Abstract: [Objective]For protecting and utilizing Tricholoma mongolicum Imai.,using liquid culture to grasp its nutritional characteristics,[Method]taking the Tricholoma mongolicum from Bayanbulak as the material for shake cultivation,the biomass,characteristics and culture time are studied to explain its nutritional characteristics and suitable culture condition.[Result]The result indicates that the mycelium can be cultured in the liquid medium with a certain time and the best medium formulation is corn grits 5;,glucose 2;,KH_2PO_4 0.1;,MgSO_4 0.05;,soybean 0.1; and pH 6.5～7.0. It has enough dissolved oxygen and a more stable biomass when there is 40; liquid medium in the bottle and cultured at the speed 125 r/min,the biomass is 0.684 8 g/100 mL and the mycelium age is 21 d.[Conclusion]It has a poor adaptation ability when growing both in the potato medium and the rice bran medium.