Responses of Soil Ammonia Oxidizers and Denitrifiers to Poly (Butylene Adipate-Co-Terephthalate) -Degrading Mulch

doi:10.6048/j.issn.1001-4330.2019.07.014

Xinjiang Agricultural Sciences ›› 2019, Vol. 56 ›› Issue (7): 1303-1311.DOI: 10.6048/j.issn.1001-4330.2019.07.014

Responses of Soil Ammonia Oxidizers and Denitrifiers to Poly (Butylene Adipate-Co-Terephthalate) -Degrading Mulch

LIN Qing¹, ZENG Jun¹, WANG Bing², SHI Ying-wu¹, YANG Hong-mei¹, ZHANG Tao¹, GAO Yan¹, CHU Ming¹, WANG Jin-xin², SUN Jiu-sheng², HUO Xiang-dong¹

1. Institute of Applied Microbiology, Xinjiang Academy of Agricultural Sciences, /Xinjiang Laboratory of Special Environmental Microbiology, Urumqi 830091, China:
2. Institute of Soil, Fertilizer and Agricultural Water Conservation, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China

Received:2018-04-12 Online:2019-07-20 Published:2019-09-05
Correspondence author: HUO Xiang-dong(1974-),associate professor,focus on microbial resources,(E-mail) xiangdonghuo@163.com
Supported by:
The Key Research and Development Program of Xinjiang Uygur Autonomous Region "Study on the impact of biodegradable mulch film on soil environment"(2016B02017-4); The National Natural Science Foundation of China "Metagenomic Library Construction of the Rumen Microbe from Xinjiang Bactrian Camel and Cellulase Family Research" (31160027);The Natural Science Foundation Project of Xinjiang Uygur Autonomous Region "Screening and Identification of Microbia with Growth-promoting, System Resistance Induction of Processing Tomato, and Their Efficiency"(2018D01B43)

聚对苯二甲酸-己二酸丁二酯生物降解膜对土壤氨氧化和反硝化微生物变化的影响

林青¹, 曾军¹, 王斌², 史应武¹, 杨红梅¹, 张涛¹, 高雁¹, 楚敏¹, 王金鑫², 孙九胜², 霍向东¹

1.新疆农业科学院微生物应用研究所/新疆特殊环境微生物实验室,乌鲁木齐 830091;
2.新疆农业科学院土壤肥料与农业节水研究所,乌鲁木齐 830091

通讯作者: 霍向东(1974-),男,甘肃人,副研究员,研究方向为微生物资源,(E-mail)xiangdonghuo@163.com
作者简介:林青(1985-),女,山东烟台人,助理研究员,硕士,研究方向为微生物生态,(E-mail)qinglinxj@163.com
基金资助:
新疆维吾尔自治区重点研发项目"生物降解地膜降解后对土壤环境的影响评价研究"(2016B02017-4); 国家自然科学基金"新疆双峰驼瘤胃微生物宏基因组文库构建和纤维素酶系的研究"(31160027);新疆维吾尔自治区自然科学基金项目"介导加工番茄诱导系统抗性促生菌的筛选鉴定及其抗病效能的研究"(2018D01B43)

Abstract

Abstract: 【Objective】 To study the effect of Poly (butylene adipate-co-terephthalate) biodegradation membrane on soil physicochemical properties and nitrogen-cycle-related microorganisms. 【Method】Compared with coated PE membrane, the number and community diversity of soil nitrogen cycle related microorganisms in coated PBAT biodegradable membrane were analyzed by real-time fluorescence quantitative PCR and terminal restriction fragment length polymorphism (T-RFLP). 【Result】There was no significant difference in soil physical and chemical properties of the same crops (except cotton) between PE membrane and PBAT biodegradable membrane. There was no significant difference of AOA-amoA, AOB-amoA and nosZ gene abundance between the same crops covered with PBAT biodegradable membrane and PE membrane, while the difference of AOA-amoA, AOB-amoA and nosZ gene abundance was significant for the different crops. 【Conclusion】There was no significant difference between the effects of PE membrane and PBAT biodegradable membrane on soil physical and chemical properties and nitrogen cycling microorganisms. PBAT biodegradable membrane can be used as a substitute for PE membrane to solve the white pollution caused by PE membrane.

Key words: poly (butylene adipate-co-terephthalate); mulch; ammonia oxidizers; denitrifiers

摘要： 【目的】研究聚对苯二甲酸-己二酸丁二酯(Poly(butylene adipate-co-terephthalate),PBAT)生物降解膜对土壤理化性质及氮循环相关微生物的影响。【方法】以铺覆PE膜为对照,利用实时荧光定量PCR和末端限制性片段长度多态性(T-RFLP)技术解析铺覆PBAT生物降解膜中土壤氮循环相关微生物的数量和群落多样性。【结果】同种作物(除棉花外)铺覆PE膜和PBAT生物降解膜土壤理化性质差异不显著。同种作物铺覆PBAT生物降解膜与PE膜之间,AOA-amoA、AOB-amoA及nosZ基因丰度无显著差异,而不同作物对AOA-amoA、AOB-amoA及nosZ基因丰度的影响较为明显。【结论】PE膜和PBAT生物降解膜对土壤理化性质及氮循环微生物的影响差异不显著, PBAT生物降解膜可以作为PE膜的替代品以解决PE膜造成的白色污染。

关键词: 聚对苯二甲酸-己二酸丁二酯, 生物降解, 氨氧化, 反硝化

CLC Number:

S188

LIN Qing, ZENG Jun, WANG Bing, SHI Ying-wu, YANG Hong-mei, ZHANG Tao, GAO Yan, CHU Ming, WANG Jin-xin, SUN Jiu-sheng, HUO Xiang-dong. Responses of Soil Ammonia Oxidizers and Denitrifiers to Poly (Butylene Adipate-Co-Terephthalate) -Degrading Mulch[J]. Xinjiang Agricultural Sciences, 2019, 56(7): 1303-1311.

林青, 曾军, 王斌, 史应武, 杨红梅, 张涛, 高雁, 楚敏, 王金鑫, 孙九胜, 霍向东. 聚对苯二甲酸-己二酸丁二酯生物降解膜对土壤氨氧化和反硝化微生物变化的影响[J]. 新疆农业科学, 2019, 56(7): 1303-1311.

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Responses of Soil Ammonia Oxidizers and Denitrifiers to Poly (Butylene Adipate-Co-Terephthalate) -Degrading Mulch

聚对苯二甲酸-己二酸丁二酯生物降解膜对土壤氨氧化和反硝化微生物变化的影响

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