新疆农业科学 ›› 2025, Vol. 62 ›› Issue (5): 1266-1272.DOI: 10.6048/j.issn.1001-4330.2025.05.025

• 草业·设施农业 • 上一篇    下一篇

新疆野生平菇的遗传多样性分析

罗影1,2(), 努尔孜亚·亚力买买提1(), 贾文捷1, 朱琦1, 石文婷2, 李雨桐2, 贾培松1()   

  1. 1.新疆维吾尔自治区农业科学院植物保护研究所/农业部西北荒漠绿洲作物有害生物综合治理重点实验室,乌鲁木齐 830091
    2.新疆农业大学,乌鲁木齐 830091
  • 收稿日期:2024-10-07 出版日期:2025-05-20 发布日期:2025-07-09
  • 通信作者: 努尔孜亚·亚力买买提(1977-),女,新疆人,副研究员,研究方向为食用菌,(E-mail) nrzy729@163.com;
    贾培松(1984-),男,河北人,副研究员,硕士,研究方向为食用菌食用菌,(E-mail) jps-fly@163.com
  • 作者简介:罗影(1988-),女,河北人,副研究员,研究方向为食用菌,(E-mail) sysly@xaas.ac.cn
  • 基金资助:
    天山英才培养计划;新疆维吾尔自治区创新环境(人才、基地)建设专项(PT2319);现代农业产业技术体系项目(CARS-20)

Identification and genetic diversity of wild Pleurotus in Xinjiang

LUO Ying1,2(), Nurziya Yarmamat1(), JIA Wenjie1, ZHU Qi1, LI Yutong2, SHI Wenting2, JIA Peisong1()   

  1. 1. Key Laboratory of Integrated Pest Management on Crop in Northwestern Oasis, Ministry of Agriculture and Rural Affairs / Institute of Plant Protection,Xinjiang Academy of Agricultural Sciences,Urumqi 830091, China
    2. Xinjiang Agricultural University, Urumqi 830091, China
  • Received:2024-10-07 Published:2025-05-20 Online:2025-07-09
  • Supported by:
    “Tianshan Talents” Cultivation Plan Project;Innovation Environment (Talent, Base) Construction Project(PT2319);China Agriculture Research System(CARS-20)

摘要:

【目的】分析新疆野生平菇的遗传多样性,为丰富和开发平菇新品种提供基础材料和理论依据。【方法】采用生物学和ITS、ISSR分子标记技术相结合的方法,研究40个新疆野生平菇菌株进行遗传多样性。【结果】40个新疆野生平菇菌株菌丝基本呈白色,菌落形态差异不显著,菌丝长势和长速方面有一定的区别。经ITS序列分析,可大致将40个菌株划分为4个类群;10条引物共扩增出83条清晰的DNA条带,多态性条带82条,多态性比率为98.80%。遗传相似系数为0.66时,可将40个样本分为5个类群,并显示出较强的地域相关性,供试的40株野生平菇菌株具有显著的遗传多样性。【结论】野生平菇菌株具有丰富的遗传多样性。

关键词: 平菇; 糙皮侧耳复合群; ITS; ISSR; 遗传多样性

Abstract:

【Objective】 The objective of the study is to analyze their genetic diversity of wild strains of the Pleurotus in Xinjiang in the hope of providing basic materials and theoretical basis for enriching and developing new varieties.【Methods】 The ITS sequence was used to identify the species and the ISSR markers technology to carry out the genetic diversity analysis on 40 wild oyster mushroom strains.【Results】 The mycelia of 40 strains of wild oyster mushroom in Xinjiang were basically white, and there were no significant differences in colony morphology, but certain differences in mycelia growth and growth speed. By ITS sequence analysis, 40 strains could be divided into 4 groups. 10 ISSR primers amplified a total of 83 clear DNA bands, including 82 polymorphic bands with a polymorphism ratio of 98.80%, and the 40 tested strains were divided into 5 groups at the genetic similarity coefficient of 0.66. In general, the 40 strains of wild oyster mushroom tested showed significant genetic diversity, which had important reference value for the breeding of Xinjiang oyster mushroom. 【Conclusion】 Pleurotus spp. strains have the occurrence of genetic differentiation with relatively rich genetic diversity.

Key words: oyster mushroom; Pleurotus ostreatus species complex; ITS; ISSR; genetic diversity

中图分类号: 


ISSN 1001-4330 CN 65-1097/S
邮发代号:58-18
国外代号:BM3342
主管:新疆农业科学院
主办:新疆农业科学院 新疆农业大学 新疆农学会

出版单位:《新疆农业科学》编辑部
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