Integrated Taxonomy Studies on Cleroclytus (Coleoptera: Cerambycidae) in China

doi:10.6048/j.issn.1001-4330.2022.04.021

Abstract

Abstract:

【Objective】 To study the classification of the genus: Cleroclytus collaris Jakovlev,1885 and Cleroclytus strigicollis Jakovlev,1900 by combining DNA barcoding technology and morphological characteristics in the hope of providing a research basis for its accurate identification and a reference for the related identification of this genus in the future.【Methods】 The structure of ovipositor and internal genitalia was described by dissecting the female adult genitalia.Genomic DNA was extracted from the head and throx of the adult, and mitochondrial cytochrome oxidase I (COI) was amplified by PCR with universal primers to construct the DNA barcodes.【Result】 The results of morphology and genitalia structure showed that there was no significant morphological difference between the two species.The results of DNA barcoding showed that the COI gene sequence length was about 670bp.The sequence similarity between Cleroclytus collaris and Cleroclytus strigicollis was 99.60%.The average genetic distance between them was 0.6%, which proved that they were the same species, which was basically consistent with the results of morphological identification.【Conclusion】 DNA barcoding technology and morphological characteristics are combined to determine the classification of two species of Cleroclytus in Xinjiang, confirming that they are synonyms.

Key words: COI gene sequence; female genitalia; species identification; comparative morphology; integrated taxonomy

摘要：

【目的】结合DNA条形码技术与形态学比较,研究并分类克虎天牛属Cleroclytus两近似种新疆克虎天牛Cleroclytus collaris Jakovlev,1885和沟胸克虎天牛Cleroclytus strigicollis Jakovlev,1900,为其准确鉴定提供研究依据。【方法】采用体视显微镜,观察比较克虎天牛的外部形态特征;解剖克虎天牛雌性产卵器和内生殖器,描述其结构特点;从克虎天牛头部和胸部组织中提取基因组DNA,以通用引物进行PCR扩增线粒体细胞色素氧化酶I (Cytochrome Oxidase I, COI),构建DNA条形码。【结果】新疆克虎天牛与沟胸克虎天牛均未发现明显形态差异;克虎天牛的COI基因序列长度约为670 bp,新疆克虎天牛与沟胸克虎天牛序列相似性达到99.60%,二者的平均遗传距离是0.6%。【结论】新疆克虎天牛与沟胸克虎天牛为同一种,与形态学鉴定结果基本相吻合。沟胸克虎天牛是新疆克虎天牛的异名。

关键词: COI基因序列, 雌性外生殖器, 物种鉴定, 比较形态学, 整合分类学

CLC Number:

S763.3

GUO Shuhan, LUO Shungang, ZHONG Wen, KANG Ning, HU Hongying. Integrated Taxonomy Studies on Cleroclytus (Coleoptera: Cerambycidae) in China[J]. Xinjiang Agricultural Sciences, 2022, 59(4): 957-965.

郭书含, 罗顺刚, 钟问, 康宁, 胡红英. 中国克虎天牛属整合分类[J]. 新疆农业科学, 2022, 59(4): 957-965.

Figures/Tables 13

References 25

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标本编号 Specimen number	种名 Species	采集时间 Collectingdate	采集地点 Collectingsites	保存方法 Preservation methods
1	新疆克虎天牛 C.collaris	2017.7.19	新疆伊犁哈萨克自治州新源县阿勒玛乡改良场植物园	干标本室温保存
2	新疆克虎天牛 C.collaris	2017.7.19	新疆伊犁哈萨克自治州新源县阿勒玛乡改良场植物园	干标本室温保存
3	新疆克虎天牛 C.collaris	2017.7.19	新疆伊犁哈萨克自治州新源县阿勒玛乡改良场植物园	干标本室温保存
4	沟胸克虎天牛 C.strigicollis	2020.6.15	新疆乌鲁木齐市新疆大学校内	无水乙醇-20℃冷藏
5	沟胸克虎天牛 C.strigicollis	2020.6.15	新疆乌鲁木齐市新疆大学校内	无水乙醇-20℃冷藏
6	沟胸克虎天牛 C.strigicollis	2020.6.15	新疆乌鲁木齐市新疆大学校内	无水乙醇-20℃冷藏

标本编号 Specimen number	种名 Species	采集时间 Collectingdate	采集地点 Collectingsites	保存方法 Preservation methods
1	新疆克虎天牛 C.collaris	2017.7.19	新疆伊犁哈萨克自治州新源县阿勒玛乡改良场植物园	干标本室温保存
2	新疆克虎天牛 C.collaris	2017.7.19	新疆伊犁哈萨克自治州新源县阿勒玛乡改良场植物园	干标本室温保存
3	新疆克虎天牛 C.collaris	2017.7.19	新疆伊犁哈萨克自治州新源县阿勒玛乡改良场植物园	干标本室温保存
4	沟胸克虎天牛 C.strigicollis	2020.6.15	新疆乌鲁木齐市新疆大学校内	无水乙醇-20℃冷藏
5	沟胸克虎天牛 C.strigicollis	2020.6.15	新疆乌鲁木齐市新疆大学校内	无水乙醇-20℃冷藏
6	沟胸克虎天牛 C.strigicollis	2020.6.15	新疆乌鲁木齐市新疆大学校内	无水乙醇-20℃冷藏

种名 Species	GenBank登录号 GenBank login number
咖啡脊虎天牛 Xylotrechus grayii	NC 030782
暗褐断眼天牛1 Tetropium Fuscum	AY 389477
暗褐断眼天牛2 Tetropium Fuscum	AY 389478

种名 Species	GenBank登录号 GenBank login number
咖啡脊虎天牛 Xylotrechus grayii	NC 030782
暗褐断眼天牛1 Tetropium Fuscum	AY 389477
暗褐断眼天牛2 Tetropium Fuscum	AY 389478

形态特征 Traditional morphologal characters	新疆克虎天牛 C.collaris	沟胸克虎天牛 C.strigicollis
头部 Head	褐红色,向前倾斜	褐红色,向前倾斜
触角 Antenna	11节,第1节膨大	11节,第1节膨大
触角长与体长比 Ratio of antenna length to body length	达到鞘翅末端	不达鞘翅末端
前胸背板体色 Color of pronotum	深褐色,基部有白色直立细长白毛	褐红色,基部有白色直立细长白毛
前胸背板刻纹 Sculpture of pronotum	纵沟纹和刻点,在基半部刻点比纵沟纹占优势	刻纹明显,刻点少量分布于后部
小盾片 Scutellum	半圆形,覆有白毛,下方和两侧略下凹	半圆形,覆有白毛,下方和两侧略下凹
鞘翅基部 The base of elytron	具白色的短斜脊	具白色的短斜脊
鞘翅前端 The front of elytron	红褐色,前三分之一有一微隆起的呈乳黄色而光滑的斑纹	红褐色,前三分之一有一微隆起的呈乳黄色而光滑的斑纹
鞘翅后端 The back of elytron	深褐色	黑褐色
足 Legs	褐红色	褐红色