Extraction of β-carotene and antioxidant activity of Lycium barbarum dander by stochastic center-of-mass mapping optimization method

doi:10.6048/j.issn.1001-4330.2025.07.024

Xinjiang Agricultural Sciences ›› 2025, Vol. 62 ›› Issue (7): 1794-1802.DOI: 10.6048/j.issn.1001-4330.2025.07.024

• Fruits and vegetables and processing column • Previous Articles Next Articles

Extraction of β-carotene and antioxidant activity of Lycium barbarum dander by stochastic center-of-mass mapping optimization method

LIANG Lixian¹^,²(), MENG Xintao¹^,³(), LIU Yiming¹^,², ZHANG Ting¹^,³, MA Yan¹^,³, XU Mingqiang¹^,³, PAN Yan¹^,³()

1. Research Institute of Agro-product Storage and Processing, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China
2. College of Food Sciences and Pharmacy, Xinjiang Agricultural University, Urumqi 830091, China
3. Xinjiang Engineering and Technology Research Center for Intensive Processing of Major Agricultural By-products, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China

Received:2024-12-07 Online:2025-07-20 Published:2025-09-05
Correspondence author: MENG Xintao, PAN Yan
Supported by:
Key R & D Program Project of Xinjiang Uygur Autonomous Region(2023B02009-1);Basic Scientific R &D Program of Public Welfare Research Institutions of Xinjiang Uygur Autonomous Region(KY2022013)

随机质心映射优化法提取枸杞皮渣β-胡萝卜素及抗氧化活性分析

梁丽鲜¹^,²(), 孟新涛¹^,³(), 刘一鸣¹^,², 张婷¹^,³, 马燕¹^,³, 许铭强¹^,³, 潘俨¹^,³()

1.新疆维吾尔自治区农业科学院农产品加工研究所, 乌鲁木齐 830091
2.新疆农业大学食品科学与药学学院, 乌鲁木齐 830091
3.新疆主要农副产品精深加工工程技术研究中心, 乌鲁木齐 830091

通讯作者: 孟新涛,潘俨
作者简介:梁丽鲜(2000-),女,硕士研究生,研究方向为农产品贮运与加工,(E-mail)2845887274@qq.com
基金资助:
新疆维吾尔自治区重点研发项目(2023B02009-1);新疆维吾尔自治区公益性科研院所基本科研业务费专项(KY2022013)

Abstract

Abstract:

【Objective】 The aim of this study is to optimize the better extraction process of β-carotene from the skin residue of Xinjiang Lycium barbarum in order to promote the comprehensive utilization of Xinjiang Lycium barbarum resources.Provides theoretical basis and technical support for the high-value utilization of Lycium barbarum dregs. 【Methods】 Anhydrous ethanol was used as the extraction solvent, and the effects of material-liquid ratio, temperature, time, and the number of extraction times on the extraction amount of β-carotene from the skin dregs of Lycium barbarum were investigated. The extraction process was optimized by Random Centroid Optimization (RCO), and then the better extraction conditions were determined. In addition, the antioxidant capacity of β-carotene against DPPH radicals and ABTS radicals was also determined. 【Results】 The optimized parameters of the best extraction process were: material-liquid ratio 1∶16 g/mL, temperature 48℃, time 41 min, and number of extractions twice. Under these conditions, the extracted amount of β-carotene reached 0.53 mg/g. β-carotene showed good antioxidant ability, and the scavenging rates of DPPH and ABTS radicals were 60.89% and 66.11% at a mass concentration of 1.0 mg/mL, respectively. 【Conclusion】

Key words: Stochastic center-of-mass mapping optimization (RCO) method; Lycium barbarum dander; β-carotene extraction; antioxidant activity

摘要：

【目的】研究优化新疆枸杞皮渣中β-胡萝卜素的较佳提取工艺,促进新疆枸杞资源的综合利用,为枸杞皮渣的高值化利用提供理论依据和技术支持。【方法】采用无水乙醇作为提取溶剂,考察料液比、温度、时间、提取次数对枸杞皮渣β-胡萝卜素提取量的影响。通过随机质心映射法(Random Centroid Optimization, RCO)优化提取工艺,确定较佳提取条件。并测定β-胡萝卜素对DPPH自由基和ABTS自由基的抗氧化能力。【结果】优化后的最佳提取工艺参数为料液比1∶16 g/mL、温度48℃、时间41 min、提取次数2次。在此条件下,β-胡萝卜素的提取量达到0.53 mg/g。β-胡萝卜素表现出良好的抗氧化能力,在质量浓度为1.0 mg/mL时,对DPPH和ABTS自由基的清除率分别为60.89%和66.11%。【结论】优化了枸杞皮渣中β-胡萝卜素的提取工艺。最佳提取条件为料液比1∶17 mg/mL、温度57℃、时间45 min、提取次数2次,提取量为0.53 μg/g。

关键词: 随机质心映射优化法(RCO), 枸杞皮渣, β-胡萝卜素提取, 抗氧化活性

CLC Number:

S663.9

LIANG Lixian, MENG Xintao, LIU Yiming, ZHANG Ting, MA Yan, XU Mingqiang, PAN Yan. Extraction of β-carotene and antioxidant activity of Lycium barbarum dander by stochastic center-of-mass mapping optimization method[J]. Xinjiang Agricultural Sciences, 2025, 62(7): 1794-1802.

梁丽鲜, 孟新涛, 刘一鸣, 张婷, 马燕, 许铭强, 潘俨. 随机质心映射优化法提取枸杞皮渣β-胡萝卜素及抗氧化活性分析[J]. 新疆农业科学, 2025, 62(7): 1794-1802.

Figures/Tables 8

References 30

[1]	Yu J, Yan Y M, Zhang L T, et al. A comprehensive review of goji berry processing and utilization[J]. Food Science & Nutrition, 2023, 11(12): 7445-7457.
[2]	纪梦衫, 魏文君, 张凯笑, 等. 枸杞中类胡萝卜素类成分的研究进展[J]. 华西药学杂志, 2024, 39(1): 99-102.
	JI Mengshan, WEI Wenjun, ZHANG Kaixiao, et al. Research progress on carotenoids in Lycium barbarum[J]. West China Journal of Pharmaceutical Sciences, 2024, 39(1): 99-102.
[3]	马晓燕. 枸杞籽油的超临界萃取及其微胶囊化技术的研究[D]. 济南: 齐鲁工业大学, 2014.
	MA Xiaoyan. Research on supercritical extraction of wolfberry seed oil and its microencapsulation technology[D]. Jinan: Qilu University of Technology, 2014.
[4]	李冠杰, 苗侨伟, 王晓丽, 等. 枸杞渣的营养成分及在畜禽饲料中的应用[J]. 广东饲料, 2023, 32(7): 38-40.
	LI Guanjie, MIAO Qiaowei, WANG Xiaoli, et al. Nutrient composition of matrimony vine berry residue and its application in animal feed[J]. Guangdong Feed, 2023, 32(7): 38-40.
[5]	张惠玲. 枸杞的综合开发与利用[J]. 食品研究与开发, 2012, 33(2): 223-227.
	ZHANG Huiling. Barbary wolfberry fruit efficacy and comprehensive utilization[J]. Food Research and Development, 2012, 33(2): 223-227.
[6]	朱培蕾. 乳清分离蛋白复合大米淀粉的干热改性及其稳定β-胡萝卜素乳液凝胶的研究[D]. 合肥: 安徽农业大学, 2021.
	ZHU Peilei. Study on dry-heat modification of Whey Protein Isolation compound rice starch and its stability of β-carotene emulsion gel[D]. Hefei: Anhui Agricultural University, 2021.
[7]	Conidi C, Drioli E, Cassano A. Coupling ultrafiltration-based processes to concentrate phenolic compounds from aqueous goji berry extracts[J]. Molecules, 2020, 25(16): 3761.
[8]	范晓岚, 杨军, 糜漫天, 等. β-胡萝卜素的抗氧化作用与疾病预防[J]. 中国公共卫生, 2003, 19(4): 479-480.
	FAN Xiaolan, YANG Jun, MI Mantian, et al. Antioxidant effect of β-carotene and disease prevention[J]. China Public Health, 2003, 19(4): 479-480.
[9]	任冰倩, 汪锋, 陆姝欢, 等. β-胡萝卜素生产、提取及功能的研究进展[J]. 山东化工, 2023, 52(14): 69-72.
	REN Bingqian, WANG Feng, LU Shuhuan, et al. Research progress on production, extraction technology and functions of β-carotene[J]. Shandong Chemical Industry, 2023, 52(14): 69-72.
[10]	Demesa A G, Saavala S, Pöysä M, et al. Overview and toxicity assessment of ultrasound-assisted extraction of natural ingredients from plants[J]. Foods, 2024, 13(19): 3066.
[11]	陈林茂. 新鲜螺旋藻中β-胡萝卜素提取工艺[J]. 中国食品工业, 2023(21): 94-97.
	CHEN Linmao. Extraction technology of β-carotene from fresh Spirulina[J]. China Food Industry, 2023(21): 94-97.
[12]	Shahram H, Dinani S T. Optimization of ultrasonic-assisted enzymatic extraction of β-carotene from orange processing waste[J]. Journal of Food Process Engineering, 2019, 42(4): e13042.
[13]	孟新涛, 潘俨, 许铭强, 等. 随机质心映射优化法(RCO)提取皮亚曼石榴皮中黄酮类化合物[J]. 食品工业科技, 2016, 37(2): 276-280, 286.
	MENG Xintao, PAN Yan, XU Mingqiang, et al. Random centroid optimization of flavonoids extraction from Pierman pomegranate[J]. Science and Technology of Food Industry, 2016, 37(2): 276-280, 286.
[14]	Bao J, Zhang X, Zheng J H, et al. Mixed fermentation of Spirulina platensis with Lactobacillus plantarum and Bacillus subtilis by random-centroid optimization[J]. Food Chemistry, 2018, 264: 64-72.
[15]	Dou J L, Toma S, Nakai S. Random-centroid optimization for food formulation[J]. Food Research International, 1993, 26(1): 27-37.
[16]	Ma Y, Lian Y L, Meng X T, et al. Process optimization of sea buckthorn fruit powder effervescent tablets by random centroid methodology combined with fuzzy mathematical sensory evaluation[J]. Processes, 2023, 11(9): 2639.
[17]	沈棚, 黄敏欣, 白卫东, 等. 随机质心衍射优化酶法去除客家娘酒中氨基甲酸乙酯工艺[J]. 食品科学, 2013, 34(22): 40-43. DOI
	SHEN Peng, HUANG Minxin, BAI Weidong, et al. Optimization of enzymatic removal of urethane from Hakka mother wine using random centroid optimization methodology[J]. Food Science, 2013, 34(22): 40-43. DOI
[18]	Ogawa M, Nakamura S, Scaman C H, et al. Enhancement of proteinase inhibitory activity of recombinant human cystatin C using random-centroid optimization[J]. Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics, 2002, 1599(1/2): 115-124.
[19]	Li Y, Fabiano-Tixier A S, Tomao V, et al. Green ultrasound-assisted extraction of carotenoids based on the bio-refinery concept using sunflower oil as an alternative solvent[J]. Ultrasonics Sonochemistry, 2013, 20(1): 12-18. DOI PMID
[20]	向珊珊, 张楠, 潘晴, 等. 木鳖果假种皮中β-胡萝卜素的提取工艺优化及稳定性研究[J]. 食品工业科技, 2018, 39(21): 191-195, 201.
	XIANG Shanshan, ZHANG Nan, PAN Qing, et al. Extraction technology optimization and stability ofβ-carotene from Momordica cochinchinensis aril[J]. Science and Technology of Food Industry, 2018, 39(21): 191-195, 201.
[21]	赵明德. 不同方法提取胡萝卜中类胡萝卜素工艺研究[J]. 青海师范大学学报(自然科学版), 2021, 37(4): 53-58.
	ZHAO Mingde. Different methods to extract carotenoids in carrots technology research[J]. Journal of Qinghai Normal University (Natural Science), 2021, 37(4): 53-58.
[22]	翟丹云, 高晓辉, 陈庆安, 等. 籽用南瓜中β-胡萝卜素的提取技术研究[J]. 食品研究与开发, 2012, 33(12): 57-60.
	ZHAI Danyun, GAO Xiaohui, CHEN Qingan, et al. Study on the extraction technology of β-carotene from seed-used pumpkin[J]. Food Research and Development, 2012, 33(12): 57-60.
[23]	马丽媛, 石光蕊, 尚尔坤, 等. 超声辅助提取玉米黄色素及其抗氧化活性研究[J]. 发酵科技通讯, 2023, 52(2): 63-68.
	MA Liyuan, SHI Guangrui, SHANG Erkun, et al. Study on ultrasound-assisted extraction of corn yellow pigment and its antioxidant activity[J]. Bulletin of Fermentation Science and Technology, 2023, 52(2): 63-68.
[24]	王玉洁, 钟俣蕃, 陈正喜, 等. 油樟叶多糖的提取工艺优化及其抗氧化活性分析[J/OL]. 宜宾学院学报, 2024: 1-16. (2024-11-28). https://kns.cnki.net/KCMS/detail/detail.aspxfilename=YBSG20241126001&dbname=CJFD&dbcode=CJFQ.
	WANG Yujie, ZHONG Yufan, CHEN Zhengxi, et al. Optimization of extraction technology and analysis of antioxidant activity of polysaccharide from Cinnamomum camphora leaves[J/OL]. China Industrial Economics, 2024: 1-16. (2024-11-28). https://kns.cnki.net/KCMS/detail/detail.aspxfilename=YBSG20241126001&dbname=CJFD&dbcode=CJFQ.
[25]	刘垠泽, 慕雪, 刘德江, 等. 碱提法提取升麻渣多糖工艺优化及抗氧化活性分析[J]. 南方农业学报, 2024, 55(7): 2021-2030.
	LIU Yinze, MU Xue, LIU Dejiang, et al. Process optimization and antioxidant activity of polysaccharide from Actaea cimicifuga L. residue extracted by alkaline method[J]. Journal of Southern Agriculture, 2024, 55(7): 2021-2030.
[26]	Baliyan S, Mukherjee R, Priyadarshini A, et al. Determination of antioxidants by DPPH radical scavenging activity and quantitative phytochemical analysis of Ficus religiosa[J]. Molecules, 2022, 27(4): 1326.
[27]	Fernandes P A R, Coimbra M A. The antioxidant activity of polysaccharides: a structure-function relationship overview[J]. Carbohydrate Polymers, 2023, 314: 120965.
[28]	曹静亚, 谭亮, 迟晓峰, 等. 枸杞子中β-胡萝卜素的快速溶剂萃取提取条件优化及HPLC测定[J]. 中药材, 2013, 36(7): 1168-1171.
	CAO Jingya, TAN Liang, CHI Xiaofeng, et al. Optimization of extraction conditions of β-carotene from Lycium barbarum L. by rapid solvent extraction and determination by HPLC[J]. Journal of Chinese Medicinal Materials, 2013, 36(7): 1168-1171.
[29]	涂凯峰, 余修亮, 潘瑶, 等. 聚能超声波辅助提取胡萝卜中β-胡萝卜素的提取工艺[J]. 食品工业, 2017, 38(12): 91-97.
	TU Kaifeng, YU Xiuliang, PAN Yao, et al. Ultrasonic-assisted extraction technology of beta carotene from carrots[J]. The Food Industry, 2017, 38(12): 91-97.
[30]	孙波, 黄昊, 王娟, 等. 不同产地枸杞中β-胡萝卜素的含量及其抗氧化活性研究[J]. 时珍国医国药, 2012, 23(7): 1666-1667.
	SUN Bo, HUANG Hao, WANG Juan, et al. Content determination and antioxidant activity of β-carotene in Lycium barbarum L. from different regions[J]. Lishizhen Medicine and Materia Medica Research, 2012, 23(7): 1666-1667.

循环次数 Cycles	待优化因子 Factor to be optimized	料液比 Material- liquid ratio (g/mL)	提取温度 Extraction temperature (℃)	提取时间 Extraction time(min)	提取次数 Extraction times
第一次随机质心搜索 The first random centroid search	因子上限	1∶5	25	20	1
第一次随机质心搜索 The first random centroid search	因子下限	1∶35	65	60	3
第一次随机质心搜索 The first random centroid search	因子上限	1∶17	55	38	1
第一次随机质心搜索 The first random centroid search	因子下限	1∶27	65	47	3

循环次数 Cycles	待优化因子 Factor to be optimized	料液比 Material- liquid ratio (g/mL)	提取温度 Extraction temperature (℃)	提取时间 Extraction time(min)	提取次数 Extraction times
第一次随机质心搜索 The first random centroid search	因子上限	1∶5	25	20	1
第一次随机质心搜索 The first random centroid search	因子下限	1∶35	65	60	3
第一次随机质心搜索 The first random centroid search	因子上限	1∶17	55	38	1
第一次随机质心搜索 The first random centroid search	因子下限	1∶27	65	47	3

循环次数 Number of cycles	试验编号 Experiment No.	料液比 Material- liquid ratio (g/mL)	提取温度 Extraction temperature (℃)	提取时间 Extraction time (min)	提取次数 Number of extractions	提取量 Extracted amount (mg /g)
第一轮循环随机搜索试验 First round of cyclic randomized search experiments	1	1∶15	44	51	2	0.22
	2	1∶23	32	21	1	0.46
	3	1∶22	58	51	2	0.39
	4	1∶31	60	41	2	0.23
	5	1∶34	36	22	2	0.12
	6	1∶5	57	39	2	0.29
	7	1∶30	61	38	3	0.18
	8	1∶15	32	60	1	0.14
	9	1∶24	40	45	2	0.11
第一次质心搜索 First center of gravity search	10	1∶20	52	38	2	0.43
	11	1∶16	48	41	2	0.52
	12	1∶23	49	41	2	0.46
	13	1∶19	48	38	2	0.24

循环次数 Number of cycles	试验编号 Experiment No.	料液比 Material- liquid ratio (g/mL)	提取温度 Extraction temperature (℃)	提取时间 Extraction time (min)	提取次数 Number of extractions	提取量 Extracted amount (mg /g)
第一轮循环随机搜索试验 First round of cyclic randomized search experiments	1	1∶15	44	51	2	0.22
	2	1∶23	32	21	1	0.46
	3	1∶22	58	51	2	0.39
	4	1∶31	60	41	2	0.23
	5	1∶34	36	22	2	0.12
	6	1∶5	57	39	2	0.29
	7	1∶30	61	38	3	0.18
	8	1∶15	32	60	1	0.14
	9	1∶24	40	45	2	0.11
第一次质心搜索 First center of gravity search	10	1∶20	52	38	2	0.43
	11	1∶16	48	41	2	0.52
	12	1∶23	49	41	2	0.46
	13	1∶19	48	38	2	0.24

Extraction of β-carotene and antioxidant activity of Lycium barbarum dander by stochastic center-of-mass mapping optimization method

随机质心映射优化法提取枸杞皮渣β-胡萝卜素及抗氧化活性分析

RichHTML

PDF

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 8

References 30

Related Articles 4

Recommended Articles

Metrics

循环次数 Number of cycles	试验编号 Experiment No.	料液比 Material-liquid ratio(g/mL)	提取温度 Extraction temperature(℃)	提取时间 Extraction time(min)	提取次数 Number of extractions	提取量 Extracted amount(mg /g)
第二轮循环随机搜索试验 Second round of cyclic randomized search experiments	14	1∶24	63	39	3	0.23
	15	1∶21	57	46	1	0.43
	16	1∶21	56	42	2	0.41
	17	1∶18	64	43	2	0.32
	18	1∶26	60	46	2	0.21
	19	1∶21	61	42	1	0.35
	20	1∶25	61	43	2	0.44
第二次质心搜索 Second center of mass search	21	1∶20	57	46	2	0.41
	22	1∶16	57	43	2	0.28
	23	1∶17	57	44	2	0.51
	24	1∶17	57	45	2	0.53

[1]	Zulipiya Maimaiti, MAO Hongyan, YUE Li, WANG Jiamin, YU Ming. Study on in vitro antioxidant activities and digestive characteristics of tricolor quinoa [J]. Xinjiang Agricultural Sciences, 2023, 60(10): 2461-2469.
[2]	HAN Haixia, YOU Lin, ZHONG Zhiming, WANG Na, LI Shuang. Study on the Effects of Different Temperatures on Phenylethanol Glycosides duringthe Preparation of Cistanche deserticola Extractum and the Antioxidant Activities of the Extractum [J]. Xinjiang Agricultural Sciences, 2022, 59(8): 1975-1983.
[3]	DUAN Jing-feng;WANG Yan-xia;LI Xiao-yuan;HUANG Jun-hai;BAI Hong-jin. Diversity of Polysaccharide Content of Five Zizphus jujube in Alar Area and Their in Vitro Antioxidative Activities [J]. , 2014, 51(5): 831-838.
[4]	. Study on Antioxidant Activity of Arnebia euchroma (Royle) Johnst Hairy Roots [J]. , 2010, 47(2): 291-295.