Responses of Soil Ammonia Oxidizers and Denitrifiers to Poly (Butylene Adipate-Co-Terephthalate) -Degrading Mulch

doi:10.6048/j.issn.1001-4330.2019.07.014

Xinjiang Agricultural Sciences ›› 2019, Vol. 56 ›› Issue (7): 1303-1311.DOI: 10.6048/j.issn.1001-4330.2019.07.014

Responses of Soil Ammonia Oxidizers and Denitrifiers to Poly (Butylene Adipate-Co-Terephthalate) -Degrading Mulch

LIN Qing¹, ZENG Jun¹, WANG Bing², SHI Ying-wu¹, YANG Hong-mei¹, ZHANG Tao¹, GAO Yan¹, CHU Ming¹, WANG Jin-xin², SUN Jiu-sheng², HUO Xiang-dong¹

1. Institute of Applied Microbiology, Xinjiang Academy of Agricultural Sciences, /Xinjiang Laboratory of Special Environmental Microbiology, Urumqi 830091, China:
2. Institute of Soil, Fertilizer and Agricultural Water Conservation, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China

Received:2018-04-12 Online:2019-07-20 Published:2019-09-05
Correspondence author: HUO Xiang-dong(1974-),associate professor,focus on microbial resources,(E-mail) xiangdonghuo@163.com
Supported by:
The Key Research and Development Program of Xinjiang Uygur Autonomous Region "Study on the impact of biodegradable mulch film on soil environment"(2016B02017-4); The National Natural Science Foundation of China "Metagenomic Library Construction of the Rumen Microbe from Xinjiang Bactrian Camel and Cellulase Family Research" (31160027);The Natural Science Foundation Project of Xinjiang Uygur Autonomous Region "Screening and Identification of Microbia with Growth-promoting, System Resistance Induction of Processing Tomato, and Their Efficiency"(2018D01B43)

聚对苯二甲酸-己二酸丁二酯生物降解膜对土壤氨氧化和反硝化微生物变化的影响

林青¹, 曾军¹, 王斌², 史应武¹, 杨红梅¹, 张涛¹, 高雁¹, 楚敏¹, 王金鑫², 孙九胜², 霍向东¹

1.新疆农业科学院微生物应用研究所/新疆特殊环境微生物实验室,乌鲁木齐 830091;
2.新疆农业科学院土壤肥料与农业节水研究所,乌鲁木齐 830091

通讯作者: 霍向东(1974-),男,甘肃人,副研究员,研究方向为微生物资源,(E-mail)xiangdonghuo@163.com
作者简介:林青(1985-),女,山东烟台人,助理研究员,硕士,研究方向为微生物生态,(E-mail)qinglinxj@163.com
基金资助:
新疆维吾尔自治区重点研发项目"生物降解地膜降解后对土壤环境的影响评价研究"(2016B02017-4); 国家自然科学基金"新疆双峰驼瘤胃微生物宏基因组文库构建和纤维素酶系的研究"(31160027);新疆维吾尔自治区自然科学基金项目"介导加工番茄诱导系统抗性促生菌的筛选鉴定及其抗病效能的研究"(2018D01B43)

Abstract

Abstract: 【Objective】 To study the effect of Poly (butylene adipate-co-terephthalate) biodegradation membrane on soil physicochemical properties and nitrogen-cycle-related microorganisms. 【Method】Compared with coated PE membrane, the number and community diversity of soil nitrogen cycle related microorganisms in coated PBAT biodegradable membrane were analyzed by real-time fluorescence quantitative PCR and terminal restriction fragment length polymorphism (T-RFLP). 【Result】There was no significant difference in soil physical and chemical properties of the same crops (except cotton) between PE membrane and PBAT biodegradable membrane. There was no significant difference of AOA-amoA, AOB-amoA and nosZ gene abundance between the same crops covered with PBAT biodegradable membrane and PE membrane, while the difference of AOA-amoA, AOB-amoA and nosZ gene abundance was significant for the different crops. 【Conclusion】There was no significant difference between the effects of PE membrane and PBAT biodegradable membrane on soil physical and chemical properties and nitrogen cycling microorganisms. PBAT biodegradable membrane can be used as a substitute for PE membrane to solve the white pollution caused by PE membrane.

Key words: poly (butylene adipate-co-terephthalate); mulch; ammonia oxidizers; denitrifiers

摘要： 【目的】研究聚对苯二甲酸-己二酸丁二酯(Poly(butylene adipate-co-terephthalate),PBAT)生物降解膜对土壤理化性质及氮循环相关微生物的影响。【方法】以铺覆PE膜为对照,利用实时荧光定量PCR和末端限制性片段长度多态性(T-RFLP)技术解析铺覆PBAT生物降解膜中土壤氮循环相关微生物的数量和群落多样性。【结果】同种作物(除棉花外)铺覆PE膜和PBAT生物降解膜土壤理化性质差异不显著。同种作物铺覆PBAT生物降解膜与PE膜之间,AOA-amoA、AOB-amoA及nosZ基因丰度无显著差异,而不同作物对AOA-amoA、AOB-amoA及nosZ基因丰度的影响较为明显。【结论】PE膜和PBAT生物降解膜对土壤理化性质及氮循环微生物的影响差异不显著, PBAT生物降解膜可以作为PE膜的替代品以解决PE膜造成的白色污染。

关键词: 聚对苯二甲酸-己二酸丁二酯, 生物降解, 氨氧化, 反硝化

CLC Number:

S188

LIN Qing, ZENG Jun, WANG Bing, SHI Ying-wu, YANG Hong-mei, ZHANG Tao, GAO Yan, CHU Ming, WANG Jin-xin, SUN Jiu-sheng, HUO Xiang-dong. Responses of Soil Ammonia Oxidizers and Denitrifiers to Poly (Butylene Adipate-Co-Terephthalate) -Degrading Mulch[J]. Xinjiang Agricultural Sciences, 2019, 56(7): 1303-1311.

林青, 曾军, 王斌, 史应武, 杨红梅, 张涛, 高雁, 楚敏, 王金鑫, 孙九胜, 霍向东. 聚对苯二甲酸-己二酸丁二酯生物降解膜对土壤氨氧化和反硝化微生物变化的影响[J]. 新疆农业科学, 2019, 56(7): 1303-1311.

References 26

[1]	严昌荣, 何文清, 薛颖昊, 等. 生物降解地膜应用与地膜残留污染防控[J]. 生物工程学报, 2016, 32(6): 746-760.YAN Chang-rong, HE Wen-qin, XUE Ying-hao, et al. (2016). Application of biodegradable plastic film to reduce plastic film residual pollution in Chinese agriculture [J]. Chin J Biotech, 32(6): 748-760. (in Chinese)
[2]	何文清,刘琪,李元桥,等. 生物降解地膜新材料的发展及产业化前景[J]. 生物产业技术, 2017, (2):7-13.HE Wen-qing, LIU Qi, LI Yuan-qiao, et al. (2017). Development and industrial prospect of new biodegradable film materials [J]. Biotechnology & Business, (2): 7-13. (in Chinese)
[3]	李倩, 安洁,石宝翠, 等. 残膜污染防治与可降解地膜生态环境影响评价[J]. 山东农业科学, 2016, 48(12): 111-116.LI Qian, AN Jie, SHI Bao-cui, et al. (2016). Control of residual film pollution and ecological environment impact assessment of degradable mulch film [J]. Shandong Agricultural Sciences, 48(12): 111-116. (in Chinese)
[4]	吴凤全, 林涛, 祖米来提·吐尔干, 等. 降解地膜对南疆棉田土壤水热及棉花产量的影响[J]. 农业环境科学学报, 2018, 37(12): 2 793-2 801.WU Feng-quan, LIN Tao, Zumilaiti Tuergan, et al. (2018).Effects of degradable plastic mulching film on soil moisture, temperature, and yield in cotton fields in southern Xinjiang, China [J]. Journal of Agro-Environment Science, 37(12): 2,793-2,801. (in Chinese)
[5]	张占琴, 闫紫薇, 杨相昆, 等. 不同降解地膜降解性能及棉花应用效果研究[J].西南农业学报, 2018, 31(6): 1 185-1 189.ZHANG Zhan-qin, YAN Zi-wei, YANG Xiang-kun, et al. (2018). Degradation property of various degradable plastic film and application effect on cotton field [J]. Southwest China Journal of Agricultural Sciences, 31(6): 1,185-1,189. (in Chinese)
[6]	山立, 韩冰. 可降解农用地膜国内外研究推广进程与存在问题[J]. 陕西农业科学, 2015, (12):73-77.SHAN Li, HAN Bing. (2015). The generalization progress and the problem of degradable mulch at home and abroad [J].Shaanxi Journal of Agricultural Sciences, (12): 73-77. (in Chinese)
[7]	赵彩霞, 何文清, 刘爽, 等. 新疆地区全生物降解膜降解特征及其对棉花产量的影响[J]. 农业环境科学学报, 2011, 30(8): 1 616-1 621.ZHAO Cai-xia, HE Wen-qing, LIU Shuang, et al. (2011). Degradation of biodegradable plastic mulch film and its effect on the yield of cotton in Xinjiang region, China [J].Journal of Agro-Environment Science, 30(8):1,616-1,621. (in Chinese)
[8]	唐薇, 张冬梅, 徐士振, 等. 生物降解膜降解特征及其对棉花生长发育和产量的影响[J].中国棉花, 2016, 43(4): 21-24,28.TANG Wei, ZHANG Dong-mei, XU Shi-zhen, et al. (2016). The degradation of biodegradable plastic and its effect on the growth and yield of cotton [J]. China Cotton, 43(4): 21-24,28. (in Chinese)
[9]	朱友娟, 伍维模, 温善菊, 等. 可降解地膜对新疆南疆棉花生长和产量的影响[J].干旱地区农业研究, 2016, 34(4): 189-196,224.ZHU You-juan, WU Wei-mo, WEN Shan-ju, et al. (2016). The effect of degradable agricultural mulch films on the growth and yield of cotton in Southern Xinjiang [J]. Agricultural Research in the Arid Areas, 34(4): 189-196,224. (in Chinese)
[10]	胡宏亮, 韩之刚, 张国平. 生物降解地膜对玉米的生物学效应及其降解特性[J]. 浙江大学学报(农业与生命科学版), 2015, 41(2): 179-188.HU Hong-liang, HAN Zhi-gang, ZHANG Guo-ping. (2015). Biological effect of biodegradable mulch films on maize and their degradation properties [J]. Journal of Zhejiang University (Agric.＆Life Sci.), 41(2): 179-188. (in Chinese)
[11]	石凤兴. 生物降解膜在玉米上的应用效果研究[J]. 耕作与栽培, 2016, (2):37-38,29.SHI Feng-xing. (2016). Effect of the biodegradable film on maize [J]. Tillage and Cultivation, (2):37-38,29. (in Chinese)
[12]	林萌萌, 孙涛, 尹继乾, 等. 不同生物降解地膜对花生光合特性和产量的影响[J].中国农学通报, 2015,31(27):190-197.LIN Meng-meng, SUN Tao, YIN Ji-qian, et al. (2015). Effects of different biodegradable films mulching on the photosynthetic characteristics and yield of peanut [J]. Chinese Agricultural Science Bulletin, 31(27):190-197. (in Chinese)
[13]	Touchaleaume, F., Martin-Closas, L., Angellier-Coussy, H., et al. (2016). Performance and environmental impact of biodegradable polymers as agricultural mulching films. Chemosphere, 144: 433-439.
[14]	李桢, 王佳玮, 雷利国, 等. 地膜覆盖对辣椒地土壤氮素的影响[J].中国农学通报, 2016,32(21):134-140.LI Zhen, WANG Jia-wei, LEI Li-guo, et al. (2016). Effect of plastic film mulching on soil nitrogen in pepper field [J].Chinese Agricultural Science Bulletin, 32(21):134-140. (in Chinese)
[15]	倪雪, 郝庆菊, 陈世杰, 等. 地膜覆盖和施氮对菜地N2O排放的影响[J]. 环境科学, 2019, 40(2): 1-15.NI Xue, HAO Qing-ju, CHEN Shi-jie, et al. (2019). Effects of plastic film mulching and nitrogen fertilizer application on N2O emissions from a vegetable field [J]. Environmental Science, 40(2): 1-15. (in Chinese)
[16]	He, J. Z., Shen, J. P., Zhang, L. M. , Zhu, Y. G. , Zheng, Y. M. , & Xu, M. G., et al. (2007). Quantitative analyses of the abundance and composition of ammonia-oxidizing bacteria and ammonia-oxidizing archaea of a chinese upland red soil under long-term fertilization practices. Environmental microbiology,9(9), 2,364-2,374.
[17]	Blackwood, C. B., Marsh, T., Kim, S. H., & Paul, E. A. (2003). Terminal restriction fragment length polymorphism data analysis for quantitative comparison of microbial communities. Appl Environ Microbiol, 69(2):926-932.
[18]	李红. 末端限制性酶切片段长度多态性分析技术进展[J].安徽师范大学学报(自然科学版), 2006, 29(6): 582-585.LI Hong. (2006). Progress of T-RFLP analysis for microbial community [J].Journal of Anhui Normal University (Natural Science), 29(6): 582-585. (in Chinese)
[19]	Jin, T., Zhang, T., & Yan, Q. (2010). Characterization and quantification of ammonia-oxidizing archaea (aoa) and bacteria (aob) in a nitrogen-removing reactor using t-rflp and qpcr. Applied Microbiology and Biotechnology, 87(3): 1,167-1,176.
[20]	孙翼飞, 沈菊培, 张翠景, 等. 不同放牧强度下土壤氨氧化和反硝化微生物的变化特征[J]. 生态学报, 2018,38(8):2 874-2 883.SUN Yi-fei, SHEN Ju-pei, ZHANG Cui-jing, et al. (2018). Responses of soil ammonia oxidizers and denitrifiers to different grazing intensities[J].Acta Ecologica Sinica, 38(8):2,874-2,883. (In Chinese)
[21]	Hai, L., Li, X., Liu, X., Jiang, X., Guo, R., & Jing, G., et al. (2015). Plastic mulch stimulates nitrogen mineralization in urea-amended soils in a semiarid environment. AGRONOMY JOURNAL, 107(3):921-930.
[22]	宋文元. 地膜覆盖对土壤氮素矿化的影响[D]. 兰州大学, 2017.SONG Wen-yuan. (2017). Effects of plastic film mulching on soil nitrogen mineralization[D]. Master Dissertation. Lanzhou University. (In Chinese)
[23]	张景俊, 李仙岳, 彭遵原, 等. 河套灌区葵花农田生物地膜覆盖下土壤水-热-氮-盐分布特征[J].生态环境学报, 2018, 27(6): 1 067-1 075.ZHANG Jing-jun,LI Xian-yue, PENG Zun-yuan, et al. (2018). The distribution of soil water, temperature, nitrogen and salinity under biofilm mulching in Hetao sunflower field [J].Ecology and Environmental Sciences, 27(6): 1,067-1,075. (In Chinese)
[24]	尚书. 不同覆膜处理对黄土高原半干旱区农田生态系统中硝化和反硝化微生物群落结构的影响[D]. 兰州大学, 2012.SHANG Shu. (2012). Effects on community structure of ammonia oxidizers and denitrifying bacteria from different mulch treatment in semi-arid Loess Plateau, China[D].Master Dissertation. Lanzhou University, Lanzhou. (In Chinese)
[25]	Masui, A., Ikawa, S., Fujiwara, N., & Hirai, H. (2011). Influence for soil environment by continuing use of biodegradable plastic. Journal of Polymers and the Environment,19(3): 622-627.
[26]	Kapanen, A., Schettini, E., Vox, G, et al. (2008). Performance and environmental impact of biodegradable films in agriculture: a field study on protected cultivation. Journal of Polymers and the Environment, 16(2):109-122.

Responses of Soil Ammonia Oxidizers and Denitrifiers to Poly (Butylene Adipate-Co-Terephthalate) -Degrading Mulch

聚对苯二甲酸-己二酸丁二酯生物降解膜对土壤氨氧化和反硝化微生物变化的影响

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