Effects of radiation on metabolic activities of Aureobasidium melanogenum based on biolog FF system

doi:10.6048/j.issn.1001-4330.2023.08.027

Abstract

Abstract:

【Objective】 In order to analyze the effects of radiation on the metabolic activity of Aureobasidium melanogenum, this project aims to explore the mechanism of fungal radiation tolerance.【Methods】 The experiment used ⁶⁰Co γ radiation, and set four radiation doses of 0, 2,500, 5,000, and 10,000 Gy.Biolog FF technology was used to detect the use of carbon source in FF microplate by A.melanogenum under different radiation doses, and analyze the impact on carbon metabolic activity of A.melanogenumby radiation.【Results】 There were significant differences in AWCD values under different radiation doses.With the increase of radiation dose, the metabolic activity of cells decreased significantly.The utilization of carboxylic acids and amino acids showed a downward trend, while the utilization rates of carbohydrates an upward trend.At the same time, as the radiation dose increased, the utilization of carbon source changed.Among the 95 carbon sources in the Biolog FF microplates, a total of 46 carbon sources were used, and 7 carbon sources increased in average utilization rate with the increase of radiation dose.Among them, the utilization rate of D-ribose, sucrose, D-arabinose and L-arabinose was the most obvious.【Conclusion】 Radiation can obviously reduce the metabolic activity of A.melanogenum, and the utilization of carbon source will change significantly under high doses of radiation, which may be an effective mechanism for its adaptation to radiation.

Key words: biolog FF system; Aureobasidium melanogenum; radiation; metabolic activity

摘要：

【目的】分析辐射对短梗霉代谢活性的影响,研究真菌耐辐射机理机制。【方法】分离鉴定一株产黑色素短梗霉,并进行菌株的⁶⁰Co γ射线照射试验。根据菌株的耐辐射能力,设置⁶⁰Co γ射线0、2 500、5 000和10 000 Gy 4个辐射剂量,采用Biolog FF 技术检测不同辐射下菌株对FF微平板中碳源的利用程度,分析辐射对产黑色素短梗霉代谢碳源活性的影响。【结果】不同辐射剂量下菌株AWCD值存在明显差异。随着辐射剂量的升高,细胞代谢活性明显下降,羧酸类和氨基酸的利用呈下降趋势,而碳水化合物的利用率呈上升趋势。随着辐射剂量增加,菌株对碳源的利用率发生了变化。在Biolog FF微平板95种碳源中,共有46个碳源被利用,其中7种碳源利用率随辐射剂量上升而增加。其中,D-核糖,蔗糖,D-阿拉伯糖,L-阿拉伯糖的利用率增加最为明显。【结论】辐射能明显降低产黑色素短梗霉的代谢活性,其在高剂量辐射下碳源利用类型会发生明显的改变,可能是该菌辐射应激和适应的一种有效机制。

关键词: Biolog FF 技术, 产黑色素短梗霉, 辐射, 代谢活性

CLC Number:

S188

ZHU Jing, ZHANG Zhidong, TANG Qiyong, GU Meiying. Effects of radiation on metabolic activities of Aureobasidium melanogenum based on biolog FF system[J]. Xinjiang Agricultural Sciences, 2023, 60(8): 2046-2054.

朱静, 张志东, 唐琦勇, 顾美英. 基于Biolog FF技术解析辐射对产黑色素短梗霉代谢活性的影响[J]. 新疆农业科学, 2023, 60(8): 2046-2054.

Figures/Tables 9

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辐射剂量Radiation Dose(Gy)
0 Gy		2 500 Gy		5 000 Gy		10 000 Gy
碳源 Carbon source	吸光值 Absorbance (Ci -R)	碳源 Carbon source	吸光值 Absorbance (Ci -R)	碳源 Carbon source	吸光值 Absorbance (Ci -R)	碳源 Carbon source	吸光值 Absorbance (Ci -R)
L-苹果酸 L-Malic Acid	0.716	熊果苷 Arbutin	0.608	熊果苷 Arbutin	0.562	D-核糖 D-Ribose	0.188
反丁烯二酸 Fumaric Acid	0.656	反丁烯二酸 Fumaric Acid	0.555	D-木糖 D-Xylose	0.391	蔗糖 Sucrose	0.138
琥珀酸 Succinic Acid	0.609	L-脯氨酸 L-Proline	0.537	D-蜜三糖 D-Raffinose	0.242	D-阿拉伯糖 D-Arabinose	0.126
L-谷氨酸 L-Glutamic Acidine	0.602	L-苹果酸 L-Malic Acid	0.497	6-O-D-吡喃葡萄糖酰-D-呋喃果糖 Palatinose	0.239	D-海藻糖 D-Trehalose	0.126
L-脯氨酸 L-Prol	0.597	琥珀酸 Succinic Acid	0.444	D-松三糖 D-Melezitose	0.232	熊果苷 Arbutin	0.106
熊果苷 Arbutin	0.576	水杨苷 Salicin	0.402	糊精 Dextrin	0.217	D-木糖 D-Xylose	0.105
6-O-D-吡喃葡萄糖酰-D-呋喃果糖 Palatinose	0.531	D-木糖 D-Xylose	0.389	溴代琥珀酸 Bromosuccinic Acid	0.215	L-阿拉伯糖 L-Arabinose	0.101
水杨苷 Salicin	0.481	糊精 Dextrin	0.37	D-果糖 D-Fructose	0.197
D-葡萄醛酸 D-Glucuronic Acid	0.476	L-谷氨酸 L-Glutamic Acid	0.367	D-海藻糖 D-Trehalose	0.195
D-木糖 D-Xylose	0.468	海藻糖 D-Trehalose	0.336	琥珀酸甲基酯 Succinic Acid Mono-Methyl Ester	0.192

辐射剂量Radiation Dose(Gy)
0 Gy		2 500 Gy		5 000 Gy		10 000 Gy
碳源 Carbon source	吸光值 Absorbance (Ci -R)	碳源 Carbon source	吸光值 Absorbance (Ci -R)	碳源 Carbon source	吸光值 Absorbance (Ci -R)	碳源 Carbon source	吸光值 Absorbance (Ci -R)
L-苹果酸 L-Malic Acid	0.716	熊果苷 Arbutin	0.608	熊果苷 Arbutin	0.562	D-核糖 D-Ribose	0.188
反丁烯二酸 Fumaric Acid	0.656	反丁烯二酸 Fumaric Acid	0.555	D-木糖 D-Xylose	0.391	蔗糖 Sucrose	0.138
琥珀酸 Succinic Acid	0.609	L-脯氨酸 L-Proline	0.537	D-蜜三糖 D-Raffinose	0.242	D-阿拉伯糖 D-Arabinose	0.126
L-谷氨酸 L-Glutamic Acidine	0.602	L-苹果酸 L-Malic Acid	0.497	6-O-D-吡喃葡萄糖酰-D-呋喃果糖 Palatinose	0.239	D-海藻糖 D-Trehalose	0.126
L-脯氨酸 L-Prol	0.597	琥珀酸 Succinic Acid	0.444	D-松三糖 D-Melezitose	0.232	熊果苷 Arbutin	0.106
熊果苷 Arbutin	0.576	水杨苷 Salicin	0.402	糊精 Dextrin	0.217	D-木糖 D-Xylose	0.105
6-O-D-吡喃葡萄糖酰-D-呋喃果糖 Palatinose	0.531	D-木糖 D-Xylose	0.389	溴代琥珀酸 Bromosuccinic Acid	0.215	L-阿拉伯糖 L-Arabinose	0.101
水杨苷 Salicin	0.481	糊精 Dextrin	0.37	D-果糖 D-Fructose	0.197
D-葡萄醛酸 D-Glucuronic Acid	0.476	L-谷氨酸 L-Glutamic Acid	0.367	D-海藻糖 D-Trehalose	0.195
D-木糖 D-Xylose	0.468	海藻糖 D-Trehalose	0.336	琥珀酸甲基酯 Succinic Acid Mono-Methyl Ester	0.192