supplemented for 5 consecutive days at 210, 240, 270 and 300 th pregnant day. Before administration and after being administrated 12 hours, the blood samples were collected and then blood routine, blood biochemistry, progesterone, estrogen and other indicators were tested to analyze the difference of blood indicators on five stages horses. 【Result】 The results showed that WBC was significantly higher than before (P< 0.05) in the third stage, but within the normal range. MID and LYMPH phases in the fourth phase were significantly lower than those before medication and the other phases (P < 0.05); The proportion of LYM in the fourth stage was significantly lower than that before and in other stages (P< 0.01); GRAN and GRAN ratio showed a significant increase in the four stages (P< 0.05), compared to that before, especially, the fourth stage was significantly higher than the first stage (P< 0.01). ALB in the third and fourth stages were significantly lower than that before treatment (P< 0.05); TBIL in the second stage was significantly higher than that before treatment and other stages (P< 0.05). The fourth stage of Ca²⁺ was significantly higher than that of the second stage (P< 0.05). E₂ in the fourth stage was significantly lower than that before treatment (P<0.05). 【Conclusion】 The results showed that the supplement of Chinese herbal medicine TSPSS had no effect on liver and kidney function of British thoroughbred horses aged from 7 to 10 months of gestation, which could effectively enhance the immunity and metabolism level of the body in the later period of pregnancy, promote the absorption of nutrients and have an effect on protecting fetus.

Key words: Taishan Panshi San; pregnancy; thoroughbred; blood index

中图分类号:

S821.2

买占海, 卢亚宾, 李建龙, 李晓斌, 徐文慧, 李海, 况玲. 泰山磐石散加减方对妊娠不同阶段英纯血马血液生理生化指标的影响[J]. 新疆农业科学, 2022, 59(6): 1553-1560.

MAI Zhanhai, LU Yabin, LI Jianlong, LI Xiaobin, XU Wenhui, LI Hai, KUANG Ling. Effects of Taishan Panshi San on Blood Physiological and Biochemical Indexes in Different Pregnant Stages of Thoroughbred Horses[J]. Xinjiang Agricultural Sciences, 2022, 59(6): 1553-1560.

图/表 5

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时间Time	饲喂草料Feeding forage
07:00	牧草干草
08:50~09:00	精饲料
11:30	苜蓿&干草(1∶1)
14:30	苜蓿干草
18:00	苜蓿干草
20:00	牧草干草
22:00	精饲料
00:00	牧草干草

时间Time	饲喂草料Feeding forage
07:00	牧草干草
08:50~09:00	精饲料
11:30	苜蓿&干草(1∶1)
14:30	苜蓿干草
18:00	苜蓿干草
20:00	牧草干草
22:00	精饲料
00:00	牧草干草

日粮组成 Ingredients	比例 Ratio	营养水平 Nutrient level	含量 Content
苜蓿干草 Alfalfa	40.00	有机物 OM	90.14
牧草干草 Hay	40.00	粗蛋白质 CP	19.59
玉米 Corn	10.00	中性洗剂纤维 NDF	44.23
麸皮 Wheat bran	2.60	酸性洗剂纤维 ADF	19.85
次粉 Wheat midding	1.40	钙 Ca	1.01
大豆粉 Soybean meal	5.20	磷 P	0.43
磷酸氢钙 CaHPO₄	0.40
食盐 NaCl	0.20
石粉 Limestone	0.20
合计 Total	100.00

日粮组成 Ingredients	比例 Ratio	营养水平 Nutrient level	含量 Content
苜蓿干草 Alfalfa	40.00	有机物 OM	90.14
牧草干草 Hay	40.00	粗蛋白质 CP	19.59
玉米 Corn	10.00	中性洗剂纤维 NDF	44.23
麸皮 Wheat bran	2.60	酸性洗剂纤维 ADF	19.85
次粉 Wheat midding	1.40	钙 Ca	1.01
大豆粉 Soybean meal	5.20	磷 P	0.43
磷酸氢钙 CaHPO₄	0.40
食盐 NaCl	0.20
石粉 Limestone	0.20
合计 Total	100.00

检测指标 Items	用药前 Before treatment	第1阶段 First stage	第2阶段 Second stage	第3阶段 Third stage	第4阶段 Fourth stage	参考范围^[7] Reference range
白细胞总数 WBC(10⁹/L)	4.10±0.44^Ba	4.91±0.50^ABa	4.33±0.30^Ba	6.53±0.9^Aa	5.12±0.50^ABa	6.00~12.00
淋巴细胞 LYMPH(10⁹/L)	2.12±0.33^ab	2.80±0.40^Aab	1.75±0.20^Aab	2.99±0.7^Aa	1.76±0.18^Bab	1.50~5.00
中间细胞 MID(10⁹/L)	0.48±0.06^Aa	0.52±0.06^Aa	0.40±0.04^Aab	0.62±0.04^Aa	0.27±0.07^Bb	0.00~0.60
粒细胞 GRAN(10⁹/L)	1.49±0.16^Aa	1.59±0.20^Aa	2.10±0.20^BCbc	2.91±0.30^Bb	4.10±0.40^Cc	3.00~6.00
淋巴细胞比例 LYM(%)	48.20±5.10^Aa	54.71±4.90^Aa	40.70±3.20^Ba	42.51±3.10^ABa	24.96±3.22^Cb	25.00~40.00
中间细胞比例 MID(%)	11.50±0.74^Aa	10.41±0.60^Aa	10.7±0.51^Aa	10.83±0.97^Aa	5.26±1.47^Bb	1.00~8.00
粒细胞比例 GRAN(%)	40.20±5.05^A	34.80±4.96^Aa	48.50±3.47^Bb	46.70±2.85^BCb	79.78±4.57^Cb	30.00~75.00
红细胞数 RBC(10¹²/L)	10.10±0.20^Aa	11.00±0.24^Aa	11.60±0.19^Aa	11.18±0.13^Aa	11.15±.15^Aa	6.00~12.00
血红蛋白 HGB(g/L)	160.00±5.30^Aa	166.00±5.12^Aa	170.00±4.48^Aa	167.00±5.60^Aa	171.00±12.69^Aa	100.00~180.00