Xinjiang Agricultural Sciences ›› 2022, Vol. 59 ›› Issue (8): 1999-2006.DOI: 10.6048/j.issn.1001-4330.2022.08.021
• Plant Protection·Agricultural Product Analysis and Detection·Agricultural Equipment Engineering and Mechanization • Previous Articles Next Articles
SONG Suqin1(), GAO Haifeng2, LV Zhuo1,3, TANG Qiyong1, GU Meiying1, ZHANG Zhidong1, CHU Min1, ZHU Jing1, WANG Wei1
Received:
2021-09-28
Online:
2022-08-20
Published:
2022-10-01
Correspondence author:
SONG Suqin
Supported by:
宋素琴1(), 高海峰2, 吕卓1,3, 唐琦勇1, 顾美英1, 张志东1, 楚敏1, 朱静1, 王玮1
通讯作者:
宋素琴
作者简介:
宋素琴(1976-),女,副研究员,研究方向为微生物活性产物及作物病害,(E-mail) suqin song@163.com
基金资助:
CLC Number:
SONG Suqin, GAO Haifeng, LV Zhuo, TANG Qiyong, GU Meiying, ZHANG Zhidong, CHU Min, ZHU Jing, WANG Wei. Isolation and Identification of Pathogen of Potato Scab and Its Growth Characteristics from Zepu Area in Xinjiang[J]. Xinjiang Agricultural Sciences, 2022, 59(8): 1999-2006.
宋素琴, 高海峰, 吕卓, 唐琦勇, 顾美英, 张志东, 楚敏, 朱静, 王玮. 马铃薯疮痂病病原菌的分离鉴定及其生长特性分析[J]. 新疆农业科学, 2022, 59(8): 1999-2006.
Form | Volume(µL) |
---|---|
2XMix | 25 |
F(10 μM) | 1 |
R(10 μM) | 1 |
Bacterial templates | 1 |
ddH2O | 23 |
Table 1 16s rDNA sequence Amplification of conventional PCR reaction system
Form | Volume(µL) |
---|---|
2XMix | 25 |
F(10 μM) | 1 |
R(10 μM) | 1 |
Bacterial templates | 1 |
ddH2O | 23 |
Fig. 1 Morphological characteristics of ZPCJ-1 Note: A:positive colony map of strain; B:reverse colony map of strain; C:micromorphology of strain(100×);D:micromorphology of strain(40×)
Fig. 2 Determination of pathogenicity of pathogenic bacteria ZPCJ-1 Note:A:Surface map of potato inoculated with bacteria;B:the punch method;C、F:the pathogenicity of the pathogen to the potato skin;D:the acupuncture method;E:experiment on isolated leaves(120 d)
Cultural characteristic | Result | Cultural characteristic | Result |
---|---|---|---|
Spore morphology | Smooth, free to bend | Inose | ++ |
Spore color | white | Utilization of nitrogen sources | |
Melanin | +++ | Met | +++ |
Soluble pigment | +++ | His | + |
Utilization of carbon sources | Sensitivity experiment | ||
Amylaceum | +++ | Streptomycin | +++ |
Saccharose | +++ | Penicillin | +++ |
Fructopyranose | ++ | Phenol | +++ |
Wood sugar | ++ | Crystal violet | +++ |
Mannitol | ++ | Starch utilization | +++ |
Arabinose | ++ | pH | 4.0 |
Rhamnose | ++ | / | / |
Table 2 Biological characteristics of ZPCJ-1
Cultural characteristic | Result | Cultural characteristic | Result |
---|---|---|---|
Spore morphology | Smooth, free to bend | Inose | ++ |
Spore color | white | Utilization of nitrogen sources | |
Melanin | +++ | Met | +++ |
Soluble pigment | +++ | His | + |
Utilization of carbon sources | Sensitivity experiment | ||
Amylaceum | +++ | Streptomycin | +++ |
Saccharose | +++ | Penicillin | +++ |
Fructopyranose | ++ | Phenol | +++ |
Wood sugar | ++ | Crystal violet | +++ |
Mannitol | ++ | Starch utilization | +++ |
Arabinose | ++ | pH | 4.0 |
Rhamnose | ++ | / | / |
Fig. 3 Effect of temperature on ZPCJ-1 growth of strain Note:Differentlowercaselettersindicatesignificant differenceat P <0.05level,the same as below.
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