Abstract: 【Objective】 FeCl₃ solution causes the accumulation of resveratrol, but the mechanism is not clear. Here we aim to study the relationship between the Halliwell-Asada (H-A) pathway and resveratrol accumulation from the perspective of damage protection.【Method】After FeCl₃ solution treatment of grape leaves, the degree of cell damage and the level of reactive oxygen species were detected, and the expression of resveratrol and H-A pathway related genes were analyzed.【Result】The results showed that the oxidative stress level of leaves treated with FeCl₃ solution were positively correlated with the damage of foliar tissue. A significant resveratrol accumulation was also observed. In addition, the maximum content of resveratrol reached 36.83 μg/g after treatment in a dose-dependant manner. The gene expression levels of resveratrol metabolic enzymes and key enzymes in H-A pathway were increased in FeCl₃ treatment, such as PAL, C4H, 4CL, STS, APX and MDAR except DHAR. Resveratrol contents in H₂O₂ co-treatment were raised, contrary to co-treatments of antioxidant reagents NAC and GSH-EE.【Conclusion】FeCl₃ solution decreased ROS scavenging capability of H-A pathway, thus causing oxidative stress and leading to resveratrol accumulation in grapevine samples, which underlines a correlation between resveratrol metabolism and H-A pathway.

Key words: resveratrol; foliar injury; H-A pathway; ROS accumulation; Vitis vinifera L. cv. Red Globe

摘要： 【目的】FeCl₃溶液可导致葡萄白藜芦醇积累但机制不明,研究Halliwell-Asada(H-A)途径与白藜芦醇积累的关系在损伤保护角度。【方法】FeCl₃溶液处理葡萄叶片后,检测细胞损伤程度和活性氧水平,分析白藜芦醇与H-A途径相关基因表达变化规律。【结果】FeCl₃溶液造成叶片活性氧积累与叶片损伤正相关,且伴随白藜芦醇呈量效关系积累,峰值36.83 μg/g;白藜芦醇合成途径以及H-A途径下相关酶PAL、C4H、4CL、STS、APX和MDAR的基因表达量除DHAR基因外均明显上调;H₂O₂协同处理增加白藜芦醇含量,抗氧化剂NAC和GSH-EE协同则相反。【结论】FeCl₃溶液降低葡萄叶片H-A途径对活性氧的清除能力,造成氧化胁迫并引起白藜芦醇积累,提示白藜芦醇合成与H-A途径存在偶联。

关键词: 白藜芦醇, 细胞损伤, H-A途径, ROS积累, 美国红地球葡萄