Split-Plot Design for Optimizing Compatibility of Biological Pesticide with Melanin and Effect on Cotton Yield

doi:10.6048/j.issn.1001-4330.2018.09.019

Xinjiang Agricultural Sciences ›› 2018, Vol. 55 ›› Issue (9): 1728-1736.DOI: 10.6048/j.issn.1001-4330.2018.09.019

Split-Plot Design for Optimizing Compatibility of Biological Pesticide with Melanin and Effect on Cotton Yield

ZHU Jing¹, ZHANG Zhi-dong¹, LIU Xiao-jing², TANG Qi-yong¹, WANG Bo¹, GU Mei-ying¹, SONG Su-qin¹, WANG Wei¹, ZHANG Li-juan¹

1.Research Institute of Applied Microbiology, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China;
2. College of Food Sciences and Pharmaceutical Sciences, Xinjiang Agricultural University, Urumqi 830052, China

Received:2018-07-23 Online:2018-09-20 Published:2018-12-07
Correspondence author: ZHANG Zhi-dong(1977-),male,native place: Shi He Zi, Xinjiang. Professor, research field: Special environmental microbiology, (E-mail)zhangzheedong@sohu.com
Supported by:
Xinjiang High Technology R and D Program "Study on Fermentation Technology of Natural Melanin and Its Application in Biological Pesticide" (201511105)

黑色素与生物农药配伍优化及其对棉田产量的影响

朱静¹, 张志东¹, 刘晓静², 唐琦勇¹, 王博¹, 顾美英¹, 宋素琴¹, 王玮¹, 张丽娟¹

1.新疆农业科学院微生物应用研究所,乌鲁木齐 830091;
2.新疆农业大学食品科学与药学学院,乌鲁木齐 830052

通讯作者: 张志东(1977-),男,新疆石河子人,硕士,研究员,研究方向为特殊环境微生物学,(E-mail)zhangzheedong@sohu.com
作者简介:朱静(1981-),女,云南昆明人,硕士,副研究员,研究方向为特殊环境微生物学,(E-mail)122543537@qq.com
基金资助:
新疆维吾尔自治区高技术研究发展计划“天然黑色素高产菌发酵工艺及其在生物农药中的应用研究”(201511105)

Abstract

Abstract: 【Objective】 To investigate the photoprotective effect of natural melanin on biological pesticides, promote efficiency and decrease consumption of biological pesticides. 【Method】Melanin broth was obtained by fermentation of Aureobasidium pullulans MF1. Under plot experiment conditions, a split-plot design was conducted to optimize compatibility with the concentration of biological pesticides as the main treatment and melanin concentration as the secondary treatment, and the effect on cotton yield was analyzed.【Result】When the dosage was optimized as follows: Bt 25 g, avermectin 5 mL, propoxybromide 12.5 g and melanin 2.5 g per mu, the maximum killing rate of aphid and the highest yield of cotton were obtained with 97.7% and 14.3 kg per plot, respectively. The results were significantly higher than that of the control plot. 【Conclusion】Melanin broth fermented by MF1 can not only decrease consumption of biological pesticides, but also increase the yield of cotton. It has provided scientific basis for further application of natural melanin in biological pesticides.

Key words: melanin; Aureobasidium pullulans; photoprotective additives; split-plot design ; ;

摘要： 【目的】研究天然黑色素对生物农药的光保护作用,提高生物农药药效,降低农药使用量。为推广天然黑色素在生物农药中的应用提供科学依据。【方法】利用出芽短梗霉MF1发酵获得天然黑色素,在农田小区试验条件下,以生物农药浓度为主处理,黑色素浓度为副处理的裂区试验,确定其最佳配伍,分析其对棉田产量的影响。【结果】当每667 m²棉田使用Bt 25 g、阿维菌素5 mL、甲维丙溴磷12.5 g,黑色素2.5 g时,蚜虫杀死率最大可达到97.7%,最大棉花产量可达14.3 kg/小区,显著高于对照小区。【结论】添加天然黑色素不仅可降低生物农药的使用量,同时具有提效增产的作用。

关键词: 黑色素, 出芽短梗霉, 光保护助剂, 裂区试验

CLC Number:

S14-3

ZHU Jing, ZHANG Zhi-dong, LIU Xiao-jing, TANG Qi-yong, WANG Bo, GU Mei-ying, SONG Su-qin, WANG Wei, ZHANG Li-juan. Split-Plot Design for Optimizing Compatibility of Biological Pesticide with Melanin and Effect on Cotton Yield[J]. Xinjiang Agricultural Sciences, 2018, 55(9): 1728-1736.

朱静, 张志东, 刘晓静, 唐琦勇, 王博, 顾美英, 宋素琴, 王玮, 张丽娟. 黑色素与生物农药配伍优化及其对棉田产量的影响[J]. 新疆农业科学, 2018, 55(9): 1728-1736.

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Split-Plot Design for Optimizing Compatibility of Biological Pesticide with Melanin and Effect on Cotton Yield

黑色素与生物农药配伍优化及其对棉田产量的影响

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