不同辣椒品种果实发育过程中DNA甲基化的MSAP分析

doi:10.6048/j.issn.1001-4330.2018.09.004

新疆农业科学 ›› 2018, Vol. 55 ›› Issue (9): 1601-1607.DOI: 10.6048/j.issn.1001-4330.2018.09.004

• 作物遗传育种·种质资源·分子生物学 • 上一篇下一篇

不同辣椒品种果实发育过程中DNA甲基化的MSAP分析

李宁, 帕提古丽·艾斯木托拉, 杨生保, 王柏柯, 唐亚萍, 杨涛, 余庆辉

新疆农业科学院园艺作物研究所,乌鲁木齐 830091

收稿日期:2018-07-27 出版日期:2018-09-20 发布日期:2018-12-07
通信作者: 余庆辉(1970-),男,广东人,研究员,博士,研究方向为加工番茄遗传育种与栽培,(E-mail)yuqinghui98@sina.com
作者简介:李宁(1985-),男,新疆人,助理研究员,硕士,研究方向为蔬菜分子育种与生物信息学,(E-mail)liningbio@163.com
基金资助:
新疆维吾尔自治区自然科学基金项目(2016D01B040)

MSAP Analysis of DNA Methylation in Different Capsicum annuum L.Varieties during Fruit Development

LI Ning, Patiqul Asimutula, YANG Bao-sheng, WANG Bai-ke, TANG Ya-ping, YANG Tao, YU Qing-hui

1.College of Plant Science, Tarim University, Alar Xinjiang 843300, China

Received:2018-07-27 Online:2018-09-20 Published:2018-12-07
Correspondence author: YU Qing-hui(1970-),male,Cantonese, researcher, PhD, research direction the research on genetic breeding and cultivation of processed tomatoes. (E-mail)yuqinghui98@sina.com
Supported by:
The Natural Science Foundation of Xinjiang Uyghur Autonomous Region of China (2016D01B040)

摘要/Abstract

摘要： 【目的】分析辣椒GB14和GB38果实发育过程中酶切位点的甲基化,为在分子水平上研究辣椒红素调控规律、在辣椒遗传育种中奠定理论基础。【方法】对辣椒果实发育成熟过程中的DNA甲基化修饰进行分析。【结果】DNA半甲基化条带比率与全甲基化条带比率均随着籽粒的生长发育而逐渐升高,两种辣椒果实绿熟期和红熟期基因组总体甲基化比例分别为36.51%、36.38%、35.57%和35.45%。对甲基化表现呈多态性变化的8个片段回收、测序分析表明,DNA甲基化发生的位点既存在于基因组的编码区,也存在于非编码区。【结论】在辣椒果实发育过程中,DNA甲基化修饰发生了巨大的变化;CCGG位点的半甲基化水平与辣椒红素积累呈正比。

关键词: 辣椒; 果实发育; DNA甲基化; 甲基化敏感扩增多态性分析

Abstract: 【Objective】 This project aims to carry out methylation analysis of restriction endonuclease sites in GB14 and GB38 fruit development of capsicum in the hope of providing a new insight for exploring the molecular mechanism of capsicum fruit development.【Method】The modification of DNA methylation during the development and maturation of Capsicum annuum L. fruit was studied. 【Result】The data showed that the ratios of DNA methylation and total methylation bands both increased gradually with seeds growth and development. The total methylation ratio of the two varieties of Capsicum annuum L.in green maturity period and red ripening period were 36.51%, 36.38%, 35.57% and 35.45%, respectively. Eight polymorphic fragments of methylation showed that the DNA methylation sites existed both in the coding region of the genome and in the non-coding region by the recovery and sequencing.【Conclusion】During the development of capsicum fruit, the modification of DNA methylation was greatly changed and the level of semi-methylation of CCGG loci was positively related to the accumulation of Capsicum annuum L. These results suggest a molecular level method for further studying the regulation of Capsicum annuum L., which provide a theoretical foundation for the breeding of Capsicum annuum L.

Key words: Capsicum annuum L.; fruit development; DNA methylation; MSAP analysis ; ;

中图分类号:

S641.3
S188

李宁, 帕提古丽·艾斯木托拉, 杨生保, 王柏柯, 唐亚萍, 杨涛, 余庆辉. 不同辣椒品种果实发育过程中DNA甲基化的MSAP分析[J]. 新疆农业科学, 2018, 55(9): 1601-1607.

LI Ning, Patiqul Asimutula, YANG Bao-sheng, WANG Bai-ke, TANG Ya-ping, YANG Tao, YU Qing-hui. MSAP Analysis of DNA Methylation in Different Capsicum annuum L.Varieties during Fruit Development[J]. Xinjiang Agricultural Sciences, 2018, 55(9): 1601-1607.

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不同辣椒品种果实发育过程中DNA甲基化的MSAP分析

MSAP Analysis of DNA Methylation in Different Capsicum annuum L.Varieties during Fruit Development

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