新疆2种苜蓿叶斑病病原菌主要生物学特性

doi:10.6048/j.issn.1001-4330.2020.10.011

新疆农业科学 ›› 0, Vol. ›› Issue (): 1854-1861.DOI: 10.6048/j.issn.1001-4330.2020.10.011

新疆2种苜蓿叶斑病病原菌主要生物学特性

窦晓丽¹, 胡文静¹, 刘凡², 林彩英¹, 李克梅¹

1.新疆农业大学农学院,乌鲁木齐 830052;
2.昌吉农业技术推广中心,新疆昌吉 831100

收稿日期:2020-01-21 出版日期:2020-10-20 发布日期:2020-09-04
通讯作者: 李克梅(1972-),女,江苏人,副教授,研究方向为牧草病害,(E-mail)likemei@xjau.edu.cn
作者简介:窦晓丽(1995-),女,新疆人,硕士生研究生,研究方向为牧草病害,(E-mail)1538866595@qq.com
基金资助:
国家自然科学基金(31760708)。

Biological Characteristics of Pathogens Causing of Two Alfalfa Leaf Spot in Xinjiang

DOU Xiaoli¹, HU Wenjing¹, LIU Fan², LIN Caiying¹, LI Kemei¹

1. College of Agronomy, Xinjiang Agricultural University, Urumqi 830052, China;
2. Changji Agricultural Technology Extension Center, Changji Xinjiang 831100, China

Received:2020-01-21 Online:2020-10-20 Published:2020-09-04
Contact: Li Kemei(1972-), female, native place: Jiangsu, Associate professor, research field: Forage diseases,(E- mail)likemei@xjau.edu.cn
Supported by:
Supported by the Project of the National Natural Science Foundation of China(31760708).

摘要/Abstract

摘要： 【目的】 分析不同培养条件对2种苜蓿叶斑病病原菌苜蓿茎点霉(Phoma medicaginis)和囊状匍柄霉(Stemphylium vesicarium)菌丝生长的影响,研究2种病原菌的生物学特性。【方法】 在不同培养基、温度、碳源、氮源条件下,培养P. medicaginis(代表菌株PM-1)7 d,培养S. vesicarium(代表菌株SV-1)5 d,观察菌落及菌丝生长状况,用十字交叉法分别测量2种病原菌菌落直径大小,采用SPSS 20.0软件分析各处理间的差异。【结果】 菌株PM-1在供试的11种培养基、8种碳源和12种氮源培养基上均可生长,其中最适培养基是GPA和PSA,最佳碳源是麦芽糖,最佳氮源是L-谷氨酰胺,菌落直径在不同培养基、碳源、氮源之间均存在显著差异;在5~30℃均可生长,最适生长温度25℃,致死温度55℃;24 h持续光照有利于菌丝生长。菌株SV-1在供试的11种培养基、8种碳源和12种氮源培养基上均可生长,其中最适培养基是GPA,最佳碳源是菊糖,最佳氮源是L-谷氨酰胺,菌落直径在不同培养基、碳源、氮源之间均存在显著差异;在5~35℃均可生长,最适生长温度25℃,致死温度60℃;不同光照处理对菌丝生长差异不显著。【结论】 苜蓿茎点霉代表菌株PM-1的最适培养条件为温度25℃,GPA或PSA培养基,24 h持续光照,碳、氮源为麦芽糖和L-谷氨酰胺。囊状匍柄霉代表菌株SV-1的最适培养条件为温度25℃,GPA培养基,碳、氮源为菊糖和L-谷氨酰胺。

关键词: :苜蓿茎点霉叶斑病, 苜蓿匍柄霉叶斑病, 苜蓿茎点霉, 囊状匍柄霉, 生物学特性

Abstract: 【Objective】 The effects of two kinds of alfalfa leaf spot pathogens Phoma medicaginis and Stemphylium vesicarium on the mycelial growth biological characteristics of the two strains. 【Method】 Leaf spot fungus P. medicaginis (representing strain PM-1) on different medium, temperature, carbon source, and nitrogen source conditions, were cultured 7 days and 5 dayscolony and mycelium growth during culture. The diameter of colonies was measured by the cross method. Finally, the statistical analysis software SPSS 20.0 was used to analyze the differences between the treatments. 【Result】 PM-1 grow on 11 medium, 8 carbon sources and 12 nitrogen sources. The optimal medium GPA and PSA, the optimal carbon source maltose, and the optimal nitrogen source L-gluten Amine amide. There significant differences between different culture media, carbon sources, sources on the growth of colonies. The temperature that P. medicaginis grow 5℃ to 30℃, while the optimal temperature 25℃; the lethal temperature of mycelium 55℃. The complete illumination was conductive to mycelium growth. SV-1 grow on 11 media, 8 carbon sources, and 12 nitrogen sources. The optimal medium GPA, the optimal carbon source inulin, and the optimal nitrogen source L-gluten Amine amide. There significant differences between different culture media, carbon sources, nitrogen sources on the growth of colonies; The temperature that S. vesicarium grow 5℃ to 35℃, while the optimal temperature 25℃. The lethal temperature of mycelium 60℃; while significant of mycelium growth between different illumination treatments. 【Conclusion】 P. medicaginis (representing strain PM-1) optimal culture conditions were temperature 25℃, GPA or PSA medium, illumination, carbon and nitrogen sources were maltose and L-gluten Amine amidum. S. vesicarium (representing strain SV-1) optimal culture conditions were temperature 25℃, GPA medium, carbon and nitrogen were inulin and L-glutamine.

Key words: alfalfa phoma leaf spot, alfalfa Stemphylium leaf spot, Phoma medicaginis, Stemphylium vesicarium, biological characteristics

中图分类号:

S435.4

窦晓丽, 胡文静, 刘凡, 林彩英, 李克梅. 新疆2种苜蓿叶斑病病原菌主要生物学特性[J]. 新疆农业科学, 0, (): 1854-1861.

DOU Xiaoli, HU Wenjing, LIU Fan, LIN Caiying, LI Kemei. Biological Characteristics of Pathogens Causing of Two Alfalfa Leaf Spot in Xinjiang[J]. Xinjiang Agricultural Sciences, 0, (): 1854-1861.

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新疆2种苜蓿叶斑病病原菌主要生物学特性

Biological Characteristics of Pathogens Causing of Two Alfalfa Leaf Spot in Xinjiang

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