Isolation and Identification of Pathogen of Potato Scab and Its Growth Characteristics from Zepu Area in Xinjiang

doi:10.6048/j.issn.1001-4330.2022.08.021

Xinjiang Agricultural Sciences ›› 2022, Vol. 59 ›› Issue (8): 1999-2006.DOI: 10.6048/j.issn.1001-4330.2022.08.021

• Plant Protection·Agricultural Product Analysis and Detection·Agricultural Equipment Engineering and Mechanization • Previous Articles Next Articles

Isolation and Identification of Pathogen of Potato Scab and Its Growth Characteristics from Zepu Area in Xinjiang

SONG Suqin¹(), GAO Haifeng², LV Zhuo¹^,³, TANG Qiyong¹, GU Meiying¹, ZHANG Zhidong¹, CHU Min¹, ZHU Jing¹, WANG Wei¹

1. Xinjiang Key Laboratory of Special Environmental Microorganisms/ Research Institute of Applied Microbiology, Xinjiang Academy of Agricultural Sciences, Urumqi, 830091, China
2. Key Laboratory of Intergraded Management of Harmful Crop Vermin of China Northwestern Oasis, MOARA, Institute of Plant Protection,Xinjiang Academy of Agricultural Sciences,Urumqi 830091,China
3. College of Food and Pharmaceutical Sciences, Xinjiang Agricultural University, Urumqi, 830052, China

Received:2021-09-28 Online:2022-08-20 Published:2022-10-01
Correspondence author: SONG Suqin
Supported by:
Open Fund for Key Laboratory of Integrated Pest Management on Crop in Northwestern Oasis, Ministry of Agriculture and Rural Affairs, China(KFJJ202006);Basic Scientific R &D Program of Public Welfare Research Institutions of Xinjiang Uygur Autonomous Region of China(KY2018012);Basic Scientific R &D Program of Public Welfare Research Institutions of Xinjiang Uygur Autonomous Region of China(KY2019020)

马铃薯疮痂病病原菌的分离鉴定及其生长特性分析

宋素琴¹(), 高海峰², 吕卓¹^,³, 唐琦勇¹, 顾美英¹, 张志东¹, 楚敏¹, 朱静¹, 王玮¹

1.新疆农业科学院微生物应用研究所/新疆特殊环境微生物重点实验室,乌鲁木齐 830091
2.新疆农业科学院植物保护研究所/农业部西北荒漠绿洲作物有害生物综合治理重点实验室,乌鲁木齐 830091
3.新疆农业大学食品科学与药学学院,乌鲁木齐 830052

通讯作者: 宋素琴
作者简介:宋素琴（1976-）,女,副研究员,研究方向为微生物活性产物及作物病害,（E-mail） suqin song@163.com
基金资助:
农业部西北荒漠绿洲作物有害生物综合治理重点实验室开放基金资助课题(KFJJ202006);自治区公益性科研院所基本科研业务经费(KY2018012);自治区公益性科研院所基本科研业务经费(KY2019020)

Abstract

Abstract:

【Objective】 The purpose of this study is to determine the species and growth characteristics of potato scab pathogen in Aikule Township, Kashgar Prefecture, Xinjiang, for the potato scab disease affected the yield and quality of potato seriously, and caused great losses to potato industry, especially to virus-free seed potato in Xinjiang. 【Method】 The pathogen was isolated and identified by tissue separation method combined with Koch's rule, microscopic morphology and 16 S rDNA. Then different carbon sources and nitrogen sources were selected to test its growth characteristics. 【Result】 Based on its morphological characteristics and 16S rDNA sequence, the pathogen of this disease was identified as Streptomyces acidiscabies from this area, which produced white-smooth curved spores with melanin and soluble pigment. Eight kinds of the carbon sources and two kinds of nitrogen sources were tested its growth period, such as glucose, methionine（Met） and histidine（His） etc. It could produce reticulate netlike scab after inoculatedon healthy potato tubers by smearing the tuber skin with spores suspension. The optimum growth temperature was 30℃, the pH was 7, and the optimum carbon and nitrogen sources were glucose and methionine separately. 【Conclusion】 The pathogen of the potato scab are isolated and identified from this area, and its growth characteristics were proved in this study, which would be the beneficial research basis for controlling this disease in Xinjiang.

Key words: potato common scab; isolation and identification; germicide; antagonists

摘要：

【目的】研究新疆喀什泽普县阿依库勒乡马铃薯疮痂病病原菌的种类及其生长特性。【方法】采用组织分离法结合柯赫氏法则、显微形态和16S rDNA等方法,分离鉴定马铃薯疮痂病病原菌;分析菌株对不同碳源、氮源的利用能力,以及不同pH的生长能力。【结果】新疆泽普马铃薯疮痂病的病原菌为疮痂链霉菌（Streptomyces acidiscabies）,产白色光滑呈自由弯曲状的孢子,能产生黑色素和可溶性色素,可利用葡萄糖等8种碳源和甲硫氨酸（Met）和组氨酸（His）2种氮源,接种健康马铃薯块茎上能产生网纹状甚至凹陷裂口状病斑。【结论】该地区马铃薯疮痂病病原菌最适生长温度为30℃,pH为7,最适碳氮源为葡萄糖和甲硫氨酸,全黑暗利于菌株的生长。

关键词: 马铃薯疮痂病, 分离鉴定, 杀菌剂, 拮抗菌

CLC Number:

S436.22

SONG Suqin, GAO Haifeng, LV Zhuo, TANG Qiyong, GU Meiying, ZHANG Zhidong, CHU Min, ZHU Jing, WANG Wei. Isolation and Identification of Pathogen of Potato Scab and Its Growth Characteristics from Zepu Area in Xinjiang[J]. Xinjiang Agricultural Sciences, 2022, 59(8): 1999-2006.

宋素琴, 高海峰, 吕卓, 唐琦勇, 顾美英, 张志东, 楚敏, 朱静, 王玮. 马铃薯疮痂病病原菌的分离鉴定及其生长特性分析[J]. 新疆农业科学, 2022, 59(8): 1999-2006.

Figures/Tables 9

Table 1 16s rDNA sequence Amplification of conventional PCR reaction system

Form	Volume（µL）
2XMix	25
F(10 μM)	1
R(10 μM)	1
Bacterial templates	1
ddH₂O	23

Fig. 1 Morphological characteristics of ZPCJ-1 Note： A：positive colony map of strain; B：reverse colony map of strain; C：micromorphology of strain（100×）;D：micromorphology of strain（40×）

Fig. 2 Determination of pathogenicity of pathogenic bacteria ZPCJ-1 Note：A：Surface map of potato inoculated with bacteria;B：the punch method;C、F：the pathogenicity of the pathogen to the potato skin;D：the acupuncture method;E：experiment on isolated leaves（120 d）

Table 2 Biological characteristics of ZPCJ-1

Cultural characteristic	Result	Cultural characteristic	Result
Spore morphology	Smooth, free to bend	Inose	++
Spore color	white	Utilization of nitrogen sources
Melanin	+++	Met	+++
Soluble pigment	+++	His	+
Utilization of carbon sources		Sensitivity experiment
Amylaceum	+++	Streptomycin	+++
Saccharose	+++	Penicillin	+++
Fructopyranose	++	Phenol	+++
Wood sugar	++	Crystal violet	+++
Mannitol	++	Starch utilization	+++
Arabinose	++	pH	4.0
Rhamnose	++	/	/

Fig. 3 Effect of temperature on ZPCJ-1 growth of strain Note：Differentlowercaselettersindicatesignificant differenceat P <0.05level,the same as below.

Fig. 4 Effect of light condition on ZPCJ-1 growth of strain

Fig. 5 Effect of pH on ZPCJ-1 growth of strain

Fig. 6 Effect of carbon and nitrogen sources on ZPCJ-1 growth of strain

Fig. 7 PCR amplification electrophoresis map and construction of phylogenetic tree for identification of ZPCJ-1

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Isolation and Identification of Pathogen of Potato Scab and Its Growth Characteristics from Zepu Area in Xinjiang

马铃薯疮痂病病原菌的分离鉴定及其生长特性分析

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