随机质心映射优化法提取枸杞皮渣β-胡萝卜素及抗氧化活性分析

doi:10.6048/j.issn.1001-4330.2025.07.024

新疆农业科学 ›› 2025, Vol. 62 ›› Issue (7): 1794-1802.DOI: 10.6048/j.issn.1001-4330.2025.07.024

随机质心映射优化法提取枸杞皮渣β-胡萝卜素及抗氧化活性分析

梁丽鲜¹^,²(), 孟新涛¹^,³(), 刘一鸣¹^,², 张婷¹^,³, 马燕¹^,³, 许铭强¹^,³, 潘俨¹^,³()

1.新疆维吾尔自治区农业科学院农产品加工研究所, 乌鲁木齐 830091
2.新疆农业大学食品科学与药学学院, 乌鲁木齐 830091
3.新疆主要农副产品精深加工工程技术研究中心, 乌鲁木齐 830091

收稿日期:2024-12-07 出版日期:2025-07-20 发布日期:2025-09-05
通信作者: 孟新涛(1985-),女,副研究员,硕士,研究方向为农产品贮运与加工,(E-mail)mengxintao_929@126.com;
潘俨(1979-),男,研究员,博士,研究方向为农产品贮藏与加工,(E-mail)panyan1509@163.com
作者简介:梁丽鲜(2000-),女,硕士研究生,研究方向为农产品贮运与加工,(E-mail)2845887274@qq.com
基金资助:
新疆维吾尔自治区重点研发项目(2023B02009-1);新疆维吾尔自治区公益性科研院所基本科研业务费专项(KY2022013)

Extraction of β-carotene and antioxidant activity of Lycium barbarum dander by stochastic center-of-mass mapping optimization method

LIANG Lixian¹^,²(), MENG Xintao¹^,³(), LIU Yiming¹^,², ZHANG Ting¹^,³, MA Yan¹^,³, XU Mingqiang¹^,³, PAN Yan¹^,³()

1. Research Institute of Agro-product Storage and Processing, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China
2. College of Food Sciences and Pharmacy, Xinjiang Agricultural University, Urumqi 830091, China
3. Xinjiang Engineering and Technology Research Center for Intensive Processing of Major Agricultural By-products, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China

Received:2024-12-07 Published:2025-07-20 Online:2025-09-05
Supported by:
Key R & D Program Project of Xinjiang Uygur Autonomous Region(2023B02009-1);Basic Scientific R &D Program of Public Welfare Research Institutions of Xinjiang Uygur Autonomous Region(KY2022013)

摘要/Abstract

摘要：

【目的】研究优化新疆枸杞皮渣中β-胡萝卜素的较佳提取工艺,促进新疆枸杞资源的综合利用,为枸杞皮渣的高值化利用提供理论依据和技术支持。【方法】采用无水乙醇作为提取溶剂,考察料液比、温度、时间、提取次数对枸杞皮渣β-胡萝卜素提取量的影响。通过随机质心映射法(Random Centroid Optimization, RCO)优化提取工艺,确定较佳提取条件。并测定β-胡萝卜素对DPPH自由基和ABTS自由基的抗氧化能力。【结果】优化后的最佳提取工艺参数为料液比1∶16 g/mL、温度48℃、时间41 min、提取次数2次。在此条件下,β-胡萝卜素的提取量达到0.53 mg/g。β-胡萝卜素表现出良好的抗氧化能力,在质量浓度为1.0 mg/mL时,对DPPH和ABTS自由基的清除率分别为60.89%和66.11%。【结论】优化了枸杞皮渣中β-胡萝卜素的提取工艺。最佳提取条件为料液比1∶17 mg/mL、温度57℃、时间45 min、提取次数2次,提取量为0.53 μg/g。

关键词: 随机质心映射优化法(RCO); 枸杞皮渣; β-胡萝卜素提取; 抗氧化活性

Abstract:

【Objective】 The aim of this study is to optimize the better extraction process of β-carotene from the skin residue of Xinjiang Lycium barbarum in order to promote the comprehensive utilization of Xinjiang Lycium barbarum resources.Provides theoretical basis and technical support for the high-value utilization of Lycium barbarum dregs. 【Methods】 Anhydrous ethanol was used as the extraction solvent, and the effects of material-liquid ratio, temperature, time, and the number of extraction times on the extraction amount of β-carotene from the skin dregs of Lycium barbarum were investigated. The extraction process was optimized by Random Centroid Optimization (RCO), and then the better extraction conditions were determined. In addition, the antioxidant capacity of β-carotene against DPPH radicals and ABTS radicals was also determined. 【Results】 The optimized parameters of the best extraction process were: material-liquid ratio 1∶16 g/mL, temperature 48℃, time 41 min, and number of extractions twice. Under these conditions, the extracted amount of β-carotene reached 0.53 mg/g. β-carotene showed good antioxidant ability, and the scavenging rates of DPPH and ABTS radicals were 60.89% and 66.11% at a mass concentration of 1.0 mg/mL, respectively. 【Conclusion】

Key words: Stochastic center-of-mass mapping optimization (RCO) method; Lycium barbarum dander; β-carotene extraction; antioxidant activity

中图分类号:

S663.9

梁丽鲜, 孟新涛, 刘一鸣, 张婷, 马燕, 许铭强, 潘俨. 随机质心映射优化法提取枸杞皮渣β-胡萝卜素及抗氧化活性分析[J]. 新疆农业科学, 2025, 62(7): 1794-1802.

LIANG Lixian, MENG Xintao, LIU Yiming, ZHANG Ting, MA Yan, XU Mingqiang, PAN Yan. Extraction of β-carotene and antioxidant activity of Lycium barbarum dander by stochastic center-of-mass mapping optimization method[J]. Xinjiang Agricultural Sciences, 2025, 62(7): 1794-1802.

图/表 8

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循环次数 Cycles	待优化因子 Factor to be optimized	料液比 Material- liquid ratio (g/mL)	提取温度 Extraction temperature (℃)	提取时间 Extraction time(min)	提取次数 Extraction times
第一次随机质心搜索 The first random centroid search	因子上限	1∶5	25	20	1
第一次随机质心搜索 The first random centroid search	因子下限	1∶35	65	60	3
第一次随机质心搜索 The first random centroid search	因子上限	1∶17	55	38	1
第一次随机质心搜索 The first random centroid search	因子下限	1∶27	65	47	3

循环次数 Cycles	待优化因子 Factor to be optimized	料液比 Material- liquid ratio (g/mL)	提取温度 Extraction temperature (℃)	提取时间 Extraction time(min)	提取次数 Extraction times
第一次随机质心搜索 The first random centroid search	因子上限	1∶5	25	20	1
第一次随机质心搜索 The first random centroid search	因子下限	1∶35	65	60	3
第一次随机质心搜索 The first random centroid search	因子上限	1∶17	55	38	1
第一次随机质心搜索 The first random centroid search	因子下限	1∶27	65	47	3

循环次数 Number of cycles	试验编号 Experiment No.	料液比 Material- liquid ratio (g/mL)	提取温度 Extraction temperature (℃)	提取时间 Extraction time (min)	提取次数 Number of extractions	提取量 Extracted amount (mg /g)
第一轮循环随机搜索试验 First round of cyclic randomized search experiments	1	1∶15	44	51	2	0.22
	2	1∶23	32	21	1	0.46
	3	1∶22	58	51	2	0.39
	4	1∶31	60	41	2	0.23
	5	1∶34	36	22	2	0.12
	6	1∶5	57	39	2	0.29
	7	1∶30	61	38	3	0.18
	8	1∶15	32	60	1	0.14
	9	1∶24	40	45	2	0.11
第一次质心搜索 First center of gravity search	10	1∶20	52	38	2	0.43
	11	1∶16	48	41	2	0.52
	12	1∶23	49	41	2	0.46
	13	1∶19	48	38	2	0.24

循环次数 Number of cycles	试验编号 Experiment No.	料液比 Material- liquid ratio (g/mL)	提取温度 Extraction temperature (℃)	提取时间 Extraction time (min)	提取次数 Number of extractions	提取量 Extracted amount (mg /g)
第一轮循环随机搜索试验 First round of cyclic randomized search experiments	1	1∶15	44	51	2	0.22
	2	1∶23	32	21	1	0.46
	3	1∶22	58	51	2	0.39
	4	1∶31	60	41	2	0.23
	5	1∶34	36	22	2	0.12
	6	1∶5	57	39	2	0.29
	7	1∶30	61	38	3	0.18
	8	1∶15	32	60	1	0.14
	9	1∶24	40	45	2	0.11
第一次质心搜索 First center of gravity search	10	1∶20	52	38	2	0.43
	11	1∶16	48	41	2	0.52
	12	1∶23	49	41	2	0.46
	13	1∶19	48	38	2	0.24

随机质心映射优化法提取枸杞皮渣β-胡萝卜素及抗氧化活性分析

Extraction of β-carotene and antioxidant activity of Lycium barbarum dander by stochastic center-of-mass mapping optimization method

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参考文献 30

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Metrics

循环次数 Number of cycles	试验编号 Experiment No.	料液比 Material-liquid ratio(g/mL)	提取温度 Extraction temperature(℃)	提取时间 Extraction time(min)	提取次数 Number of extractions	提取量 Extracted amount(mg /g)
第二轮循环随机搜索试验 Second round of cyclic randomized search experiments	14	1∶24	63	39	3	0.23
	15	1∶21	57	46	1	0.43
	16	1∶21	56	42	2	0.41
	17	1∶18	64	43	2	0.32
	18	1∶26	60	46	2	0.21
	19	1∶21	61	42	1	0.35
	20	1∶25	61	43	2	0.44
第二次质心搜索 Second center of mass search	21	1∶20	57	46	2	0.41
	22	1∶16	57	43	2	0.28
	23	1∶17	57	44	2	0.51
	24	1∶17	57	45	2	0.53

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