Analysis of expression patterns of glycometabolic pathway genes in sorghum anti-aphid response

doi:10.6048/j.issn.1001-4330.2025.05.022

Abstract

Abstract:

【Objective】 Analysis of expression patterns of glycometabolic pathway genes in sorghum anti-aphid response.【Methods】 The aphid resistant variety HN16 and its sensitive aphid mutantasm1 were used as research materials and the transcriptomic database of sorghum was used to screen the differentially expressed genes (DEGs) of the two materials in the feeding process of aphids. In the end, a total of 17 genes were screened. These results suggested that feeding by aphids changed the expression of glycometabolic pathway genes in HN16 and asm1. Six pairs of specific primers of six genes (sbGOLS2, sbGUX2, sbCHIT3, sbBGL22, sbISOA3) were selected by PCR and qPCR for gene expression verification and analysis.【Results】 The results showed that the expression patterns of these 6 genes were basically consistent with the transcriptome data, and they played different roles in the anti-insect response of HN16. Among them, the genessbGOLS2, sbCHIT3 andsbBGL22 were resistant to insects through rapid response to avoid excessive energy consumption. sbGUAA2, sbGUX2 andsbISOA3 ensured the stability of glycometabolic metabolism of sorghum by maintaining relatively stable expression during aphid feeding. 【Conclusion】 In summary, during the feeding process of aphids, HN16 maintains a relatively stable glycometabolic metabolism level by regulating the expression of glycometabolic metabolism pathway genes to resist the harm of aphids.

Key words: sorghum; sorghum aphid; insect resistance; glycometabolism

摘要：

【目的】高粱抗蚜反应中糖代谢途径基因的表达模式分析。【方法】以高粱抗蚜品种HN16及其感蚜突变体asm1作为材料,利用高粱转录组数据库中筛选2材料在蚜虫取食过程中的糖代谢途径差异表达基因 (DEGs),共筛选到17个基因,蚜虫的取食改变了HN16和asm1中糖代谢途径基因的表达。利用PCR及荧光定量PCR,筛选出6个基因 (sbGOLS2、sbGUX2、sbCHIT3、sbBGL22、sbISOA3) 的6对特异性引物用于基因表达量验证及分析。【结果】6个基因的表达量变化规律与转录组数据基本吻合,且在HN16的抗虫反应中作用有所不同,其中基因sbGOLS2、sbCHIT3和sbBGL22通过快速反应来抵抗昆虫,避免消耗过多的能量;而sbGUAA2、sbGUX2和sbISOA3,在蚜虫取食过程中通过维持较为稳定的表达,保证高粱糖代谢的稳定性。【结论】蚜虫取食过程中,HN16通过调节糖代谢途径基因的表达,维持体内较为稳定的糖代谢水平,以抵御蚜虫为害。

关键词: 高粱, 高粱蚜, 抗虫性, 糖代谢

CLC Number:

S514

HAO Le, LIU Yuan, HAN Ruxue, WANG Zhenjie, SHAO Yutao. Analysis of expression patterns of glycometabolic pathway genes in sorghum anti-aphid response[J]. Xinjiang Agricultural Sciences, 2025, 62(5): 1242-1248.

郝乐, 刘媛, 韩茹雪, 张鹏, 王振杰, 邵玉涛. 高粱抗蚜反应中糖代谢途径基因的表达模式分析[J]. 新疆农业科学, 2025, 62(5): 1242-1248.

Figures/Tables 7

References 20

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试剂 Reagent	体系 System(μL)
Total RNA/mRNA	1
Anchored Oligo (dT) primer	1
2×TS Reaction Mix	10
TransScriptRT/RI Enzyme Mix	1
gDNA Remover	1
RNase-free Water	6

试剂 Reagent	体系 System(μL)
Total RNA/mRNA	1
Anchored Oligo (dT) primer	1
2×TS Reaction Mix	10
TransScriptRT/RI Enzyme Mix	1
gDNA Remover	1
RNase-free Water	6

基因名称 Gene name	简称 Abbreviation	描述 Description
Sobic.002G423600	sbGOLS2	半乳糖醇合酶 Galactinol synthase 2
Sobic.001G479800	sbGUX2	UDP葡糖醛酸:木聚糖-α-葡糖醛酸基转移酶2 UDP-glucuronate: xylan alpha-glucuronosyltransferase 2
Sobic.001G138200	sbGATL1	半乳糖醛糖基转移酶1 Probable galacturonosyltransferase-like 1
Sobic.004G244100	sbGATL9	半乳糖醛糖基转移酶9 Probable galacturonosyltransferase-like 9
oSobic.002G268200	sbFUCO1	α-L-岩藻糖苷酶 Alpha-L-fucosidase 1
Sobic.004G004800	sbSST	蔗糖:蔗糖1-果糖基转移酶 Sucrose: sucrose 1-fructosyltransferase
Sobic.001G263500	sbE1311	葡聚糖内切-1,3-葡糖苷酶11 Glucan endo-1,3-beta-glucosidase 11
Sobic.003G364600	sbE1314	葡聚糖内切-1,3-葡糖苷酶14 Glucan endo-1,3-beta-glucosidase 14
Sobic.001G441700	sbXTH27	木葡聚糖内转葡糖基酶/水解酶蛋白27 Probable xyloglucan endotransglucosylase/hydrolase protein 27
Sobic.004G273200	sbXTH30	木葡聚糖内转葡糖基酶/水解酶蛋白30 Probable xyloglucan endotransglucosylase/hydrolase protein 30
Sobic.003G244600	sbCHIT3	酸性内切几丁质酶 Acidic endochitinase
Sobic.004G042700	sbGUN5	内切葡聚糖酶5 Endoglucanase 5
Sobic.003G325400	sbGUAA1	GMP合成酶〔谷氨酰胺水解酶〕 Probable GMP synthase [glutamine-hydrolyzing]
Sobic.007G223600	sbGUAA2	GMP合成酶〔谷氨酰胺水解酶〕 Probable GMP synthase [glutamine-hydrolyzing]
Sobic.006G132700	sbCHIA	内切几丁质酶 Endochitinase A
Sobic.009G114000	sbBGL22	β-葡萄糖苷酶22 Beta-glucosidase 22
Sobic.002G233600	sbISOA3	异淀粉酶3,叶绿体 Isoamylase 3, chloroplastic

基因名称 Gene name	简称 Abbreviation	描述 Description
Sobic.002G423600	sbGOLS2	半乳糖醇合酶 Galactinol synthase 2
Sobic.001G479800	sbGUX2	UDP葡糖醛酸:木聚糖-α-葡糖醛酸基转移酶2 UDP-glucuronate: xylan alpha-glucuronosyltransferase 2
Sobic.001G138200	sbGATL1	半乳糖醛糖基转移酶1 Probable galacturonosyltransferase-like 1
Sobic.004G244100	sbGATL9	半乳糖醛糖基转移酶9 Probable galacturonosyltransferase-like 9
oSobic.002G268200	sbFUCO1	α-L-岩藻糖苷酶 Alpha-L-fucosidase 1
Sobic.004G004800	sbSST	蔗糖:蔗糖1-果糖基转移酶 Sucrose: sucrose 1-fructosyltransferase
Sobic.001G263500	sbE1311	葡聚糖内切-1,3-葡糖苷酶11 Glucan endo-1,3-beta-glucosidase 11
Sobic.003G364600	sbE1314	葡聚糖内切-1,3-葡糖苷酶14 Glucan endo-1,3-beta-glucosidase 14
Sobic.001G441700	sbXTH27	木葡聚糖内转葡糖基酶/水解酶蛋白27 Probable xyloglucan endotransglucosylase/hydrolase protein 27
Sobic.004G273200	sbXTH30	木葡聚糖内转葡糖基酶/水解酶蛋白30 Probable xyloglucan endotransglucosylase/hydrolase protein 30
Sobic.003G244600	sbCHIT3	酸性内切几丁质酶 Acidic endochitinase
Sobic.004G042700	sbGUN5	内切葡聚糖酶5 Endoglucanase 5
Sobic.003G325400	sbGUAA1	GMP合成酶〔谷氨酰胺水解酶〕 Probable GMP synthase [glutamine-hydrolyzing]
Sobic.007G223600	sbGUAA2	GMP合成酶〔谷氨酰胺水解酶〕 Probable GMP synthase [glutamine-hydrolyzing]
Sobic.006G132700	sbCHIA	内切几丁质酶 Endochitinase A
Sobic.009G114000	sbBGL22	β-葡萄糖苷酶22 Beta-glucosidase 22
Sobic.002G233600	sbISOA3	异淀粉酶3,叶绿体 Isoamylase 3, chloroplastic

基因简称 Gene abbreviation	正向引物 (5'-3) Forward primer (5'-3')	反向引物 (5'-3) Reverse primer (5'-3')	片段长度 Fragment length
sbGOLS2	CTCAACGAGGCGCTTAATTATT	CAATTCCAAAACCTGTAGACCG	84
sbGUX2	TGATGATGATCGACTACGTACC	CTAGCTATCTCGCTCTCAGCTA	80
sbCHIT3	CTTCTACGATGTGCAGAACAAC	CTTGACGAATGAATAGCCACTG	109
sbGUAA2	ATTCATAGTCTGCCTCCCAAAA	TAGATCCTCTGCATGTACTTGC	193
sbBGL22	ATCAGCTTATCAGTACGAAGGG	CTTGTATTTGTGGTAGCCGTCT	136
sbISOA3	CAGCATAAATCTTGGAGTCGTG	GGAAGTTACTTGTGTTTGCCAT	223