Investigation of Grazing Sheep Brucellosis and Implementation of Prevention Measures

doi:10.6048/j.issn.1001-4330.2023.02.027

Abstract

Abstract:

【Objective】 Investigation of brucellosis (brucellosis) in grazing sheep, the applicability analysis of testing methods, and the implementation of prevention and control measures. 【Methods】 90 serum samples from aborted sheep were detected by RBT, GICA, SAT, MAT, FPA, iELISA, cELISA and PCR, and the applicability characteristics of the diagnostic method and the results of the field investigation were used for statistical analysis, and control measures were proposed. 【Results】 In 90 sheep serum samples, positive RBT, GICA, SAT, MAT, FPA, iELISA and cELISA detection rates were 8.89%(8/90), 12.22%(11/90), 17.78%(16/90), 14.44%(13/90), 21.11%(19/90), 25.56%(23/90), 24.44%(22/90) and 14 positive samples by PCR. 【Conclusion】 High sensitivity methods were used for initial screening and strong specificity method were used for diagnosis in clinical practice.To be more specific, RBT, GICA, FPA or iELISA could be used for initial screening, and SAT, MAT or cELISA for diagnosis.The test results showed that the positive rate of grazing sheep increased with age (adult sheep 42% > weaning lamb 2.5%).A higher positive rate were detected in females (29.68%) than in males 11.54trading and slaughter were allowed%).Sheep with miscarriage history had a higher risk of brucellosis and adult females were more susceptible to brucella.The positive sheep were treated innocuously, the sheep shed and the surrounding environment were thoroughly disinfected, the negative sheep were immunized using the brucellosis M5-90 weak toxicity vaccine, and two months after immunization they were allowed to be slaughtered or for trading.

Key words: grazing sheep; brucellosis; serological testing method; PCR method

摘要：

【目的】 研究一起放牧羊群布鲁氏菌病(布病)分析检测方法适用性,为防治措施提供依据。【方法】 采用RBT、GICA、SAT、MAT、FPA、iELISA、cELISA和PCR等方法检测90份来自流产羊群的血清样品,并从诊断方法的适用性特点、现场调查的结果进行统计分析。【结果】 在90份羊血清样品中,RBT、GICA、SAT、MAT、FPA、iELISA、cELISA的阳性检出率分别为:8.89%(8/90)、12.22%(11/90)、17.78%(16/90)、14.44%(13/90)、21.11%(19/90)、25.56%(23/90)、24.44%(22/90),PCR检测出14份阳性。【结论】 初筛可选用RBT、GICA、FPA或iELISA等方法,确诊选用SAT、MAT或cELISA等方法。放牧羊群布病阳性率随年龄增加呈上升趋势(成年羊42%>断奶羔羊2.5%);雌性羊布病阳性率高于雄性(雌性29.68%>雄性11.54%),有流产史的羊群患布病的风险更高,成年雌性羊更易感染布鲁氏菌。检出阳性羊进行无害化处理,羊舍和周围环境进行彻底消毒,使用布病M5-90弱毒疫苗对阴性羊进行免疫,免疫2个月后允许交易和屠宰。

关键词: 放牧羊, 布鲁氏菌病, 血清学检测方法, PCR方法

CLC Number:

S855

JIN Xiaoye, YE Feng, LIU Liya, MA Xiaojing, LI Xin, SHU Zhan, CHEN Zhuo, ZHONG Qi. Investigation of Grazing Sheep Brucellosis and Implementation of Prevention Measures[J]. Xinjiang Agricultural Sciences, 2023, 60(2): 479-484.

金肖叶, 叶锋, 刘丽娅, 马晓菁, 李鑫, 舒展, 陈卓, 钟旗. 一起放牧羊群布鲁氏菌病的分析检测[J]. 新疆农业科学, 2023, 60(2): 479-484.

Figures/Tables 9

References 35

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		金标准 Gold standard				合计 Amount to
		+(份)		-(份)		合计 Amount to
对照方法检测结果 Results of the control method test	+(份)		a		b		a+b(r₁)
对照方法检测结果 Results of the control method test	-(份)		c		d		c+d(r₂)
合计Amount to		a+c(e₁)		b+d(e₂)		n

		金标准 Gold standard				合计 Amount to
		+(份)		-(份)		合计 Amount to
对照方法检测结果 Results of the control method test	+(份)		a		b		a+b(r₁)
对照方法检测结果 Results of the control method test	-(份)		c		d		c+d(r₂)
合计Amount to		a+c(e₁)		b+d(e₂)		n

		RBT		假阳性率 FPR^a (%)	假阴性率 FNR^b	敏感性 Sensi tivity	特异性 Specificity (%)
		+	-	假阳性率 FPR^a (%)	假阴性率 FNR^b	敏感性 Sensi tivity	特异性 Specificity (%)
GICA	+	8	3	3.66	0	100%	96.34
GICA	-	0	79	3.66	0	100%	96.34
FPA	+	8	11	13.41	0	100	86.59
FPA	-	0	71	13.41	0	100	86.59
iELISA	+	8	15	18.29	0	100	81.71
iELISA	-	0	67	18.29	0	100	81.71

		RBT		假阳性率 FPR^a (%)	假阴性率 FNR^b	敏感性 Sensi tivity	特异性 Specificity (%)
		+	-	假阳性率 FPR^a (%)	假阴性率 FNR^b	敏感性 Sensi tivity	特异性 Specificity (%)
GICA	+	8	3	3.66	0	100%	96.34
GICA	-	0	79	3.66	0	100%	96.34
FPA	+	8	11	13.41	0	100	86.59
FPA	-	0	71	13.41	0	100	86.59
iELISA	+	8	15	18.29	0	100	81.71
iELISA	-	0	67	18.29	0	100	81.71

检测方法 Tests	符合率 Agreement rate(%)	Kappa值 Kappa value
RBT vs GICA	96.67	0.824
RBT vs FPA	87.78	0.534
RBT vs iELISA	83.33	0.443
GICA vs FPA	91.11	0.684
GICA vs iELISA	86.66	0.577
FPA vs iELISA	86.67	0.628