]*>","")" /> Detection of Mrna Gene Expression of BⅢB4 in Fine-wool Sheep Skin by Semi-quantitative RT-PCR Method
Xinjiang Agricultural Sciences

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Detection of Mrna Gene Expression of BⅢB4 in Fine-wool Sheep Skin by Semi-quantitative RT-PCR Method

LAO Quan-heng;HUANG Xi-xia;TIAN Ke-chuan;XU Xin-ming;HE San-gang;ZHANG Ting-hu;WU Wei-wei;LIU Zhen   

  • Received:2009-02-25 Revised:2009-02-25 Online:2009-02-25 Published:2009-02-25

用半定量RT-PCR法检测BⅢB4基因在绵羊皮肤组织中的mRNA表达

劳荃蘅;黄锡霞;田可川;徐新明;贺三刚;张廷虎;吴伟伟;刘祯   

  1. 新疆农业大学动物科学学院,乌鲁木齐,830052;新疆畜牧科学院,乌鲁木齐,830000





摘要： 利用半定量逆转录-聚合酶链式反应(RT-PCR)技术,测定新吉细毛羊皮肤组织中 BⅢB4基因mRNA表达水平,以18SrRNA为内标,分析相对表达量及其与羊毛细度的关系.通过探讨和优化PCR体系中退火温度、MgCl2浓度和循环次数等参数,建立了检测羊毛细度相关基因mRNA表达的半定量RT-PCR体系.用Independent Samples T Test分析BⅢB4 PCR产物与18SrRNA PCR产物电泳后的灰度比值,可知BⅢB4基因mRNA表达量对羊毛细度的影响.