新疆野生巴尔喀什蘑菇子实体多糖的提取与脱色工艺

doi:10.6048/j.issn.1001-4330.2024.06.027

新疆农业科学 ›› 2024, Vol. 61 ›› Issue (6): 1527-1534.DOI: 10.6048/j.issn.1001-4330.2024.06.027

新疆野生巴尔喀什蘑菇子实体多糖的提取与脱色工艺

努尔孜亚·亚力买买提¹(), 罗影¹, 贾培松¹, 郝敬喆¹(), 贾文捷¹(), 朱琦¹, 梁志豪², 蔺思茹², 赵振蒙²

1.新疆农业科学院植物保护研究所/农业部西北荒漠绿洲作物有害生物综合治理重点实验室,乌鲁木齐 830091
2.新疆农业大学农学院,乌鲁木齐 830091

收稿日期:2023-12-22 出版日期:2024-06-20 发布日期:2024-08-08
通信作者: 郝敬喆(1970-),男,江苏人,研究员,研究方向为微生物病理学,(E-mail)urmqhjz@qq.com;
贾文捷(1969-),男,河北人,高级农艺师,研究方向为食用菌栽培, (E-mail)1612367472@qq.com
作者简介:努尔孜亚·亚力买买提(1977-),女,新疆伊宁人,副研究员,研究方向为食用菌驯化与栽培, (E-mail)nrzy729@163.com
基金资助:
国家自然科学基金地区基金项目(32060709);国家自然科学基金地区基金项目(31560577);现代农业产业技术体系建设专项资金(CARS-20)

Extraction and decolorization of polysaccharides from fruit body of wild Agaricus balchaschensis in Xinjiang

Nuerziya Yalimaimaiti¹(), LUO Ying¹, JIA Peisong¹, HAO Jingzhe¹(), JIA Wenjie¹(), ZHU Qi¹, LIANG Zhihao², LIN Siru²

1. Key Laboratory of Integrated Crop Pest Management on Crop in Northwestern Oasis, Ministry of Agriculture and Rural Affairs/Institute of Plant Protection,Xinjiang Academy of Agricultural Sciences,Urumqi 830091,China
2. College of Agricalture Xinjiang Agriculture University, Urumqi 830052, China

Received:2023-12-22 Published:2024-06-20 Online:2024-08-08
Correspondence author: HAO Jingzhe (1970-), researcher, research direction: micropathology,( E-mail) urmqhjz@qq.com;
JIA Wenjie (1969-), senior agronomist, research direction: Edible fungi, (E-mail)1612367472@qq.com
Supported by:
National Natural Science Foundation Projects(32060709);National Natural Science Foundation Projects(31560577);Earmarked Fund for CARS(CARS-20)

摘要/Abstract

摘要：

【目的】研究新疆野生巴尔喀什蘑菇子实体粗多糖提取及多糖脱色工艺,为新疆野生巴尔喀什蘑菇多糖的纯化及深度开发提供理论依据和技术支持。【方法】釆用热水提醇沉法提取巴尔喀什蘑菇子实体粗多糖,研究提取温度、时间、次数对巴尔喀什蘑菇多糖得率的影响;选择脱色时间、脱色温度、活性炭和H₂O₂含量为影响因子,以多糖脱色率为研究指标,采用正交试验优化巴尔喀什蘑菇多糖脱色工艺,确定最佳脱色工艺条件。【结果】热水提醇沉法提取巴尔喀什菇子实体粗多糖的最佳工艺条件:提取时间6 h、提取温度60℃、提取次数2次,在此条件下巴尔喀什菇子实体粗多糖得率为15.6%。活性炭最佳脱色工艺为活性炭用量 25%、脱色温度 60℃、脱色时间45 min、pH值 3,在此条件下脱色率为80.4%;双氧水最佳脱色工艺为H₂O₂含量10%、脱色温度60℃、脱色时间3 h、pH值9,在此条件下脱色率为86.23%。【结论】利用热水提醇沉法提取巴尔喀什蘑菇粗多糖工艺具有实际应用价值。H₂O₂脱色法和活性炭脱色法对巴尔喀什蘑菇多糖的脱色效果受不同工艺条件的影响差异较大,H₂O₂脱色率比活性炭脱色率高,是巴尔喀什蘑菇多糖脱色的可行方法。

关键词: 巴尔喀什蘑菇; 多糖提取; 活性炭脱色; 双氧水(H₂O₂)脱色

Abstract:

【Objective】 To study the extraction and decolorization process of crude polysaccharides from fruit body of wild Agaricus balchaschensis in Xinjiang, and provide theoretical basis and technical support for its in-depth development. 【Methods】 This study adopted ethanol subsiding to extract the fruit body polysaccharides of wild A. balchaschensisin, and explored the influence of extraction temperature, time and frequency on the yield of polysaccharides from A. balchaschensisin. With the decolorization time, decolorization temperature, content of activated carbon and H₂O₂ as influencing factors and the decolorization rate of polysaccharides as research index, the study optimized the decolorization process of polysaccharides from A. balchaschensisin through orthogonal test, so as to determine the optimal decolorization conditions. 【Results】 According to the results, the optimal conditions for extracting fruit body polysaccharides of wild A. balchaschensis by ethanol subsiding were 6 h extraction time, 60℃ extraction temperature, and 2 times extraction, under which the yield of fruit body polysaccharides of wild A.balchaschensisin was 15.6%. The optimal decolorization process for activated carbon was 25% activated carbon, 60℃ decolorization temperature, and 45 min decolorization time at pH3, under which the decolorization rate was 80.4%. The optimal decolorization process for hydrogen peroxide was 10% H₂O₂ content, 60℃ decolorization temperature, and 3 h decolorization time at pH 9, under which the decolorization rate was 86.23%. 【Conclusion】 This technology has verified the feasibility and practical value of applying ethanol subsiding to extract fruit body polysaccharides of wild A.balchaschensis in and the effects of H₂O₂ and activated carbon decolorization methods on the decolorization of polysaccharides from A. balchaschensis varied greatly under different process conditions. The former has a higher decolorization rate than the latter and is feasible for the decolorization of polysaccharides from A.balchaschensisin.

Key words: Agaricus balchaschensis; carbon; hydrogen peroxide (H₂O₂ ) decolorization

中图分类号:

S646

努尔孜亚·亚力买买提, 罗影, 贾培松, 郝敬喆, 贾文捷, 朱琦, 梁志豪, 蔺思茹, 赵振蒙. 新疆野生巴尔喀什蘑菇子实体多糖的提取与脱色工艺[J]. 新疆农业科学, 2024, 61(6): 1527-1534.

Nuerziya Yalimaimaiti, LUO Ying, JIA Peisong, HAO Jingzhe, JIA Wenjie, ZHU Qi, LIANG Zhihao, LIN Siru. Extraction and decolorization of polysaccharides from fruit body of wild Agaricus balchaschensis in Xinjiang[J]. Xinjiang Agricultural Sciences, 2024, 61(6): 1527-1534.

图/表 10

表1 活性炭脱色正交试验水平因素

Tab.1 Activated carbon decolorization orthogonal experimenta level factor table

水平 Level	因素(Factors)
水平 Level	活性炭用量:A Activated carbon dosage (%)	时间:B Time (min)	温度: C Temperature (℃)	pH值:D pH value
1	25	35	50	2
2	30	45	60	3
3	35	55	70	4

表2 H2O2脱色正交试验水平因素

Tab.2 Hydrogen peroxide decolorization orthogonal experiment level factor table

水平 Level	因素Factors
水平 Level	温度(℃) Temperature A	时间(min) Time B	pH值 pH value C	H₂O₂ 浓度(%) H₂O₂ concentration D
1	50	1	9	8
2	60	2	10	10
3	70	3	11	12

图1 不同条件下多糖提取的变化

Fig.1 Changes of different conditions onpolysaccharide extraction

图2 葡萄糖标准曲线

Fig.2 Glucose standard curve

图3 不同活性炭用量下巴尔喀什蘑菇多糖脱色的变化

Fig.3 Changes of different amount of activated carbon on the decolorization of agaricus balchaschensis

图4 不同温度下巴尔喀什蘑菇多糖脱色的变化

Fig.4 Changes of different temperature on decolorization of agaricus balchaschensis

图5 不同时间下巴尔喀什蘑菇多糖脱色的变化

Fig.5 Changes of different time on the decolorization of agaricus balchaschensis

图6 不同pH值下巴尔喀什蘑菇多糖脱色的变化

Fig 6 Changes of different pH on decolorization of agaricus balchaschensis

表3 活性炭脱色正交试验

Tab.3 Decolorization of activated carbon Orthogonal experimental results

序号 Number	活性炭 Activated carbon (A)	时间 Time (B)	温度 Temperature (C)	pH值 pH value (D)	脱色率 Decolori zation rate(%)
1	1	1	1	1	54.16
2	1	2	2	2	80.40
3	1	3	3	3	53.40
4	2	1	2	3	70.65
5	2	2	3	1	48.21
6	2	3	1	2	66.31
7	3	1	3	2	51.97
8	3	2	1	3	74.19
9	3	3	2	1	50.71
K₁	62.65	58.93	64.89	51.03
K₂	61.72	67.60	67.25	66.23
K₃	58.95	58.81	51.19	66.08
R	3.70	8.79	16.06	15.20

表4 H2O2脱色正交试验

Tab.4 Hydrogen peroxide decolorization orthogonal experimental results

序号 Num- ber	温度 Temper- ature (A)	时间 Time (B)	pH值 pH value (C)	H₂O₂ (D)	脱色率 Decolorization rate(%)
1	1	1	1	1	63.83
2	1	2	2	2	78.05
3	1	3	3	3	79.5
4	2	1	2	3	70.36
5	2	2	3	1	81.09
6	2	3	1	2	86.23
7	3	1	3	2	63.32
8	3	2	1	3	76.89
9	3	3	2	1	76.59
K₁	73.93	65.84	75.65	74.84
K₂	79.23	78.69	75	75.87
K₃	72.27	81.11	74.64	75.58
R	6.96	15.27	1.01	1.03

参考文献 24

[1]	杨琴, 杜双田, 张桂香. 博湖蘑菇矿物质、脂肪酸成分分析[J]. 食品科学, 2013, 34(6): 231-233. DOI
	YANG Qin, DU Shuangtian, ZHANG Guixiang. Nutritional analysis of minerals and fatty acids in Bohu mushroom[J]. Food Science, 2013, 34(6): 231-233. DOI
[2]	迟桂荣, 徐琳, 吴继卫, 等. 杏鲍菇多糖的抗病毒、抗肿瘤研究[J]. 莱阳农学院学报, 2006, 23(3): 174-176.
	CHI Guirong, XU Lin, WU Jiwei, et al. Studies on anti-virus and anti-tumors exocellular polysaccharides of Pleurotus eryngii[J]. Journal of Laiyang Agricultural College, 2006, 23(3): 174-176.
[3]	杨琴, 杜双田, 郜小娟, 等. 博湖大蘑菇蛋白质营养价值评价[J]. 食品科学, 2009, 30(5): 100-103. DOI
	YANG Qin, DU Shuangtian, GAO Xiaojuan, et al. Nutritional assessment of Bohu mushroom protein[J]. Food Science, 2009, 30(5): 100-103. DOI
[4]	罗影, 刘醒, 关永强, 等. 新疆野生巴尔喀什蘑菇的化学成分分析[J]. 北方园艺, 2019, (22): 142-147.
	LUO Ying, LIU Xing, GUAN Yongqiang, et al. Analysis of chemical components to wildAgaricus balchaschensisin Xinjiang[J]. Northern Horticulture, 2019, (22): 142-147.
[5]	王如涛, 吴绵斌, 林建平, 等. 植物多糖分离提取技术的研究进展[J]. 中国生物工程杂志, 2013, 33(7): 118-123.
	WANG Rutao, WU Mianbin, LIN Jianping, et al. Recent advances in extraction and isolation of plant polysaccharides[J]. China Biotechnology, 2013, 33(7): 118-123.
[6]	阚建全, 王雅茜, 陈宗道, 等. 甘薯活性多糖抗突变作用的体外实验研究[J]. 中国粮油学报, 2001, 16(1): 23-27.
	KAN Jianquan, WANG Yaxi, CHEN Zongdao, et al. Antimutagenic activities of active polysaccharide extract from sweet potato based on Ames test[J]. Chinese Cereals and Oils Association, 2001, 16(1): 23-27.
[7]	商澎, 梅其炳, 曹之宪, 等. 当归多糖组分的高效液相色谱分析[J]. 中国药学杂志, 2000, 35(5): 332.
	SHANG Peng, MEI Qibing, Cao Zhixian, et al. Analysis ofAngelicapolysaccharides constituents by high-performance liquid chromatography[J]. Chinese Pharmaceutical Journal, 2000, 35(5): 332.
[8]	陈莉. 杏鲍菇多糖提取工艺优化及其产品研发[D]. 杭州: 浙江农林大学, 2013.
	CHEN Li. Pleurotus Eryngii Polysaccharide Extraction Process Optimization and the Product Research and Development[D]. Hangzhou: Zhejiang A & F University, 2013.
[9]	杨青松, 赵艳, 王莹, 等. 响应面法优化藏药红雪茶多糖提取工艺[J]. 食品工业, 2013, 34(8): 44-48.
	YANG Qingsong, ZHAO Yan, WANG Ying, et al. Optimization extraction conditions of polysaccharide from Tibetan medicine Lethariella spp. by response surface method[J]. The Food Industry, 2013, 34(8): 44-48.
[10]	陶文沂, 敖宗华, 许泓瑜, 等. 药食用真菌生物技术[M]. 北京: 化学工业出版社, 2007.
	TAO Wenyi, AO Zonghua, XU Hongyu, et al. Biotechnology of medicinal and edible fungi[M]. Beijing: Chemical Industry Press, 2007.
[11]	蒋俊, 杨焱, 罗玺, 等. 猴头菌多糖不同脱色方法的研究[J]. 天然产物研究与开发, 2013, 25(9): 1180-1184.
	JIANG Jun, YANG Yan, LUO Xi, et al. Study on different decolorization methods ofHericium erinaceuspolysaccharide[J]. Natural Product Research and Development, 2013, 25(9): 1180-1184.
[12]	谢建华, 申明月, 聂少平, 等. 青钱柳多糖活性炭脱色工艺[J]. 南昌大学学报, 2013, 37(4):382-385.
	XIE Jianhua, SHI Shiyue, NIE Shaoping, et al. Decolorization process of Cyclocarya paliurus polysaccharide activated carbon[J]. Journal of Nanchang University, 2013, 37 (4): 382-385.
[13]	王文彤, 张岩, 陶遵威. 正交试验优化苦豆子多糖的双氧水脱色工艺研究[J]. 现代药物与临床, 2014, 29(11): 1234-1237.
	WANG Wentong, ZHANG Yan, TAO Zunwei. Optimization of decolorization technology of Sophora alopecuroides polysaccharide with H2O2 by orthogonal test[J]. Drugs & Clinic, 2014, 29(11): 1234-1237.
[14]	宫春宇, 余世锋, 郑喜群. 响应面法优化玉米须多糖氧化脱色工艺[J]. 粮食与油脂, 2015, 28(8):31-34.
	GONG Chunyu, YU Shifeng, ZHENG Xiqun. Optimization of oxidative decolorization process of corn silk polysaccharide by response surface methodology[J]. Cereals and Oil, 2015, 28 (8): 31-34.
[15]	肖杰, 姚凌云, 孙健, 等. 树脂法同时脱除虫草粗多糖中色素与蛋白质[J]. 食品工业科技, 2012, 33(7): 236-239, 243.
	XIAO Jie, YAO Lingyun, SUN Jian, et al. Simultaneous removal of pigment and protein in crude Cordyceps polysaccharide by resin[J]. Science and Technology of Food Industry, 2012, 33(7): 236-239, 243.
[16]	国家药典委员会. 中华人民共和国药典[M]. 北京: 中国医药科技出版社, 2015: 283.
	National Pharmacopoeia Commission. Pharmacopoeia of the People's Republic of China[M]. Beijing: China Medical Science Press, 2015: 283.
[17]	朱静, 贾毓宁. 白牛杆菌多糖脱色工艺的优化及其抗氧化活性[J]. 中成药, 2018, 40( 4) : 825-830.
	ZHU Jing, JIA Yuning. Optimization of decolorization process and antioxidant activity of bovine polysaccharide[J]. Chinese Traditional Patent Medicine, 2018, 40(4): 825 - 830.
[18]	张华林, 王冬梅, 杨红艳, 等. 树脂吸附对巴戟天多糖脱色脱蛋白的工艺研究[J]. 现代食品科技, 2012, 28(12):1709-1713.
	ZHANG Hualin, WANG Dongmei, YANG Hongyan, et al. Study on the process of decolorization and deproteinization of Morinda officinalis polysaccharides by resin adsorption[J]. Modern Food Science and Technology, 2012, 28 (12): 1709-1713.
[19]	张军霞, 胡喜巧. 香菇多糖几种提取方法的比较[J]. 河南科技学院学报, 2008, 36(1):45-49.
	ZHANG Junxia, HU Xiqiao, et al. The comparative study of several extraction methods of mushrooms polysaccharide[J]. Journal of Henan Institute of Science and Technology, 2008, 36(1):45-49.
[20]	陶文沂, 敖宗华, 许泓瑜, 等. 药食用真菌生物技术[M]. 北京: 化学工业出版社, 2007.
	TAO Wenyi, AO Zonghua, XU Hongyu, et al. Biotechnology of medicinal and edible fungi[M]. Beijing: Chemical Industry Press, 2007.
[21]	常昕. 博湖蘑菇人工栽培关键因素及多糖提取工艺研究[D]. 西北农林科技大学, 2011.
	CHANG Xin. Study on the key factors of artificial cultivation and polysaccharide extraction technology of Bohu mushroom[D]. Yangling: Northwest A&F University, 2011.
[22]	Gao Y H, Tang W B, Gao H, et al. Ganoderma lucidum polysaccharide fractions accelerate healing of acetic acid-induced ulcers in rats[J]. Journal of Medicinal Food, 2004, 7(4): 417-421.
[23]	王维香, 王晓君, 黄潇, 等. 川芎多糖脱色方法比较[J]. 离子交换与吸附, 2010, 26(1): 74-82.
	WANG Weixiang, WANG Xiaojun, HUANG Xiao, et al. Comparison study of decolorization ways for polyssacharide from Ligusticum chuanxiong hort[J]. Ion Exchange and Adsorption, 2010, 26(1): 74-82.
[24]	陈健, 耿安静, 徐晓飞. 香菇多糖的过氧化氢脱色工艺研究[J]. 食品工业科技, 2010, 31(3): 293-295.
	CHEN Jian, GENG Anjing, XU Xiaofei. Study on decolorizing technology of Ientinan by hydrogen peroxide[J]. Science and Technology of Food Industry, 2010, 31(3): 293-295.

编辑推荐 0

Metrics

阅读次数

全文

HTML			PDF

最新录用	在线预览	正式出版	最新录用	在线预览	正式出版
0	0	4	0	0	18

来源	本网站	其他网站

次数	16	6
比例	73%	27%

摘要

101

最新录用	在线预览	正式出版

0	0	101

来源	本网站	其他网站

次数	29	72
比例	29%	71%

新疆野生巴尔喀什蘑菇子实体多糖的提取与脱色工艺

Extraction and decolorization of polysaccharides from fruit body of wild Agaricus balchaschensis in Xinjiang

在线阅读

PDF下载

可视化

摘要/Abstract

引用本文

使用本文

图/表 10

参考文献 24

相关文章 3

编辑推荐 0

Metrics

[1]	努尔孜亚·亚力买买提, 郝敬喆, 贾文捷, 陈浩宇, 罗影, 贾培松. 巴尔喀什蘑菇子实体漆酶纯化与理化特性及对酚类化合物的降解能力[J]. 新疆农业科学, 2023, 60(2): 432-439.
[2]	罗影, 关永强, 祁正祥, 郝敬喆, 努尔孜亚·亚力买买提, 魏鹏, 贾文捷, 贾培松. 外源营养因子对巴尔喀什蘑菇菌丝的影响[J]. 新疆农业科学, 2021, 58(1): 133-142.
[3]	努尔孜亚·亚力买买提, 魏鹏, 贾培松, 罗影, 郝敬喆, 贾文捷. 一种巴尔喀什蘑菇发酵料腐生菌的鉴定及其生物学特性研究[J]. 新疆农业科学, 2017, 54(8): 1489-1495.