Xinjiang Agricultural Sciences

Xinjiang Agricultural Sciences ›› 2023, Vol. 60 ›› Issue (3): 683-688.DOI: 10.6048/j.issn.1001-4330.2023.03.019

• Plant Protection·Facility Agriculture·Agricultural Product Processing Engineering·Microbes • Previous Articles     Next Articles

Evaluation of Rapid Detection Method for Carbofuran Residues in Apples

TIAN Feng1,2(), BAO Dongdong2, XU Zhiming2, YANG Jing2, LI Juan2, LIU Xiaohong2, TAN Huilin2(), ZHANG Zhidong1,2()   

  1. 1. Xinjiang Key Laboratory of Special Environmental Microbiology/ Institute of Applied Microbiology, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China
    2. Food Safety Testing Center of Aksu Prefecture, Aksu Xinjiang 843000, China
  • Received:2022-08-20 Online:2023-03-20 Published:2023-04-18
  • Correspondence author: TAN Huilin(1977-),female,Native place:Urumqi, Xinjiang. Research Professor professial,research field: food safety inspection,(E-mail)45118707@qq.com;
    ZHANG Zhidong (1977-),male,Native place:Yongcheng,Henan.Research Professor,research field: Exploration and utilization of microbial resources from special environments in Xinjiang,(E-mail)zhangzheedong@qq.com
  • Supported by:
    National Key R & D Program Project(2018YFC1603400);Talent Project of Aksu Prefecture 2020;XPCC Project of Tackling Hard-Nut Problems in Science and Technology(2018AB042);Team Building Special Fund for Independent Cultivation Program of Xinjiang Academy of Agricultural Sciences(nkyzztd-001)

苹果中克百威残留快检测定及方法评估

田锋1,2(), 包东东2, 徐智明2, 杨婧2, 李娟2, 刘晓红2, 谭慧林2(), 张志东1,2()   

  1. 1.新疆农业科学院微生物应用研究所/新疆特殊环境微生物实验室,乌鲁木齐 830091
    2.阿克苏地区食品安全检测中心,新疆阿克苏 843000
  • 通讯作者: 谭慧林(1977-),女,新疆乌鲁木齐人,正高级工程师,研究方向为食品安全检验,(E-mail)45118707@qq.com;
    张志东(1977-),男,新疆乌鲁木齐人,研究员,博士,硕士生导师,研究方向为特殊环境微生物及食品微生物学,(E-mail)zhangzheedong@qq.com
  • 作者简介:田锋(1990-),男,新疆阿克苏人,工程师,研究方向为食品安全检验,(E-mail)121745510@qq.com
  • 基金资助:
    国家重点研发计划(2018YFC1603400);阿克苏地区2020年度托峰人才项目;兵团科技攻关项目(2018AB042);新疆农业科学院自主培育项目团队建设专项(nkyzztd-001)

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Abstract:

【Objective】 To evaluate the adaptability of different rapid detection kits in detecting carbofuran residues in apples, improve the sensitivity and accuracy of the methods and ensure the efficiency and accuracy of selective check in apple products.【Methods】 In this study, apples from six local markets in Aksu were taken as test samples, and the liquid chromatography of industry standard NY/T 761-2008 Part 3 was used as reference method.Three rapid detection kits were selected respectively, and their adaptability for rapid detection was evaluated according to Technical Specifications for Evaluation of Rapid Detection Methods of Food.【Results】 None of carbofuran residues in all samples was detected by the reference method and rapid detection kits.Results in different spiked levels showed that positive color could be observed above 0.01 mg/kg, which was lower than the maximum residue limits for pesticides in food listed in GB/T 2763-2019.All rapid detection kits displayed that the sensitivity was 100%, and specificity for carbofuran residues in apples was 50%.Compared with instructions, false negative rate was 0, false positive rates were 50% and relative accuracy was 90%.【Conclusion】 The rapid detection kits can be used in fast testing and screening of carbofuran residues in apples.

Key words: carbofuran; apples; rapid detection kit; evaluate

摘要：

【目的】提高苹果中克百威残留快速检测方法的灵敏度与准确度,确保苹果产品中克百威农药残留抽查的高效和准确性。【方法】以新疆阿克苏当地6个点位市售苹果为检测样品,采用行业标准NY/T 761-2008第3部分的液相色谱法为参比方法,分别选择3家快检试剂盒,根据《食品快速检测方法评价技术规范》对其快检适应性进行评估。【结果】利用参比方法和快检试剂盒均未从苹果样品中检出克百威残留。3家快检试剂盒可在0.01 mg/kg以上呈阳性显色反应,低于GB/T 2763-2019《食品安全国家标准食品中农药最大残留限量》中的限量要求;试剂盒灵敏度均为100%、特异性均为50%、假阴性率均为0、假阳性率均为50%、相对准确度均为90%。【结论】所测试剂盒适用于苹果中克百威残留量的快速筛查。

关键词: 苹果, 克百威, 快检试剂盒, 评估

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