雌激素对奶牛乳腺上皮细胞增殖及细胞周期变化规律的影响

doi:10.6048/j.issn.1001-4330.2019.03.021

新疆农业科学 ›› 2019, Vol. 56 ›› Issue (3): 570-579.DOI: 10.6048/j.issn.1001-4330.2019.03.021

• 植物保护·草业·畜牧兽医·农业经济 • 上一篇下一篇

雌激素对奶牛乳腺上皮细胞增殖及细胞周期变化规律的影响

武开乐¹, 库西塔别克·买买提依不拉音¹, 马静¹, 邵伟^1,2, 余雄²

1.新疆农业大学动物科学学院,乌鲁木齐830052;
2.新疆肉乳用草食动物营养重点试验室,乌鲁木齐830052

收稿日期:2019-02-24 出版日期:2019-03-20 发布日期:2019-06-25
通信作者: 余雄(1958-),男,四川邻水人,教授,博士生导师,研究方向为动物营养与饲料科学,(E-mail) yuxiong8763601@126.com
作者简介:武开乐(1993-),女,新疆人,硕士,研究方向为动物营养与饲料科学,(E-mail) 569883769@qq.com
基金资助:
国家自然科学基金“催乳素对奶山羊移植乳腺干细胞向乳腺组织分化调控机制研究”(3156130247)

Effects of estrogen on the proliferation and cell cycle of mammary gland epithelial cells in dairy cows

Wu Kai-le¹, Kuxitabieke Maimaitiyibulayin¹, Ma Jing¹, Shao Wei^1,2, Yu Xiong²

1.Collage of Animal Science,Xinjiang Agricultural University,Urumqi 830052,China;
2. Xinjiang Key Laboratory of Meat and Milk Production Herbivore Nutrition,Urumqi 830052,China

Received:2019-02-24 Online:2019-03-20 Published:2019-06-25
Correspondence author: Yu Xiong (1958-), male, Sichuan Linshuiren, professor, doctor,research field: animal nutrition and feed science, (E-mail) Yuxiong 8763601@126.com
Supported by:
dy of transplanted mammary stem cells on differentiation and regulation to mammary gland epithelial cells by PRL(3156130247)

摘要/Abstract

摘要： 目的研究雌激素对乳腺上皮细胞增殖及细胞生长周期变化规律的影响。方法选取扩增生长状态良好的乳腺上皮细胞,添加0、50、100、200 μmol/L雌激素孵育乳腺上皮细胞后,分别在0、12、24、48、72 h后检测各组细胞增殖及细胞周期。结果培养12 h时,50 μmoL/L雌激素组的OD值显著高于对照组及100 μmoL/L雌激素组(P＜0.05),且极显著高于200 μmoL/L雌激素添加组(P＜0.01);培养至12 h时,对照组及添加50 μmoL/L雌激素组其G1期极显著高于其他两组(P＜0.01),对照组S期极显著高于其他各组(P＜0.01),添加100 μmoL/L雌激素组其G2期极显著高于其他各组(P＜0.01)。结论添加雌激素可以促进乳腺上皮细胞的增殖,且50 μmoL/L、12 h时雌激素促进乳腺上皮细胞的增殖效果最好。添加雌激素不影响乳腺上皮细胞在各时期时细胞生长的基本规律,各雌激素添加组在12 h时的细胞周期由G1、S期向G2期转化较快。

关键词: 雌激素; 乳腺上皮细胞; 细胞增殖; 细胞周期

Abstract: Objective To investigate the effects of estrogen on the proliferation and cell cycle of mammary epithelial cells. From November 2017 to January 2018, the experiment of estrogen on the proliferation and growth cycle of mammary epithelial cells was carried out in Xinjiang Herbivore Animal Nutrition Laboratory. Method The mammary epithelial cells with good growth state were selected and the mammary epithelial cells were incubated with 0, 50, 100, 200 μmol/L estrogen. The proliferation and cell cycle of each group were detected at 0, 12, 24, 48 and 72 h respectively. Happening. Result The results showed that the OD value of the 50 μmoL/L estrogen group was significantly higher than that of the control group and the 100 μmoL/L estrogen group (P<0.05), and was significantly higher than the 200 μmoL/L estrogen addition group (P<0.01). In the control group and the 50 μmoL/L estrogen group, the G1 phase was significantly higher than the other two groups (P<0.01), and the S phase of the control group was significantly higher than the other groups (P<0.01). The G2 phase of the 100 μmol L/L estrogen group was significantly higher than the other groups (P<0.01). Conclusion The results showed that the addition of estrogen can promote the proliferation of mammary epithelial cells, and estrogen promoted the proliferation of mammary epithelial cells at 50 μmol/L and 12 h. The addition of estrogen did not affect the basic regularity of cell growth in mammary epithelial cells at various stages. The cell cycle of each estrogen-added group changed from G1 to S to G2 at 12 h.

Key words: estrogen; mammary epithelial cells; cell proliferation; cell cycle

中图分类号:

S823S813.3

武开乐, 库西塔别克·买买提依不拉音, 马静, 邵伟, 余雄. 雌激素对奶牛乳腺上皮细胞增殖及细胞周期变化规律的影响[J]. 新疆农业科学, 2019, 56(3): 570-579.

Wu Kai-le, Kuxitabieke Maimaitiyibulayin, Ma Jing, Shao Wei, Yu Xiong. Effects of estrogen on the proliferation and cell cycle of mammary gland epithelial cells in dairy cows[J]. Xinjiang Agricultural Sciences, 2019, 56(3): 570-579.

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雌激素对奶牛乳腺上皮细胞增殖及细胞周期变化规律的影响

Effects of estrogen on the proliferation and cell cycle of mammary gland epithelial cells in dairy cows

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