Abstract: [Objective] To obtain PgANS,PgCHS and PgACT genes in Kashgar pomegranate kernels and analyze their sequence.[Method] Xinjiang pomegranate in Kashgar was selected as the materials.The total RNA of pomegranate Kernels was extracted with Phenol-chloroform.The first cDNA was synthesized with TIANScript kit,and amplified to cDNA by RT-PCR using special primer.The pMD19-T vector linked with the cDNA fragment was transformed into Escherichia coli DH5α,and the positive clone was sequenced.[Result] The fragment of PgANS was 474bp,GenBank accession number was KT779433.Compared with Punica granatum L.which the accession numbers were KF841619,GU376749 on NCBI,the PgANS homology of nucleotide sequence was 99;.At the same time,the sequence analysis demonstrated that the deduced nucleotide sequence shared 85;,79; and 76; homology with that of Ipomoea batatas (GU598212),Morus alba (JF499385) and Fragariax ananassa (AY695817);the fragment of PgCHS was 140bp and the PgACT was 148bp.Compared with Punica granatum L.(JF747148,GU376750) on NCBI,the PgCHS homology of nucleotide sequence was 99;,and the PgACT was 93;.[Conclusion] The PgANS,PgCHS and PgACT genes have been obtained,which has laid the foundation for further studying the influence of storage conditions on the anthocyanins of Punica granatum L.kernels.