关键词: COI基因序列; 雄性外生殖器; 物种鉴定; 整合分类学

Abstract: 【Objective】 Kirinia eversmanni, a newly recorded species in China, is identified by DNA bar-coding combined with morphology, which is likely to provide a research basis for its accurate identification and a reference for the study of butterfly’s biology and ecology.【Methods】 The Kirinia eversmanni adult abdomen was used to extract genomic DNA, mitochondrial Cytochrome Oxidase I (COI) gene was applied as a general purpose primer for PCR amplification, the sequence as its DNA barcode to anatomize male genitalia and describe the characteristics of male genitalia morphological structure and the surface of the wing.【Result】 The COI gene sequence length of Kirinia eversmanni was 696 bp, and the similarity of sequence between Kirinia eversmanni and the known sequences in NCBI was up to 99.70%, which could prove that the species studied was the same one as the species recorded in NCBI, Kirinia eversmanni, and also reflected the authenticity and reliability of the experimental sequence.At the same time, the morphological characteristics of aedeagus, tegumen and valvae structures of male genitalia were described and combined with the obtained sequences, which could be used for accurate identification evidence of Kirinia eversmanni.【Conclusion】 DNA bar-coding sequence combined with morphological description makes the identification result of Kirinia eversmanni more comprehensive and accurate.

Key words: COI gene sequence; male genitalia; species identification; integrated taxonomy