Optimization of experimental conditions for the treatment of potato starch wastewater by coupling of acidophilic photosynthetic bacteria and Bacillus atrophaeus

doi:10.6048/j.issn.1001-4330.2023.11.023

Xinjiang Agricultural Sciences ›› 2023, Vol. 60 ›› Issue (11): 2798-2805.DOI: 10.6048/j.issn.1001-4330.2023.11.023

• Microbes · Horticultural Special Local Products·Agricultural Product Processing Engineering • Previous Articles Next Articles

Optimization of experimental conditions for the treatment of potato starch wastewater by coupling of acidophilic photosynthetic bacteria and Bacillus atrophaeus

ZENG Jun¹(), WU Lei², GAO Yan¹(), YANG Hongmei¹, LIN Qing¹, HUO Xiangdong¹

1. Xinjiang Laboratory of Special Environmental Microbiology/ Institute of Applied Microbiology, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China
2. Karamay Ruiheng Animal Husbandry Development Co., Ltd., Karamay Xinjiang 834000, China

Received:2023-03-11 Online:2023-11-20 Published:2023-12-07
Correspondence author: GAO Yan(1979-),female,Jiangyin,Jiangsu Province,researcher,research field:resources and applied microbiology(E-mail)47795411@qq.com
Supported by:
Key Laboratory of Xinjiang Autonomous Region(2019D04016);The Key Science and Technology Innovation Incubation Project of Xinjiang Academy of Agricultural Sciences(xjkcpy-2020005)

嗜酸光合细菌耦合萎缩芽孢杆菌降解马铃薯加工淀粉废水条件优化

曾军¹(), 武磊², 高雁¹(), 杨红梅¹, 林青¹, 霍向东¹

1.新疆农业科学院微生物应用研究所/新疆特殊环境微生物实验室,乌鲁木齐 830091
2.克拉玛依瑞恒畜牧开发有限责任公司,新疆克拉玛依 834000

通讯作者: 高雁(1979-),女,江苏江阴人,研究员,博士,硕士生导师,研究方向为资源与应用微生物学,(E-mail) 47795411@qq.com
作者简介:曾军(1982-),男,新疆玛纳斯人,副研究员,研究方向为特殊环境光合细菌功能及生态学,(E-mail)leo924.student@sina.com
基金资助:
新疆维吾尔自治区重点实验室项目(2019D04016);新疆农业科学院科技创新重点培育专项(xjkcpy-2020005)

Abstract

Abstract:

【Objective】 This project aims to optimize the experimental conditions for the treatment of potato starch wastewater by coupling of Acidophilic photosynthetic bacteria (PSB) and Bacillus atrophaeus (BA). 【Methods】 Potato starch wastewater was used as study materials and they were divided into treatment and control groups. The treatment groups were the compounding ratio, inoculation, culture methods of the two strains and the wastewater contaminants degradation (like COD and Malodorous gas) rates were used to reflect their effects. Finally, we tested the total application effect of the optimized experimental conditions for the treatment of potato starch wastewater. 【Results】 The optimized experimental conditions for the treatment of potato starch wastewater were∶ the ratio of PSB to BA =10∶1, 15% of inoculation, 30℃ of incubation, and incubated 144 h including light intensity 5,000 lx under anaerobic illumination for the first 72 h,then the next 72 h incubated under anaerobic illumination/aerobic =12 h∶12 h. The COD degradation rate under the optimized condition was increased from 16.9% in control group (without inoculation) to 98.21%, and it also showed more than 98% of degradation rate of other contaminates (like ammonia nitrogen, nitrate nitrogen, total phosphorus, mercaptan, hydrogen sulfide) and its self-flocculating rate was 8 cm/h.【Conclusion】 The results suggested that the wastewater contaminated with high degradation rate by coupling of acidophilic photosynthetic bacteria and Bacillus atrophaeus is mainly attributed to the niche differentiation of the optimal growth pH.

Key words: acidophilic photosynthetic bacteria; Bacillus atrophaeus; potato starch wastewater; coupling mechanism

摘要：

【目的】 研究嗜酸光合细菌耦合萎缩芽孢杆菌在实验室条件下降解马铃薯淀粉废水污染物。【方法】 以马铃薯加工淀粉废水为材料,实验室条件下研究2种菌株复配比例、复合菌接种量、复合菌协同培养方式等对淀粉废水COD和恶臭气体的降解效率。【结果】 嗜酸光合细菌∶萎缩芽孢杆菌活菌数比=10∶1,接种量15%,30℃,采用分段连续培养前72 h厌氧光照(5000 lux)培养,之后72 h采取厌氧光照∶黑暗好氧=12 h∶12 h方式。在最优条件下添加1 g/L乙酸钠能够使淀粉废水COD降解率由对照组的16.9%提升到98.21%,水体中氨氮,硝氮,总磷,硫醇,硫化氢等污染物降解效率均超过98%以上,同时较高的pH使水体实现自絮凝,沉降速度达到8 cm/h。【结论】 嗜酸性光合细菌和萎缩芽孢杆菌最适生长pH差异造成的生态位分化是其耦合以及高效降解淀粉废水污染物的主要原因。

关键词: 嗜酸光合细菌, 萎缩芽孢杆菌, 马铃薯淀粉废水, 耦合机制

CLC Number:

Q935
S182

ZENG Jun, WU Lei, GAO Yan, YANG Hongmei, LIN Qing, HUO Xiangdong. Optimization of experimental conditions for the treatment of potato starch wastewater by coupling of acidophilic photosynthetic bacteria and Bacillus atrophaeus[J]. Xinjiang Agricultural Sciences, 2023, 60(11): 2798-2805.

曾军, 武磊, 高雁, 杨红梅, 林青, 霍向东. 嗜酸光合细菌耦合萎缩芽孢杆菌降解马铃薯加工淀粉废水条件优化[J]. 新疆农业科学, 2023, 60(11): 2798-2805.

Figures/Tables 7

Fig.1 Effect of tomato starch wastewater on the growth of Rhodoblastus sphagnicola and Bacillus atrophaeus Note:A: The growth curve of Rhodoblastus sphagnicola; B: The growth curve of Bacillus atrophaeus

Fig.2 Effect of tomato starch wastewater on the growth of the compound strains Note:A: Effect of adding Rhodoblastus sphagnicola on the growth of Bacillus atrophaeus; B: Effect of adding Bacillus atrophaeus on the growth of Rhodoblastus sphagnicola; C: The dynamic pH curves of the compound strains during the treatment of tomato starch wastewater

Fig.3 Effect of component ratio of the Rhodoblastus sphagnicola and Bacillus atrophaeus on the degradation efficiency of the tomato starch wastewater Note:A: Comparisons of degradation efficiency of the tomato starch wastewater by strains of different component ratios; B: Comparisons of pH values after degradation by strains of different component ratios; C: Comparisons of flocculation settlement after degradation by strains of different component ratios; D: Verification of the degradation rate by component ratio 10∶1 of the strains.

Fig.4 Effect of different inoculation rate of the compound strains on the degradation rate of potato starch wastewater

Fig.5 Effect of different culture conditions on the degradation rate of potato starch wastewater

Fig.6 Effect of different Sodium Acetate addition on the degradation rate of potato starch wastewater

Fig.7 Dynamic curves of tomato starch wastewater contaminant degradation by compound strains

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Optimization of experimental conditions for the treatment of potato starch wastewater by coupling of acidophilic photosynthetic bacteria and Bacillus atrophaeus

嗜酸光合细菌耦合萎缩芽孢杆菌降解马铃薯加工淀粉废水条件优化

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