PRRSV HNHK3-2021毒株的ORF5和ORF7序列分析

doi:10.6048/j.issn.1001-4330.2024.05.028

新疆农业科学 ›› 2024, Vol. 61 ›› Issue (5): 1292-1300.DOI: 10.6048/j.issn.1001-4330.2024.05.028

• 草业·畜牧兽医 • 上一篇

PRRSV HNHK3-2021毒株的ORF5和ORF7序列分析

范悦轩¹^,²(), 马佳镁¹^,², 黄春媛², 郑佳馨², 陈素贞¹^,², 张艳², 刘光亮¹^,³(), 曹宗喜¹^,²()

1.新疆农业大学动物医学学院,乌鲁木齐 830000
2.海南省农业科学院畜牧兽医研究所/海南省热带动物繁育与疫病研究重点实验室,海口 571100
3.海南省农业科学院畜牧兽医研究所海南省院士团队创新中心,海口 571100

收稿日期:2023-10-12 出版日期:2024-05-20 发布日期:2024-07-09
通信作者: 曹宗喜(1982-),男,河南新乡人,研究员,博士,硕士生/博士生导师,研究方向为家畜传染病学,(E-mial)caozongxi@163.com;
刘光亮(1975-),男,四川资中人,研究员,博士,硕士生/博士生导师,研究方向为动物疫苗研制及分子免疫学,(E-mial)liuguangliang01@caas.cn
作者简介:范悦轩(1997-),男,新疆奇台人,硕士研究生,研究方向为家畜传染病学,(E-mial)1245219231@qq.com
基金资助:
海南省省属科研院所技术创新专项(jscx202001);国家自然科学基金项目(32060796);家畜疫病病原生物学国家重点实验室开放基金(SKLVEB2020KFKT021);海南省农业科学院院本级科研项目(HAAS2022PT0205);国家重点研发计划(2023YFC3404302)

Sequence analysis of ORF3 and ORF5 of PRRSV HNHK3-2021 strain isolated in Hainan

FAN Yuexuan¹^,²(), MA Jiamei¹^,², HUANG Chunyuan², ZHENG Jiaxin², CHEN Suzhen¹^,², ZHANG Yan², LIU Guangliang¹^,³(), CAO Zongxi¹^,²()

1. College of Veterinary Medicine, Xinjiang Agricultural University, Urumqi 830052, China
2. Hainan Provincial Key Laboratory of Tropical Animal Breeding and Disease Research, Institute of Animal Husbandry and Veterinary Medicine, Hainan Academy of Agricultural Sciences, Haikou 571100, China
3. Hainan Provincial Academician Innovation Center, Institute of Animal Husbandry and Veterinary Medicine, Hainan Academy of Agricultural Sciences, Haikou 571100, China

Received:2023-10-12 Published:2024-05-20 Online:2024-07-09
Correspondence author: CAO Zongxi (1982- ), male, from Xinxiang Henan, researcher, doctor, doctoral supervisor, research direction: livestock infectious diseases, (E-mail)caozongxi@163.com;
LIU Guangliang (1975- ), male, from Zizhong Sichuan, researcher, doctor, doctoral supervisor, research direction:animal vaccine development and molecular immunology, (E-mail)liuguangliang01@caas.cn
Supported by:
Technological Innovation Project of Hainan Provincial Scientific Research Institutes(jscx202001);The National Natural Science Foundation of China(32060796);Open Fund of the State Key Laboratory of Pathogenic Biology of Livestock Diseases(SKLVEB2020KFKT021);Hainan Provincial Academy of Agricultural Sciences Research Project at the same level(HAAS2022PT0205);National Key R&D Program of China(2023YFC3404302)

摘要/Abstract

摘要：

【目的】研究海南猪繁殖与呼吸综合征病毒PRRSV的流行病学特征和遗传变异规律。【方法】采集海南省海口市及周边地区样品,分离出PRRSV HNHK3-2021 毒株,并测定NSP2基因以及 ORF5 和ORF7基因序列,采用Lasergene 7.1进行序列比对分析,用MEGA-X进行遗传演化分析。【结果】分离出的PRRSV HNHK3-2021株为美洲型毒株,PRRSV HNHK3-2021株和EF112447 HEB1、EU109503 GD、EU624117 XH-GD的ORF5和ORF7的核苷酸相似性较高,分别为99.2%~99.3%和99.5%~100%,推导氨基酸相似性为99%~99.5%和100%;PRRSV HNHK3-2021株和EU864232 SHB、AY262352_HB-2(sh)2002、AY656990 Abst-1、AY032626 CH-1a、EU807840 CH-1R的ORF5和ORF7的核苷酸相似性为87.1%~96.4%和91.4%~97.3%,推导氨基酸的相似性为86.6%~93%和91.9%~97.6%;PRRSV HNHK3-2021株和JN654459 NADC30、KP860909 FJZ03、MH068878 SD17-38、AY881994 FJ-1的ORF5和ORF7的核苷酸相似性为85.4%~87.1%和89%~91.4%。氨基酸的相似性为86.1%~87.1%和89.5%~91.9%。【结论】RRSV HNHK3-2021 毒株和EU624117 XH-GD具有最近的遗传距离。

关键词: 猪繁殖与呼吸综合征; 遗传进化; ORF5; ORF7

Abstract:

【Objective】To explore the epidemiological characteristics and genetic variation of PRRSV in Hainan【Methods】Samples were collected from Haikou and surrounding areas.The PRRSV strain HNHK3-2021 was isolated, and the NSP2 genes, as well as the ORF5 and ORF7 gene sequences, were determined.Sequence alignment analysis was conducted using Lasergene 7.1, and genetic evolution analysis was performed using MEGA-X.【Results】The PRRSV HNHK3-2021 strain isolated was found to be of American origin with high nucleotide similarity to EF112447 HEB1, EU109503 GD, and EU624117 XH-GD ORF5 and ORF7 (99.2%~99.3% and 99.5%~100%, respectively).The derived amino acid similarity was 99%~99.5% and 100%.The nucleotide similarities of ORF5 and ORF7 of PRRSV HNHK3-2021 strain and EU864232 SHB, AY262352_HB-2(sh)2002, AY656990 Abst-1, AY032626 CH-1a, EU807840 CH-1R were found to be 87.1% -96.4% and 91.4%-97.3%, respectively.Similarly, the similarity of amino acids was 86.6%-93% and 91.9%-97.6%.The nucleotide similarities of ORF5 and ORF7 of PRRSV HNHK3-2021 strain and JN654459 NADC30, KP860909 FJZ03, MH068878 SD17-38, AY881994 FJ-1 were found to be 85.4%-87.1% and 89%-91.4%.The similarity of amino acids was 86.1%-87.1% and 89.5%-91.9%.【Conclusion】The PRRSV HNHK3-2021 strain and EU624117 XH-GD have the closest genetic distance.

Key words: porcine reproductive and respiratory syndrome; genetic evolution analysis; ORF5; ORF7

中图分类号:

范悦轩, 马佳镁, 黄春媛, 郑佳馨, 陈素贞, 张艳, 刘光亮, 曹宗喜. PRRSV HNHK3-2021毒株的ORF5和ORF7序列分析[J]. 新疆农业科学, 2024, 61(5): 1292-1300.

FAN Yuexuan, MA Jiamei, HUANG Chunyuan, ZHENG Jiaxin, CHEN Suzhen, ZHANG Yan, LIU Guangliang, CAO Zongxi. Sequence analysis of ORF3 and ORF5 of PRRSV HNHK3-2021 strain isolated in Hainan[J]. Xinjiang Agricultural Sciences, 2024, 61(5): 1292-1300.

图/表 7

参考文献 20

[1]	Wensvoort G, Terpstra C, Pol J M, et al. Mystery swine disease in The Netherlands: the isolation of Lelystad virus[J]. The Veterinary Quarterly, 1991, 13(3): 121-130.
[2]	周峰, 郭龙飞, 刘红英, 等. 猪繁殖与呼吸综合征病毒XINX株的分离及NSP2基因的遗传进化分析[J]. 江苏农业科学, 2014, 42(9): 180-182.
	ZHOU Feng, GUO Longfei, LIU Hongying, et al. Isolation of porcine reproductive and respiratory syndrome virus XINX strain and genetic evolution analysis of NSP2 gene[J]. Jiangsu Agricultural Sciences, 2014, 42(9): 180-182.
[3]	王亚磊, 茆达干, 孟春花, 等. PRRSV非结构蛋白与病毒逃逸宿主免疫反应的机制[J]. 江苏农业科学, 2014, 42(5): 165-167.
	WANG Yalei, MAO Dagan, MENG Chunhua, et al. Mechanism of non-structural protein of PRRSV and virus escaping from host immune response[J]. Jiangsu Agricultural Sciences, 2014, 42(5): 165-167.
[4]	Allaire M, Chernaia M M, Malcolm B A, et al. Picornaviral 3C cysteine proteinases have a fold similar to chymotrypsin-like serine proteinases[J]. Nature, 1994, 369(6475): 72-76.
[5]	Zhou S C, Ge X N, Kong C, et al. Characterizing the PRRSV nsp2 deubiquitinase reveals dispensability of Cis-activity for replication and a link of nsp2 to inflammation induction[J]. Viruses, 2019, 11(10): 896.
[6]	Weiland E, Wieczorek-Krohmer M, Kohl D, et al. Monoclonal antibodies to the GP5 of porcine reproductive and respiratory syndrome virus are more effective in virus neutralization than monoclonal antibodies to the GP4[J]. Veterinary Microbiology, 1999, 66(3): 171-186. PMID
[7]	Guzmán M, Meléndez R, Jiménez C, et al. Analysis of ORF5 sequences of Porcine Reproductive and Respiratory Syndrome virus (PRRSV) circulating within swine farms in Costa Rica[J]. BMC Veterinary Research, 2021, 17(1): 217. DOI PMID
[8]	Olasz F, Dénes B, BálintÁ, et al. Immunological and biochemical characterisation of 7ap, a short protein translated from an alternative frame of ORF7 of PRRSV[J]. Acta Veterinaria Hungarica, 2016, 64(2): 273-287. DOI PMID
[9]	BálintÁ, Molnár T, Kecskeméti S, et al. Genetic variability of PRRSV vaccine strains used in the national eradication programme, Hungary[J]. Vaccines, 2021, 9(8): 849.
[10]	贺冬梅, 谭树义, 陈朝喜, 等. PRRSV HuN-2011株ORF5和ORF7基因的序列分析[J]. 黑龙江畜牧兽医, 2017(7): 188-191.
	HE Dongmei, TAN Shuyi, CHEN Zhao Xi, et al. Sequence analysis of ORF5 and ORF7 genes of PRRSV HuN-2011 strain[J]. Heilongjiang Animal Science and Veterinary Medicine, 2017(7): 188-191.
[11]	秦宏阳, 曹宗喜, 汤国凯, 等. 4株PRRSV GP5蛋白编码序列的序列测定和特性分析[J]. 广东畜牧兽医科技, 2008, 33(5): 25-28.
	QIN Hongyang, CAO Zongxi, TANG Guokai, et al. Sequencing and analysis of four PRRSV strains' GP5 proteins[J]. Guangdong Journal of Animal and Veterinary Science, 2008, 33(5): 25-28.
[12]	Cao Z X, Jiao P R, Huang Y M, et al. Genetic diversity analysis of the ORF5 gene in porcine reproductive and respiratory syndrome virus samples from South China[J]. Acta Veterinaria Hungarica, 2012, 60(1): 157-164.
[13]	曹宗喜, 潘全会, 林哲敏, 等. PRRSV广东株XH-GD的分离及其NSP2、ORF3、ORF5的序列分析[J]. 广东农业科学, 2013, 40(12): 159-163.
	CAO Zongxi, PAN Quanhui, LIN Zhemin, et al. Isolation and sequence analysis of NSP2, ORF3 and ORF5 of PRRSV XH-GD strain from Guangdong Province[J]. Guangdong Agricultural Sciences, 2013, 40(12): 159-163.
[14]	Guo Z H, Chen X X, Li R, et al. The prevalent status and genetic diversity of porcine reproductive and respiratory syndrome virus in China: a molecular epidemiological perspective[J]. Virology Journal, 2018, 15(1): 2. DOI PMID
[15]	郭宝清, 陈章水. 哈兽研所首次证实国内猪群存在PRRSV感染[J]. 兽医科技信息, 1996,(1): 8.
	GUO Baoqing, CHEN Zhangshui. Harbin Institute of Veterinary Medicine confirmed for the first time that PRRSV infection exists in domestic pigs[J]. Chinese Journal of Animal Husbandry and Veterinary Medicine, 1996,(1): 8.
[16]	吴鹏, 张勋, 梁田, 等. 北疆地区猪繁殖与呼吸综合征病毒ORF7基因克隆与遗传演化分析[J]. 新疆农业科学, 2016, 53(11): 2149-2156.
	WU Peng, ZHANG Xun, LIANG Tian, et al. PRRSV ORF7 cloning and genetic evolution analysis of pigs in northern Xinjiang[J]. Xinjiang Agricultural Sciences, 2016, 53(11): 2149-2156.
[17]	An T Q, Zhou Y J, Liu G Q, et al. Genetic diversity and phylogenetic analysis of glycoprotein 5 of PRRSV isolates in mainland China from 1996 to 2006: coexistence of two NA-subgenotypes with great diversity[J]. Veterinary Microbiology, 2007, 123(1/2/3): 43-52.
[18]	陆桂丽, 汪萍, 成进, 等. 猪繁殖与呼吸综合征新疆株ORF2-7基因的克隆及序列分析[J]. 新疆农业科学, 2014, 51(3): 546-551.
	LU Guili, WANG Ping, CHENG Jin, et al. Clone and sequence analysis of ORF2-7 genes of porcine reproductive and respiratory syndrome virus of Xinjiang strains[J]. Xinjiang Agricultural Sciences, 2014, 51(3): 546-551.
[19]	Suárez P, Zardoya R, Martín M J, et al. Phylogenetic relationships of European strains of porcine reproductive and respiratory syndrome virus (PRRSV) inferred from DNA sequences of putative ORF-5 and ORF-7 genes[J]. Virus Research, 1996, 42(1/2): 159-165.
[20]	王旭哲, 刘永宏, 赵丽, 等. 新疆南疆猪繁殖与呼吸综合征病毒ORF5基因克隆与序列分析[J]. 新疆农业科学, 2013, 50(2): 357-364.
	WANG Xuzhe, LIU Yonghong, ZHAO Li, et al. The Cloning and the Sequence Analysis of Southern Xinjiang Strain ORF5 Gene of Porcine Reproductive and Respiratory Syndrome Virus[J]. Xinjiang Agricultural Sciences, 2013, 50(2): 357-364.

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项目 Items	引物名称 Name of primer	引物序列 Primer sequences	扩增长度 Amplification length
NSP2	NSP2-F	5'-TCGGTGCACGTGTACCC-3'	1 968
NSP2	NSP3-R	5'-GGCGGGGAAACTCTCTC-3'	1 968
ORF5	ORF5-F	5'-ATGTTGGGGAAGTGCTTGACCGC-3'	602
ORF5	ORF5-R	5'-CTAGAGACGACCCCATTGTTC-3'	602
ORF7	ORF7-F	5'-GAATTCATGCCAAATAACAACGGC-3'	372
ORF7	ORF7-R	5'-TCTAGATCATGTTGGGGGTGATGC-3'	372

项目 Items	引物名称 Name of primer	引物序列 Primer sequences	扩增长度 Amplification length
NSP2	NSP2-F	5'-TCGGTGCACGTGTACCC-3'	1 968
NSP2	NSP3-R	5'-GGCGGGGAAACTCTCTC-3'	1 968
ORF5	ORF5-F	5'-ATGTTGGGGAAGTGCTTGACCGC-3'	602
ORF5	ORF5-R	5'-CTAGAGACGACCCCATTGTTC-3'	602
ORF7	ORF7-F	5'-GAATTCATGCCAAATAACAACGGC-3'	372
ORF7	ORF7-R	5'-TCTAGATCATGTTGGGGGTGATGC-3'	372

	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21
1		99.2	99.2	99.1	98.7	85.2	99.2	99.6	99.0	95.5	95.8	92.6	93.5	92.6	86.0	86.0	86.3	86.4	85.9	86.5	87.0
2	98.4		100.0	99.9	99.2	85.7	100.0	99.6	99.7	96.2	96.6	93.4	94.3	93.1	86.5	89.4	86.8	86.9	86.2	86.8	87.5
3	98.4	100.0		99.9	99.2	85.7	100.0	99.6	99.7	96.2	96.6	93.4	94.3	93.1	86.5	89.4	86.8	86.9	86.2	86.8	87.5
4	98.4	100.0	100.0		99.1	85.6	99.9	99.5	99.6	96.1	96.5	93.3	94.2	93.0	86.4	89.2	86.6	86.8	86.3	86.6	87.4
5	98.4	100.0	100.0	100.0		85.0	99.2	99.1	99.0	95.8	96.2	92.9	93.8	92.6	85.7	89.4	86.3	86.4	85.7	86.0	86.8
6	84.0	85.2	85.2	85.2	85.2		85.7	85.6	85.7	85.7	86.0	86.8	85.1	86.3	95.6	85.0	95.2	94.6	94.7	94.2	94.2
7	98.4	100.0	100.0	100.0	100.0	85.2		99.6	99.7	96.2	96.6	93.4	94.3	96.1	86.5	89.4	86.8	86.9	86.2	86.8	87.5
8	98.8	99.6	99.6	99.6	99.6	84.8	99.6		99.4	95.8	96.2	93.0	93.9	93.0	86.4	89.5	86.6	86.8	86.3	86.6	87.4
9	97.7	99.2	99.2	99.2	99.2	85.2	99.2	98.8		96.0	96.4	93.1	94.0	92.9	86.5	89.4	86.8	86.9	86.2	87.0	87.5
10	94.6	95.7	95.7	95.7	95.7	85.6	95.7	95.3	94.9		99.1	94.8	95.6	95.1	87.3	90.0	86.9	87.7	86.5	87.5	88.1
11	94.9	96.5	96.5	96.5	96.5	86.0	96.5	96.1	95.7	98.4		95.1	96.2	94.8	87.5	90.3	87.2	87.9	86.7	87.3	88.1
12	91.8	93.4	93.4	93.4	93.4	86.4	93.4	93.0	92.6	93.0	94.2		92.6	95.5	87.7	90.1	87.3	87.0	87.4	87.7	87.4
13	92.2	93.8	93.8	93.8	93.8	82.9	93.8	93.4	93.0	94.6	95.7	90.7		92.6	86.5	88.6	86.5	87.2	85.5	86.0	86.9
14	92.6	93.0	93.0	93.0	93.0	84.0	93.0	93.4	92.2	94.9	94.6	93.0	92.2		87.3	89.9	86.9	86.9	87.1	87.5	87.0
15	85.2	86.4	86.4	86.4	86.4	94.2	86.4	86.0	86.4	88.3	88.7	87.9	86.4	86.4		85.7	96.5	95.6	95.7	95.6	96.0
16	86.8	87.5	87.5	87.5	87.5	83.7	87.5	87.9	87.5	88.3	88.7	89.5	86.8	89.1	86.0		85.3	85.5	85.8	85.6	86.3
17	86.4	87.5	87.5	87.5	87.5	94.2	87.5	87.2	87.5	88.3	88.7	88.7	85.6	87.5	96.1	85.6		94.4	95.2	95.2	95.7
18	86.4	87.5	87.5	87.5	87.5	93.0	87.5	87.2	87.5	89.1	89.5	87.9	86.4	87.2	95.3	86.8	93.4		95.1	94.6	94.6
19	85.9	86.3	86.3	86.3	86.3	93.4	86.3	86.7	86.3	88.3	88.7	88.7	85.5	88.3	96.1	87.9	95.7	94.1		95.1	94.4
20	87.9	88.3	88.3	88.3	88.3	92.6	88.3	87.9	89.1	89.1	88.7	88.3	86.0	87.5	94.6	86.4	95.3	94.2	94.9		94.8
21	85.6	86.8	86.8	86.8	86.8	91.8	86.8	86.4	86.8	88.3	87.9	87.2	84.8	86.4	95.3	87.2	94.6	93.4	94.5	94.6

	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21
1		99.2	99.2	99.1	98.7	85.2	99.2	99.6	99.0	95.5	95.8	92.6	93.5	92.6	86.0	86.0	86.3	86.4	85.9	86.5	87.0
2	98.4		100.0	99.9	99.2	85.7	100.0	99.6	99.7	96.2	96.6	93.4	94.3	93.1	86.5	89.4	86.8	86.9	86.2	86.8	87.5
3	98.4	100.0		99.9	99.2	85.7	100.0	99.6	99.7	96.2	96.6	93.4	94.3	93.1	86.5	89.4	86.8	86.9	86.2	86.8	87.5
4	98.4	100.0	100.0		99.1	85.6	99.9	99.5	99.6	96.1	96.5	93.3	94.2	93.0	86.4	89.2	86.6	86.8	86.3	86.6	87.4
5	98.4	100.0	100.0	100.0		85.0	99.2	99.1	99.0	95.8	96.2	92.9	93.8	92.6	85.7	89.4	86.3	86.4	85.7	86.0	86.8
6	84.0	85.2	85.2	85.2	85.2		85.7	85.6	85.7	85.7	86.0	86.8	85.1	86.3	95.6	85.0	95.2	94.6	94.7	94.2	94.2
7	98.4	100.0	100.0	100.0	100.0	85.2		99.6	99.7	96.2	96.6	93.4	94.3	96.1	86.5	89.4	86.8	86.9	86.2	86.8	87.5
8	98.8	99.6	99.6	99.6	99.6	84.8	99.6		99.4	95.8	96.2	93.0	93.9	93.0	86.4	89.5	86.6	86.8	86.3	86.6	87.4
9	97.7	99.2	99.2	99.2	99.2	85.2	99.2	98.8		96.0	96.4	93.1	94.0	92.9	86.5	89.4	86.8	86.9	86.2	87.0	87.5
10	94.6	95.7	95.7	95.7	95.7	85.6	95.7	95.3	94.9		99.1	94.8	95.6	95.1	87.3	90.0	86.9	87.7	86.5	87.5	88.1
11	94.9	96.5	96.5	96.5	96.5	86.0	96.5	96.1	95.7	98.4		95.1	96.2	94.8	87.5	90.3	87.2	87.9	86.7	87.3	88.1
12	91.8	93.4	93.4	93.4	93.4	86.4	93.4	93.0	92.6	93.0	94.2		92.6	95.5	87.7	90.1	87.3	87.0	87.4	87.7	87.4
13	92.2	93.8	93.8	93.8	93.8	82.9	93.8	93.4	93.0	94.6	95.7	90.7		92.6	86.5	88.6	86.5	87.2	85.5	86.0	86.9
14	92.6	93.0	93.0	93.0	93.0	84.0	93.0	93.4	92.2	94.9	94.6	93.0	92.2		87.3	89.9	86.9	86.9	87.1	87.5	87.0
15	85.2	86.4	86.4	86.4	86.4	94.2	86.4	86.0	86.4	88.3	88.7	87.9	86.4	86.4		85.7	96.5	95.6	95.7	95.6	96.0
16	86.8	87.5	87.5	87.5	87.5	83.7	87.5	87.9	87.5	88.3	88.7	89.5	86.8	89.1	86.0		85.3	85.5	85.8	85.6	86.3
17	86.4	87.5	87.5	87.5	87.5	94.2	87.5	87.2	87.5	88.3	88.7	88.7	85.6	87.5	96.1	85.6		94.4	95.2	95.2	95.7
18	86.4	87.5	87.5	87.5	87.5	93.0	87.5	87.2	87.5	89.1	89.5	87.9	86.4	87.2	95.3	86.8	93.4		95.1	94.6	94.6
19	85.9	86.3	86.3	86.3	86.3	93.4	86.3	86.7	86.3	88.3	88.7	88.7	85.5	88.3	96.1	87.9	95.7	94.1		95.1	94.4
20	87.9	88.3	88.3	88.3	88.3	92.6	88.3	87.9	89.1	89.1	88.7	88.3	86.0	87.5	94.6	86.4	95.3	94.2	94.9		94.8
21	85.6	86.8	86.8	86.8	86.8	91.8	86.8	86.4	86.8	88.3	87.9	87.2	84.8	86.4	95.3	87.2	94.6	93.4	94.5	94.6

	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21
1		99.5	99.5	99.5	99.5	99.5	100.0	98.9	99.7	93.3	96.0	96.0	93.5	93.3	89.2	90.9	88.4	88.4	99.5	90.6	91.9
2	99.5		99.5	99.5	99.5	99.5	99.5	98.9	99.7	93.3	96.0	96.0	93.5	93.3	89.2	90.9	88.4	87.9	99.5	90.6	91.9
3	99.5	99.5		99.5	99.5	99.5	99.5	98.9	99.7	93.3	96.0	96.0	93.5	93.3	89.2	90.9	88.4	87.9	99.5	90.6	91.9
4	99.5	99.5	99.5		99.5	99.5	99.5	98.9	99.7	93.3	96.0	96.0	93.5	93.3	89.8	91.4	89.0	88.4	99.5	91.1	92.5
5	99.5	99.5	99.5	99.5		99.5	99.5	98.9	99.7	93.3	96.0	96.0	93.5	93.3	89.2	90.9	88.4	88.2	100.0	90.6	92.2
6	99.5	99.5	99.5	99.5	99.5		99.5	98.9	99.7	93.3	96.0	96.0	93.5	93.3	89.8	90.9	88.4	88.4	99.5	91.1	91.9
7	100.0	99.5	99.5	99.5	99.5	99.5		98.9	99.7	93.3	96.0	96.0	93.5	93.3	89.2	90.9	88.4	88.4	99.5	90.6	91.9
8	98.9	98.9	98.9	98.9	98.9	98.9	98.9		99.2	93.0	95.7	95.7	93.5	93.0	89.2	90.6	88.4	87.9	98.9	90.6	91.4
9	99.7	99.7	99.7	99.7	99.7	99.7	99.7	99.2		93.5	96.2.	96.2	93.8	93.5	89.5	91.1	88.7	88.2	99.7	90.9	92.2
10	93.3	93.3	93.3	93.3	93.3	93.3	93.3	93.0	93.5		94.1	94.4	93.3	90.9	91.7	92.7	89.8	90.3	93.3	91.4	92.2
11	96.0	96.0	96.0	96.0	96.0	96.0	96	95.7	96.2	94.1		99.5	95.4	96.0	90.6	92.2	89.2	89.8	96.0	91.4	91.1
12	96.0	96.0	96.0	96.0	96.0	96.0	96	95.7	96.2	94.4	99.5		95.4	96.5	90.9	92.2	89.2	90.1	96.0	91.4	91.1
13	93.5	93.5	93.5	93.5	93.5	93.5	93.5	93.5	93.8	93.3	95.4	95.4		92.7	89.8	90.9	89.0	88.7	93.5	90.1	89.5
14	93.3	93.3	93.3	93.3	93.3	93.3	93.3	93.0	93.5	90.9	96.0	96.5	92.7		89.0	90.3	87.9	88.2	93.3	90.1	90.3
15	89.2	89.2	89.2	89.8	89.2	89.8	89.2	89.2	89.5	91.7	90.6	90.9	89.8	89.0		97.8	94.4	90.0	89.2	97.3	90.9
16	90.9	90.9	90.9	91.4	90.9	90.9	90.9	90.6	91.1	92.7	92.2	92.2	90.9	90.5	97.4		95.4	96.5	90.9	97.8	92.5
17	88.4	88.4	88.4	89.0	88.4	88.4	88.4	88.4	88.7	89.8	89.2	89.2	89.0	87.9	94.4	95.4		94.6	88.4	94.9	88.4
18	88.4	87.9	87.9	88.4	88.2	88.4	88.4	87.9	88.2	90.3	89.8	90.1	88.7	88.2	97.0	96.5	94.6		88.2	96.5	90.1
19	99.5	99.5	99.5	99.5	100.0	99.5	99.5	98.9	99.7	93.3	96.0	86.0	93.5	93.3	89.2	90.9	88.4	88.2		90.6	92.2
20	90.6	90.6	90.6	91.1	90.6	91.1	90.6	90.6	90.9	91.4	91.4	91.4	90.1	90.1	97.3	97.8	94.9	96.5	90.6		91.9
21	91.9	91.9	91.9	92.5	92.2	91.9	91.9	91.4	92.2	92.2	91.1	91.1	89.5	90.3	80.9	92.5	88.4	90.1	92.2	91.9

PRRSV HNHK3-2021毒株的ORF5和ORF7序列分析

Sequence analysis of ORF3 and ORF5 of PRRSV HNHK3-2021 strain isolated in Hainan

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[2]	白剑宇, 宋峰惠, 吴正保, 刘正兴, 崔燕华, 史彦江. 新疆红枣软腐病病原菌的鉴定[J]. 新疆农业科学, 2017, 54(8): 1475-1480.
[3]	吴鹏;张勋;梁田;陈新凯;肖媛媛;盛金良. 北疆地区猪繁殖与呼吸综合征病毒ORF7基因克隆与遗传演化分析[J]. , 2016, 53(11): 2149-2156.
[4]	陆桂丽;汪萍;成进;夏俊;沙依兰·卡依扎;吐尔洪·努尔;王文. 猪繁殖与呼吸综合征新疆株ORF2-7基因的克隆及序列分析[J]. , 2014, 51(3): 546-551.
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