Isolation and Identification of Pathogen of Potato Scab and Its Growth Characteristics from Zepu Area in Xinjiang

doi:10.6048/j.issn.1001-4330.2022.08.021

Xinjiang Agricultural Sciences ›› 2022, Vol. 59 ›› Issue (8): 1999-2006.DOI: 10.6048/j.issn.1001-4330.2022.08.021

• Plant Protection·Agricultural Product Analysis and Detection·Agricultural Equipment Engineering and Mechanization • Previous Articles Next Articles

Isolation and Identification of Pathogen of Potato Scab and Its Growth Characteristics from Zepu Area in Xinjiang

SONG Suqin¹(), GAO Haifeng², LV Zhuo¹^,³, TANG Qiyong¹, GU Meiying¹, ZHANG Zhidong¹, CHU Min¹, ZHU Jing¹, WANG Wei¹

1. Xinjiang Key Laboratory of Special Environmental Microorganisms/ Research Institute of Applied Microbiology, Xinjiang Academy of Agricultural Sciences, Urumqi, 830091, China
2. Key Laboratory of Intergraded Management of Harmful Crop Vermin of China Northwestern Oasis, MOARA, Institute of Plant Protection,Xinjiang Academy of Agricultural Sciences,Urumqi 830091,China
3. College of Food and Pharmaceutical Sciences, Xinjiang Agricultural University, Urumqi, 830052, China

Received:2021-09-28 Online:2022-08-20 Published:2022-10-01
Correspondence author: SONG Suqin
Supported by:
Open Fund for Key Laboratory of Integrated Pest Management on Crop in Northwestern Oasis, Ministry of Agriculture and Rural Affairs, China(KFJJ202006);Basic Scientific R &D Program of Public Welfare Research Institutions of Xinjiang Uygur Autonomous Region of China(KY2018012);Basic Scientific R &D Program of Public Welfare Research Institutions of Xinjiang Uygur Autonomous Region of China(KY2019020)

马铃薯疮痂病病原菌的分离鉴定及其生长特性分析

宋素琴¹(), 高海峰², 吕卓¹^,³, 唐琦勇¹, 顾美英¹, 张志东¹, 楚敏¹, 朱静¹, 王玮¹

1.新疆农业科学院微生物应用研究所/新疆特殊环境微生物重点实验室,乌鲁木齐 830091
2.新疆农业科学院植物保护研究所/农业部西北荒漠绿洲作物有害生物综合治理重点实验室,乌鲁木齐 830091
3.新疆农业大学食品科学与药学学院,乌鲁木齐 830052

通讯作者: 宋素琴
作者简介:宋素琴（1976-）,女,副研究员,研究方向为微生物活性产物及作物病害,（E-mail） suqin song@163.com
基金资助:
农业部西北荒漠绿洲作物有害生物综合治理重点实验室开放基金资助课题(KFJJ202006);自治区公益性科研院所基本科研业务经费(KY2018012);自治区公益性科研院所基本科研业务经费(KY2019020)

Abstract

Abstract:

【Objective】 The purpose of this study is to determine the species and growth characteristics of potato scab pathogen in Aikule Township, Kashgar Prefecture, Xinjiang, for the potato scab disease affected the yield and quality of potato seriously, and caused great losses to potato industry, especially to virus-free seed potato in Xinjiang. 【Method】 The pathogen was isolated and identified by tissue separation method combined with Koch's rule, microscopic morphology and 16 S rDNA. Then different carbon sources and nitrogen sources were selected to test its growth characteristics. 【Result】 Based on its morphological characteristics and 16S rDNA sequence, the pathogen of this disease was identified as Streptomyces acidiscabies from this area, which produced white-smooth curved spores with melanin and soluble pigment. Eight kinds of the carbon sources and two kinds of nitrogen sources were tested its growth period, such as glucose, methionine（Met） and histidine（His） etc. It could produce reticulate netlike scab after inoculatedon healthy potato tubers by smearing the tuber skin with spores suspension. The optimum growth temperature was 30℃, the pH was 7, and the optimum carbon and nitrogen sources were glucose and methionine separately. 【Conclusion】 The pathogen of the potato scab are isolated and identified from this area, and its growth characteristics were proved in this study, which would be the beneficial research basis for controlling this disease in Xinjiang.

Key words: potato common scab; isolation and identification; germicide; antagonists

摘要：

【目的】研究新疆喀什泽普县阿依库勒乡马铃薯疮痂病病原菌的种类及其生长特性。【方法】采用组织分离法结合柯赫氏法则、显微形态和16S rDNA等方法,分离鉴定马铃薯疮痂病病原菌;分析菌株对不同碳源、氮源的利用能力,以及不同pH的生长能力。【结果】新疆泽普马铃薯疮痂病的病原菌为疮痂链霉菌（Streptomyces acidiscabies）,产白色光滑呈自由弯曲状的孢子,能产生黑色素和可溶性色素,可利用葡萄糖等8种碳源和甲硫氨酸（Met）和组氨酸（His）2种氮源,接种健康马铃薯块茎上能产生网纹状甚至凹陷裂口状病斑。【结论】该地区马铃薯疮痂病病原菌最适生长温度为30℃,pH为7,最适碳氮源为葡萄糖和甲硫氨酸,全黑暗利于菌株的生长。

关键词: 马铃薯疮痂病, 分离鉴定, 杀菌剂, 拮抗菌

CLC Number:

S436.22

SONG Suqin, GAO Haifeng, LV Zhuo, TANG Qiyong, GU Meiying, ZHANG Zhidong, CHU Min, ZHU Jing, WANG Wei. Isolation and Identification of Pathogen of Potato Scab and Its Growth Characteristics from Zepu Area in Xinjiang[J]. Xinjiang Agricultural Sciences, 2022, 59(8): 1999-2006.

宋素琴, 高海峰, 吕卓, 唐琦勇, 顾美英, 张志东, 楚敏, 朱静, 王玮. 马铃薯疮痂病病原菌的分离鉴定及其生长特性分析[J]. 新疆农业科学, 2022, 59(8): 1999-2006.

Figures/Tables 9

Table 1 16s rDNA sequence Amplification of conventional PCR reaction system

Form	Volume（µL）
2XMix	25
F(10 μM)	1
R(10 μM)	1
Bacterial templates	1
ddH₂O	23

Fig. 1 Morphological characteristics of ZPCJ-1 Note： A：positive colony map of strain; B：reverse colony map of strain; C：micromorphology of strain（100×）;D：micromorphology of strain（40×）

Fig. 2 Determination of pathogenicity of pathogenic bacteria ZPCJ-1 Note：A：Surface map of potato inoculated with bacteria;B：the punch method;C、F：the pathogenicity of the pathogen to the potato skin;D：the acupuncture method;E：experiment on isolated leaves（120 d）

Table 2 Biological characteristics of ZPCJ-1

Cultural characteristic	Result	Cultural characteristic	Result
Spore morphology	Smooth, free to bend	Inose	++
Spore color	white	Utilization of nitrogen sources
Melanin	+++	Met	+++
Soluble pigment	+++	His	+
Utilization of carbon sources		Sensitivity experiment
Amylaceum	+++	Streptomycin	+++
Saccharose	+++	Penicillin	+++
Fructopyranose	++	Phenol	+++
Wood sugar	++	Crystal violet	+++
Mannitol	++	Starch utilization	+++
Arabinose	++	pH	4.0
Rhamnose	++	/	/

Fig. 3 Effect of temperature on ZPCJ-1 growth of strain Note：Differentlowercaselettersindicatesignificant differenceat P <0.05level,the same as below.

Fig. 4 Effect of light condition on ZPCJ-1 growth of strain

Fig. 5 Effect of pH on ZPCJ-1 growth of strain

Fig. 6 Effect of carbon and nitrogen sources on ZPCJ-1 growth of strain

Fig. 7 PCR amplification electrophoresis map and construction of phylogenetic tree for identification of ZPCJ-1

References 26

[1]	Loria R., Coombs J., Yoshida M., et al. A paucity of bacterial root diseases: Streptomyces succeeds where others fail[J]. Physiological and Molecular Plant Pathology, 2003, 62:65-72. DOI URL
[2]	杜娟, 任娟, 赵思峰, 等. 新疆马铃薯疮痂病病原的鉴定[J]. 石河子大学学报(自然科学版), 2010, 28(4):414-417.
	DU Juan, REN Juan, ZHAO Sifeng, et al. Identification of the pathogen of potato common scab in Xinjiang[J]. Journal of Shihezi University, 2010, 28(4):414-417.
[3]	李驰, 刘艳, 梁燕, 等. 马铃薯疮痂病病原菌鉴定及其生物学特性[J]. 农业生物技术学报, 2019, 27(5):897-907.
	LI Chi, LIU Yan, LIANG Yan, et al. Identification and biological characteristics of potato common scab pathogen[J]. Journal of Agricultural Biotechnology, 2019, 27(5):897-907.
[4]	张萌. 中国不同地区马铃薯疮痂病菌遗传多样性分析[D]. 保定: 河北农业大学, 2008.
	ZHANG Meng. Genetic diversity among Stretomyces isolates causing potato common scabin different regions of China[D]. Baoding: Hebei Agricultural University, 2008.
[5]	方中达. 植病研究方法[M]. 3版. 北京: 中国农业出版社, 2001.
	FANG Zhongda. Methods of study on phytopathy[M]. Version 3. Beijing: China Agricultural Publishing House, 2001.
[6]	王丽丽, 李洪涛, 日孜旺古丽•苏皮色来, 等. 乌昌地区窖藏马铃薯菌物病害调查及病原鉴定[J]. 新疆农业大学学报, 2014, 37(6):469-473.
	WANG Lili, LI Hongtao, RI Ziwangli supiselai, et al. Investigation and identification of fungus disease and pathogens on storage period in Urumqi and Changji areas of Xinjiang[J]. Journal of Xinjiang Agricultural University, 2014, 37(6):469-473.
[7]	李玉聪, 李滨影, 油心怡, 等. 马铃薯疮痂病拮抗菌的筛选鉴定及防治效果初探[J]. 生物技术通报, 2018, 34(10):116-121. DOI
	LI Yucong, LI Binying, YOU Xinyi, et al. Screening and identification of antagonistic bacteria of potato common scab disease and its control effect[J]. Biotechnology Bulletin, 2018, 34(10):116-121. DOI
[8]	张良, 程林润, 卞晓波, 等. 中国马铃薯疮痂病的研究与防控[J]. 浙江农业科学, 2019, 60(10):1778-1781.
	ZHANG Liang, CHENG Linrun, BIAN Xiaobo, et al. Study and control of potato common scab disease in China[J]. Zhejiang Agricultural Science, 2019, 60(10):1778-1781.
[9]	Dees M W, Wanner L A. In Search of Better Management of Potato Common Scab[J]. PotatoRes, 2012, 55(3):249-268.
[10]	赵伟全, 杨文香, 李亚宁, 等. 中国马铃薯疮痂病菌的鉴定[J]. 中国农业科学, 2006,(2):313-318.
	ZHAO Weiquan, YANG Wenxiang, LI Yaning, er al. Characterization and Identification on the Pathogen of Potato Scab in China[J]. Scientia Agricultura Sinica, 2006, 39(2): 313-318.
[11]	邢莹莹, 吕典秋, 魏琪, 等. 黑龙江省部分地区马铃薯疮痂病菌种类及致病性鉴定[J]. 植物保护, 2016, 42(1):26-32,50.
	XING Yingying, LV Dianqiu, WEI Qi, et al. Species and pathogenicity identification of Streptomyces species causing potato common scab in part of Heilongjiang Province[J]. Plant Protection, 2016, 42(1): 26-32,50.
[12]	王丹, 姚晓东, 李新凤, 等. 山西晋城马铃薯疮痂病病原菌鉴定[J]. 山西农业大学学报(自然科学版), 2015, 35(5):495-498.
	WANG Dan, YAO Xiaodong, LI Xinfeng, et al. Identification of The Pathogen of Potato Scab in Jincheng of Shanxi Province[J]. Journal of Shanxi Agricultural University, 2015, 35(5): 495-498.
[13]	康蓉. 甘肃马铃薯疮痂病病原鉴定及药剂防治[D]. 兰州: 甘肃农业大学, 2013.
	KANG Rong. Identification of pathogen and control of potato scab in Gansu[D]. Lanzhou, Gansu Agricultural University, 2013.
[14]	张铭铄. 一株马铃薯疮痂病病原菌的系统鉴定及生物防治的研究[D]. 泰安: 山东农业大学, 2016.
	ZHANG Mingshuo. Systematic identificationof a potato common scab pathogen and its biological control[D]. Taian: Shandong Agricultural University, 2016.
[15]	Investigation of Streptomyces scabies causing potato scab by various detection techniques, its pathogenicity and determination of Host-Disease Resistance in potato germplasm.[J]. Pathogens Basel, Switzerland, 2020, 9(9).
[16]	Mandeep Singh Hunjan, Hardeep Singh Sabhikhi. Designing a crop rotation strategy to manage Streptomyces scabies causing potato scab in north India[J]. Journal of Phytopathology, 2020, 168(7-8).
[17]	陈利达, 谢学文, 石延霞, 等. 酸疮痂链霉菌实时荧光定量PCR检测体系的构建及应用[J]. 农业生物技术学报, 2020, 28(7):1314-1321.
	CHENG Lida, XIE Xuewen, SHI Yanxia, et al. Establishment and Application of Real-time Quantitative PCR for Detecting of Streptomyces acidiscabies[J]. Journal of Agricultural Biotechnology, 2020, 28(7):1314-1321.
[18]	陈志垚, 台莲梅, 王天君, 等. 马铃薯疮痂病链霉菌的生物学特性研究[J]. 黑龙江农业科学, 2020,(7):71-74.
	CHEN Zhiyao, TAI Llianmei, WANG Tianjun, et al. Study on Biological Characteristics of Streptomyces scabies[J]. Heilongjiang Agricultural Sciences, 2020,(7):71-74.
[19]	JIA Zhengyuan, Benoît Bizimungu, David De Koeyer, et al. Genome-Wide Association Study of Resistance to Potato Common Scab[J]. Potato Research: Journal of the European Association for Potato Research, 2020, 63(2).
[20]	马建荣, 余永红, 黎敬鸿, 等. 马铃薯疮痂病链霉新变种的分离与生物学特性分析[J]. 安徽农业科学, 2019, 47(18):139-142.
	MA Jianrong, YU Yonghong, LI Jinghong, et al. Isolation and Biological Characteristics Analysis of a New Variety of Streptomyces Potato Scab[J]. J.Anhui Agric.Sci, 2019, 47(18) : 139-142.
[21]	聂峰杰, 巩檑, 张丽, 等. 马铃薯疮痂病抗病育种展望[J]. 宁夏农林科技, 2020, 61(5):56-57.
	NIE Fengjie, GONG Lei, ZHANG Li, et al. Prospect of Disease Resistance Breeding for Potato Common Scab[J]. Ningxia Journal of Agri. and Fores. Sci.&Tech. 2020, 61(5):56-57.
[22]	崔凌霄, 杨成德, 魏立娟, 等. 甘肃省定西市马铃薯疮痂病新病原Streptomyces galilaeus的分离、鉴定及生物学特性研究[J]. 植物保护, 2018, 44(5):206-211.
	CUI Lingxiao, YANG Chengde, WEI Lijuan, et al. Study on Isolation, Identification and Biological Characteristics of New Pathogenic Streptomyces galilaeus of Potato Scab in Dingxi City, Gansu Province[J]. Plant Protection, 2018, 44(5):206-211.
[23]	熊悯梓, 沙亚鹏, 赵盼, 等. 不同生境马铃薯根际土壤细菌多样性分析[J]. 微生物学报, 2020, 60(11):2434-2449.
	XONG Minzi, SHA Yapeng, ZHAO Pan, et al. Comparison of bacterial diversity in rhizosphere soil of potato in different habitats[J]. Acta Microbiologica Sinica 2020, 60(11): 2434-2449.
[24]	Wencong Shi, Mingcong Li, Guangshan Wei, et al. The occurrence of potato common scab correlates with the community composition and function of the geocaulosphere soil microbiome[J]. 2019, 7(1).
[25]	LI Yuting, LIU Jingyu, Díaz-Cruz Gustavo, et al. Virulence mechanisms of plant-pathogenic Streptomyces species: an updated review.[J]. Microbiology, 2019, 165(10).
[26]	LIANG Feiyang, LIN Rongshan, YAO Yaqian, et al. Systematic Identification of Pathogenic Streptomyces sp. AMCC400023 That Causes Common Scab and Genomic Analysis of Its Pathogenicity Island.[J]. Phytopathology, 2019, 109(7).

Isolation and Identification of Pathogen of Potato Scab and Its Growth Characteristics from Zepu Area in Xinjiang

马铃薯疮痂病病原菌的分离鉴定及其生长特性分析

RichHTML

PDF

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 9

References 26

Related Articles 10

Recommended Articles

Metrics

[1]	SHEN Hongfei, JIA Fenglian, LIU Yi, WU Yan, YANG Ruwei, SUN Hui, LI Guangyue. Isolation and identification of the pathogen for potato black scurf in Xinjiang [J]. Xinjiang Agricultural Sciences, 2024, 61(7): 1766-1771.
[2]	YU Lyujian, YANG Jin, DING Yu, LI Xiaoman, LIU Fengjuan, FAN Yingying, LIANG Hongyu, JIAO Ziwei, WANG Cheng. Isolation,identification and pathogenicity of fungi on the surface of pomegranate fruit [J]. Xinjiang Agricultural Sciences, 2024, 61(1): 148-155.
[3]	XU Lijuan, CHEN Yong, WANG Zeyu, WANG Bo, Ainijiang Ersiman, GUO Rui, LI Kemei, SONG Suqin. Isolation, identification and biological characteristics of potato scab pathogen in Baicheng, Xinjiang [J]. Xinjiang Agricultural Sciences, 2023, 60(9): 2258-2265.
[4]	GAO Yonggang, GAO Haifeng, LÜ Zhuo, XU lijuan, , TANG Qiyong, GU Meiying, ZHANG Lijuan, ZHU Jinquan, SONG Suqin. Investigation into the Diseases of the Potato in Different Producing Areas in Xinjiang [J]. Xinjiang Agricultural Sciences, 2022, 59(1): 170-178.
[5]	LÜ Zhuo, GAO Yonggang, TANG Qiyong, CHU Min, GU Meiying, ZHU Jing, CHENG Siqi, MA Xiaoxue, SONG Suqin, WANG Jing. Preliminary Identification of the Pathogen of Potato Disease in Qitai [J]. Xinjiang Agricultural Sciences, 2020, 57(5): 902-909.
[6]	HAN Meng-li, ZHANG Xing-xing, WU Tong-zhong, GUO Qiang-qiang, HUANG Xin, ZHONG Fa-gang. Isolation and Identification of Pasteurella multocida Rabbits [J]. Xinjiang Agricultural Sciences, 2018, 55(7): 1333-1342.
[7]	DAI Jing, PENG Bin, LEI Chen-hong, Aray Hayrat. Isolation,Identification and Drug Resistance Detection of Campylobacter Jejuni from Cattle in Partial Areas of Xinjiang [J]. Xinjiang Agricultural Sciences, 2017, 54(9): 1730-1736.
[8]	YANG Xue-yun;LI Jian-jun;WANG Shuo;WU Jian-yong;WANG Deng-feng;WANG Zhi-cai. Evaluation of the Modified Granada Medium for Detection of Streptococcus agalactiae from Mastitis Samples [J]. , 2014, 51(11): 2093-2098.
[9]	LIU Xiao-rong;XIANG Ting-jian;PANG Huan-ming;Gulinazi;LI Jin-yu;WANG Zi-rong. Isolation and Identification of Lactic Acid Bacteria from Tradional Yogurt in Urumqi and Changji Region [J]. , 2013, 50(8): 1434-1441.
[10]	ZHANG Xiang-feng;Ainiwaer·Aishan;Mairemunisha·Wufuer;YAN Jing-yang;Yusufu·Rexiti. Isolation and Identification of Lactobacillus from Xinjiang Small Reed Silage [J]. , 2009, 46(6): 1327-1331.