Abstract: [Objective]The high-molecular-weight gluten subunits(HMW-GS)of [Method]88 wheat cultivars were analyzed by means of SDS-PAGE in order to make good use of crop germplasms resource of Xinjiang,[Result]a total of 15 HMW-GS pattern were identified.At Glu-A1 loci,3 alleles were detected,which encoded subunit 1,2~* and Null respectively and the major subunit was Null with the 57.96 .At Glu-B1 loci,8 alleles were detected,which encoded subunits 7+8,7+9 ,6+8,14+15,17+18,7,8 and20 ,7+8 and 7+9 were the major subunit patterns(45.46;,39.77;).4 alleles encoded subunits 5+10,2+12 ,3+12 and 2+11respectively were detected at Glu-D1 loci and 2+12 was the major subunit pattern(59.09;).There were 25 subunits combination patterns in wheat cultivars from Xinjiang wheat and the major pattern was"Null,7+8,2+12"with the frequency of 17.05.The frequency of high-quality HMW-GS(1,14+15,5+10) of strains was risen compared with early phase varieties,and the appearance rate of high-quality from other varielies (2~*,7+8,17+18) fell off to some extent. Among 88 wheat germplasm,32 carried 5+10 high-quality subunits,21 of that had 1 subunits,6 of that had 2~* subunits,1 of that had 14+15 subunits,1 of that had 17+18 subunits,and 2 of that had high quality subunits combinations(2~*,17+18,5+10,1,7+8,5+10),[Conclusion]these crop germplasms were utilized in wheat quality breeding.