关键词: 哈萨克羊; MSTN基因; DNA甲基化

Abstract: 【Objective】 To study the DNA methylation level of MSTN (spermatostatin) gene in muscle and adipose tissue, and to provide genetic data for improving the breeding of Kazakh sheep.【Method】The bisulfite treatment sequencing method using whole genome methylation analysis was used to determine the DNA methylation level of MSTN gene of Kazak sheep in CpG dinucleotide acid.【Result】The MSTN gene DNA methylation was low in 6-month-old Kazak sheep muscle, and the highest DNA methylation degree in adipose tissue. The DNA methylation probability of MSTN gene in biceps femoris muscle, triceps femoris muscle, semimembranous muscle, semitendinosus muscle, longissimus dorsi muscle and adipose tissue were as follows: 34.7%, 22.6%, 23.3%, 28%, 42.6%, and 76.7%, respectively.【Conclusion】There is no significant difference in methylation probability of MSTN gene DNA between Kazakh sheep tissues. The methylation probability of fat is the highest, and the methylation probability of muscle tissue is low. The methylation probability of fat is the longest in the back, the methylation probabilities of the femoral second muscle, the trigeminal muscle, the semitendinosus, and the semimembrane muscle are 1.8, 2.2, 3.38, 2.72, and 3.27 times, respectively, followed by adipose tissue ≥ longissimusdorsi muscle ≥ femoral muscle ≥ semitendinosus ≥ semimembranous muscle ≥ trigeminal muscle.

Key words: Kazak sheep; MSTN gene; DNA methylation