4种沙漠植物固氮菌的分离与固氮活性检测

doi:10.6048/j.issn.1001-4330.2024.11.015

新疆农业科学 ›› 2024, Vol. 61 ›› Issue (11): 2742-2749.DOI: 10.6048/j.issn.1001-4330.2024.11.015

• 作物遗传育种·耕作栽培 • 上一篇下一篇

4种沙漠植物固氮菌的分离与固氮活性检测

王丽君¹(), 孙蓓蓓¹, 王春燕², 夏占峰², 马国财¹()

1.塔里木大学分析测试中心,新疆阿拉尔 843300
2.塔里木大学生命科学与技术学院,新疆阿拉尔 843300

收稿日期:2024-05-27 出版日期:2024-11-20 发布日期:2025-01-08
通信作者: 马国财(1978-),男,辽宁朝阳人,高级实验师,研究方向为微区分析,(E-mail)18845420@qq.com
作者简介:王丽君(1981-),女,山东聊城人,高级实验师,研究方向为微生物资源,(E-mail)1489968232@qq.com
基金资助:
新疆生产建设兵团财政科技计划项目“塔里木盆地来源微生物防治新疆主要农林病害菌剂的开发和应用”(2021BC009);新疆综合科学考察项目子课题“特殊环境微生物种质资源调查与收集”(2022xjkk150307);国家级大学生创新创业训练计划项目“基于塔里木盆地高抑菌活性放线菌发酵产物的生物农药创制”(202210757018)

Isolation and nitrogen-fixing activity detection of bacteria from four desert plants

WANG Lijun¹(), SUN Beibei¹, WANG Chunyan², XIA Zhanfeng², MA Guocai¹()

1. Analysis and Testing Center, Tarim University, Aral Xinjiang 843300,China
2. College of Life Science and Technology, Tarim University, Aral Xinjiang 843300,China

Received:2024-05-27 Published:2024-11-20 Online:2025-01-08
Supported by:
Financial and Technological Projects of the Xinjiang Production and Construction Corps "Development and application of microbial agents from the Tarim Basin for the prevention and control of major agricultural and forest diseases in Xinjiang″(2021BC009);Sub-project of the Xinjiang Comprehensive Scientific Investigation Project "Investigation and collection of microbial germplasm resources in special environments"(2022xjkk150307);National College Students' Innovation and Entrepreneurship Training Program "Development of biopesticides based on the fermentation products of high inhibitory active actinomycetes from the Tarim Basin"(202210757018)

摘要/Abstract

摘要：

【目的】研究塔克拉玛干沙漠植物获取氮素营养的途径,为塔克拉玛干沙漠固氮菌资源的开发和干旱区生态环境的保护提供支持。【方法】以沙漠典型豆科植物苦豆子、柽柳科植物多枝柽柳、菊科植物花花柴和蓼子朴为研究对象,利用YMA(酵母甘露醇琼脂)和Ashby(阿须贝)无氮源培养基从植物根部分离固氮菌。【结果】从苦豆子样品分离获得19株菌,分布于11个属;从花花柴样品分离获得8株菌,分布于5个属;从蓼子朴样品分离获得5株菌,分布于5个属;从多枝柽柳样品分离获得4株菌,分布于2个属。YMA培养基分离获得28株菌,分布于15个属;Ashby无氮培养基分离获得8株菌,分布于7个属。10株菌有固氮活性,从多枝柽柳中获得的Rhizobium sp. 80417、从苦豆子中获得的Enterobacter sp.81227和Acinetobacter sp.81240、从花花柴中获得的Paenibacillus sp.80802 和Microbacterium sp.80803具有较强的固氮能力。【结论】固氮菌种类的多样化,促进了营养贫瘠环境氮元素的补充。

关键词: 塔克拉玛干沙漠; 苦豆子; 多枝柽柳; 花花柴; 蓼子朴; 固氮菌

Abstract:

【Objective】 In order to explore the ways for plants to obtain nitrogen nutrients in the Taklimakan Desert.【Methods】 the typical legume plants Sophora alopecuroides, Tamarix ramosissima, Karelinia caspica and Inula salsoloides were used as research materials.Nitrogen-fixing bacteria were isolated from plant roots using YMA and Ashby nitrogen-free medium.It provides support for the development of nitrogen-fixing bacteria resources in the Taklimakan Desert and the protection of the ecological environment in arid areas.【Results】 A total of 19 strains were isolated from Sophora alopecuroides samples, which were distributed in 11 genera.8 strains were isolated from Karelinia caspica samples and distributed in 5 genera.5 strains were isolated from Inula salsoloides samples and distributed in 5 genera.4 strains were isolated from Tamarix ramosissima samples and distributed in 2 genera.28 strains of bacteria were isolated from YMA medium, distributed in 15 genera; 8 strains of bacteria were isolated from Ashby nitrogen free medium and distributed in 7 genera.Rhizobium sp.80417 from Tamarix ramosissima, Enterobacter sp.81227 and Acinetobacter sp.81240 from Sophora alopecuroides, Paenibacillus sp.80802 and Microbacterium sp.80803 from karelinia caspica had strong nitrogen fixation ability.【Conclusion】 The diversity of nitrogen-fixing bacteria has promoted the supplement of nitrogen in nutrient poor environment.

Key words: Taklimakan Desert; Sophora alopecuroides; Tamarix ramosissima; Karelinia caspia; Inula salsoloides; nitrogen-fixing microorganism

中图分类号:

S188

王丽君, 孙蓓蓓, 王春燕, 夏占峰, 马国财. 4种沙漠植物固氮菌的分离与固氮活性检测[J]. 新疆农业科学, 2024, 61(11): 2742-2749.

WANG Lijun, SUN Beibei, WANG Chunyan, XIA Zhanfeng, MA Guocai. Isolation and nitrogen-fixing activity detection of bacteria from four desert plants[J]. Xinjiang Agricultural Sciences, 2024, 61(11): 2742-2749.

图/表 7

参考文献 22

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序号 Order number	菌株编号 Strain number	最相近菌株 Closest strain	16S rRNA 基因相似度 16S rRNA Gene similarity (%)		分离来源 Source of Separation		分离培养基 Isolation medium
1	80801	Microbacterium indicum BBH6	98.10		花花柴		YMA
2	80802	Paenibacillus tundrae A10b	99.36		花花柴		Ashby
3	80803	Microbacterium indicum BBH6	98.61		花花柴		Ashby
4	80805	Bacillus halotolerans ATCC 25096	99.93		花花柴		YMA
5	80806	Bacillus zhangzhouensis DW5-4	99.93		花花柴		YMA
6	80807	Paenibacillus tundra A10b	99.29		花花柴		YMA
7	80809	Nocardioides albus KCTC 9186	99.85		花花柴		Ashby
8	80811	Pantoea alhagi LTYR-11Z	99.57		花花柴		YMA
9	81202	Bacillus altitudinis 41KF2b	99.93		苦豆子		YMA
10	81203	Arthrobacter endophyticus EGI6500322	99.85		苦豆子		YMA
11	81204	Bacillus idriensis SMC 4352-2	99.79		苦豆子		YMA
12	81205	Zhihengliuella halotolerans YIM 70185	99.78		苦豆子		YMA
13	81206	Bacillus endolithicus JC267	98.78		苦豆子		YMA
14	81208	Streptomyces albidoflavus DSM 40455	99.71		苦豆子		YMA
15	81211	Bacillus litoralis SW-211	98.55		苦豆子		YMA
16	81213	Bacillus megaterium NBRC 15308	99.93		苦豆子		YMA
17	81217	Bacillus zhangzhouensis DW5-4	99.85		苦豆子		YMA
18	81218	Brevibacterium frigoritolerans DSM 8801	100.00		苦豆子		YMA
19	81221	Paenibacillus peoriae DSM 8320	99.57		苦豆子		YMA
20	81222	Bacillus endophyticus 2DT		99.79		苦豆子	YMA
21	81225	Bacillus safensis FO-36b		99.93		苦豆子	YMA
22	81227	Enterobacter xiangfangensis LMG 27195		99.71		苦豆子	Ashby
23	81228	Pantoea vagans LMG 24199		99.56		苦豆子	Ashby
24	81233	Sphingobium yanoikuyae ATCC 51230		99.63		苦豆子	YMA
25	81235	Blastomonas natatoria DSM 3183		99.85		苦豆子	YMA
26	81237	Pantoea vagans LMG 24199		99.26		苦豆子	YMA
27	81240	Acinetobacter johnsonii CIP 64.6		99.42		苦豆子	YMA
28	80402	Rhizobium tarimense PL-41		98.93		多枝柽柳	YMA
29	80405	Caulobacter vibrioides CB51		99.93		多枝柽柳	Ashby
30	80408	Rhizobium radiobacter ATCC 19358		99.25		多枝柽柳	Ashby
31	80417	Rhizobium arenae MIM27		99.85		多枝柽柳	YMA
32	80604	Promicromonospora iranensis HM 792		99.49		蓼子朴	YMA
33	80611	Massilia timonae CCUG 45783		98.12		蓼子朴	YMA
34	80612	Rhizobium pusense LMG 25623		100.00		蓼子朴	Ashby
35	80613	Serratia plymuthica DSM 4540		99.86		蓼子朴	YMA
36	80614	Pantoea agglomerans DSM 3493		99.86		蓼子朴	YMA

序号 Order number	菌株编号 Strain number	最相近菌株 Closest strain	16S rRNA 基因相似度 16S rRNA Gene similarity (%)		分离来源 Source of Separation		分离培养基 Isolation medium
1	80801	Microbacterium indicum BBH6	98.10		花花柴		YMA
2	80802	Paenibacillus tundrae A10b	99.36		花花柴		Ashby
3	80803	Microbacterium indicum BBH6	98.61		花花柴		Ashby
4	80805	Bacillus halotolerans ATCC 25096	99.93		花花柴		YMA
5	80806	Bacillus zhangzhouensis DW5-4	99.93		花花柴		YMA
6	80807	Paenibacillus tundra A10b	99.29		花花柴		YMA
7	80809	Nocardioides albus KCTC 9186	99.85		花花柴		Ashby
8	80811	Pantoea alhagi LTYR-11Z	99.57		花花柴		YMA
9	81202	Bacillus altitudinis 41KF2b	99.93		苦豆子		YMA
10	81203	Arthrobacter endophyticus EGI6500322	99.85		苦豆子		YMA
11	81204	Bacillus idriensis SMC 4352-2	99.79		苦豆子		YMA
12	81205	Zhihengliuella halotolerans YIM 70185	99.78		苦豆子		YMA
13	81206	Bacillus endolithicus JC267	98.78		苦豆子		YMA
14	81208	Streptomyces albidoflavus DSM 40455	99.71		苦豆子		YMA
15	81211	Bacillus litoralis SW-211	98.55		苦豆子		YMA
16	81213	Bacillus megaterium NBRC 15308	99.93		苦豆子		YMA
17	81217	Bacillus zhangzhouensis DW5-4	99.85		苦豆子		YMA
18	81218	Brevibacterium frigoritolerans DSM 8801	100.00		苦豆子		YMA
19	81221	Paenibacillus peoriae DSM 8320	99.57		苦豆子		YMA
20	81222	Bacillus endophyticus 2DT		99.79		苦豆子	YMA
21	81225	Bacillus safensis FO-36b		99.93		苦豆子	YMA
22	81227	Enterobacter xiangfangensis LMG 27195		99.71		苦豆子	Ashby
23	81228	Pantoea vagans LMG 24199		99.56		苦豆子	Ashby
24	81233	Sphingobium yanoikuyae ATCC 51230		99.63		苦豆子	YMA
25	81235	Blastomonas natatoria DSM 3183		99.85		苦豆子	YMA
26	81237	Pantoea vagans LMG 24199		99.26		苦豆子	YMA
27	81240	Acinetobacter johnsonii CIP 64.6		99.42		苦豆子	YMA
28	80402	Rhizobium tarimense PL-41		98.93		多枝柽柳	YMA
29	80405	Caulobacter vibrioides CB51		99.93		多枝柽柳	Ashby
30	80408	Rhizobium radiobacter ATCC 19358		99.25		多枝柽柳	Ashby
31	80417	Rhizobium arenae MIM27		99.85		多枝柽柳	YMA
32	80604	Promicromonospora iranensis HM 792		99.49		蓼子朴	YMA
33	80611	Massilia timonae CCUG 45783		98.12		蓼子朴	YMA
34	80612	Rhizobium pusense LMG 25623		100.00		蓼子朴	Ashby
35	80613	Serratia plymuthica DSM 4540		99.86		蓼子朴	YMA
36	80614	Pantoea agglomerans DSM 3493		99.86		蓼子朴	YMA

4种沙漠植物固氮菌的分离与固氮活性检测

Isolation and nitrogen-fixing activity detection of bacteria from four desert plants

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