新疆农业科学 ›› 2022, Vol. 59 ›› Issue (4): 916-924.DOI: 10.6048/j.issn.1001-4330.2022.04.016
贾尊尊1,2(), 付开赟2, 丁新华2(
), 吐尔逊·阿合买提2, 姜卫华3, 王小武4, 郭文超2(
)
收稿日期:
2021-07-10
出版日期:
2022-04-20
发布日期:
2022-04-24
通信作者:
郭文超(1966-),男,河北人,研究员,博士生导师,研究方向为农业害虫综合防治,(E-mail) gwc1966@163.com;基金资助:
JIA Zunzun1,2(), FU Kaiyun2, DING Xinhua2(
), Tuerxun·Ahemaiti2, JIANG Weihua3, WANG Xiaowu4, GUO Wenchao2(
)
Received:
2021-07-10
Published:
2022-04-20
Online:
2022-04-24
Supported by:
摘要:
【目的】研究不同密度与不同为害时间下,棉花植株对烟粉虱持续取食胁迫所产生防御应答响应,分析叶片中防御酶活性,营养物质和代谢产物含量的变化,分析烟粉虱与棉花的互作关系。【方法】在人工气候室内,叶片真叶生长至5叶的棉花植株(NJ-135)上,分别接入0头(对照)、50和500头烟粉虱,单株罩140目尼龙网,在烟粉虱持续取食24、48和72 h后,分别采集棉花叶片,测定总蛋白、可溶性糖、脯氨酸、丙二醛、过氧化氢酶和叶绿素的含量。【结果】50和500头/株处理组,烟粉虱持续取食24、48和72 h,叶片中总蛋白、脯氨酸、过氧化氢酶含量均与取食时间的增长呈正相关,随取食时间和密度的加大而增高(3.23, 4.87和5.55 μg/mL, 3.84, 5.38和5.92 μg/mL; 0.85, 0.92和0.99 μg/g, 0.89, 0.98和1.21 μg/g; 9.81, 10.98和18.45 U/mg prot; 12.42, 15.29和19.16 U/mg prot),叶片中可溶性糖含量随取食时间延长逐渐下降(16.77, 15.83和15.64 mg/g; 18.10, 15.75和7.84mg/g),丙二醛含量随烟粉虱持续取食时间延长先下降后升高(3.76, 3.26和4.44 nmol/mg prot; 4.45, 3.94和5.15 nmol/mg prot),叶绿素含量变化低密度与高密度处理含量动态变化不同,50头/株为先下降后上升(1.27, 0.90和1.92 mg/g),500头/株为逐渐上升(0.50, 0.86和1.98 mg/g)。【结论】 棉花叶片会对烟粉虱取食胁迫产生相应的防御应答,与棉花生理生化性质改变有关。密度与烟粉虱持续取食时间,均是诱导叶片内总蛋白、脯氨酸、丙二醛、过氧化氢酶、可溶性糖和叶绿素含量变化的因素。高密度的烟粉虱会增强这种应答的程度,随着为害时间的延长应答程度也随之增强。
中图分类号:
贾尊尊, 付开赟, 丁新华, 吐尔逊·阿合买提, 姜卫华, 王小武, 郭文超. 烟粉虱持续取食对棉花叶片防御酶活性、营养物质和代谢产物含量的影响[J]. 新疆农业科学, 2022, 59(4): 916-924.
JIA Zunzun, FU Kaiyun, DING Xinhua, Tuerxun·Ahemaiti, JIANG Weihua, WANG Xiaowu, GUO Wenchao. The Influence of Bemisia tabaci (Gennadius) stress on the enzyme activity, nutrient and metabolite content of cotton leaves[J]. Xinjiang Agricultural Sciences, 2022, 59(4): 916-924.
[1] | 郭文超, 吐尔逊, 周桂玲, 等. 新疆农林外来生物入侵现状、趋势及对策[J]. 新疆农业科学, 2012, 49(1):86-100. |
GUO Wenchao, Tuer xun, ZHOU Guiling, et al. The Status Quo and Trend of Invasive Alien Species in Agriculture &Forest in Xinjiang,China and Relevant Countermeasures[J]. Xinjiang Agricultural Sciences, 2012, 49(1):86-100. | |
[2] | 林克剑, 张永军, 吴孔明, 等. 入侵种烟粉虱寄主选择机制和扩散能力的研究进展[A]. 农业生物灾害预防与控制研究[C]. 中国植物保护学会, 2005. |
LIN Kejian, ZHANG Yongjun, WU Kongming, et al. The Development of Host Selected Mechanism and Diffusicity of an Extic Pest of Tobacco Whitefly Bemisia tabaci Gennadius in China[C]. China Society of Plant Protection, 2005. | |
[3] |
Boykin Laura M,. Shatters Robert G. et al. Global relationships ofBemisia tabaci(Hemiptera: Aleyrodidae). revealed using Bayesian analysis of mitochondrial COI DNA sequences[J]. Molecular Phylogenetics and Evolution, 2007, 44(3):1306-1319.
PMID |
[4] | 褚栋, 潘慧鹏, 国栋, 等. Q型烟粉虱在中国的入侵生态过程及机制[J]. 昆虫学报, 2012, 55(12):1399-1405. |
CHU Dong, PAN Huipeng, GUO Dong, et al. Ecological processes and mechanisms of invasion of the alien whitefly Bemisia tabaci biotype Q in China[J]. Acta Entomologica Sinica, 2012, 55(12) : 1399-1405. | |
[5] | 刘勇, 孙玉诚, 王国红. 植物和刺吸式口器昆虫的诱导防御与反防御研究进展[J]. 应用昆虫学报, 2011, 48(4):1052-1059. |
LIU Yong, SUN Yucheng, WANG Guohong. The induced defense and anti-defense between host plant and phloem sucker insect[J]. Chinese Journal of Applied Entomology, 2011, 48(4): 1052-1059. | |
[6] | 陈婷, 汤亚飞, 赵蕊, 等. 传播木尔坦棉花曲叶病毒的烟粉虱隐种鉴定[J]. 植物保护学报, 2016, 43(1):91-98. |
CHEN Ting, TANG Yafei, ZHAO Rui, et al. Identification of the cryptic species ofBemisia tabaci transmitting cotton leaf curl Multan virus[J]. Journal of Plant Protection, 2016, 43(1): 91-98. | |
[7] | 张宝成, 白艳芬, 陈骥. 病虫害侵染对植物光合作用影响研究进展[J]. 河南师范大学学报(自然科学版), 2015, 43(2):119-125. |
ZHANG Baocheng, BAI Yanfen, CHEN Ji. Effects of Photosynjournal by Pests and Diseases: a Review[J]. Journal of Henan Normal University (Natural Science Ed.), 2015, 43(2):119-125. | |
[8] | 焦德志, 赵泽龙. 盐碱胁迫对植物形态和生理生化影响及植物响应的研究进展[J]. 江苏农业科学, 2019, 47(20):1-4. |
JIAO Dezhi, ZHAO Zelong. Effects of saline stress on plant morphology and physiology and plant response[J]. Jiangsu Agricultural Sciences, 2019, 47(20):1-4. | |
[9] | 魏秀君, 殷云龙, 芦治国, 等. NaCl胁迫对5种绿化植物幼苗生长和生理指标的影响及耐盐性综合评价[J]. 植物资源与环境学报, 2011, 20(2):35-42. |
WEI Xiujun, YIN Yunlong, LU Zhiguo, et al. Effects of NaCI stress on growth and physiological indexes of five greening plant seedlings and comprehensive evaluation of their salt tolerance[J]. Journal of Plant Resources and Environment, 2011, 20(2):35-42. | |
[10] | 张国盛, 郝蕾, 闫子娟, 等. 6种树种叶片叶绿素荧光动力学参数对土壤水分变化的响应[J]. 生态学杂志, 2017, 36(11):3079-3085. |
ZHANG Guosheng, HAO Lei, YAN Zijuan, et al. The responses of chlorophyll fluorescence kinetics parameters of six tree species to soil moisture changes[J]. Chinese Journal of Ecology, 2017, 36(11): 3079-3085. | |
[11] | 蒲恒浒, 郅军锐, 曾广, 等. 不同诱导处理对番茄叶片营养物质含量及防御酶活性的影响[J]. 环境昆虫学报, 2019, 41(2):405-412. |
PU Henghu, ZHI Junrui, ZENG Guang, et al., Effects of different induction treatments on contents of nutrient and activities of defense enzymes in the leaves of tomato plant[J]. Journal of Environmental Entomology, 2019, 41(2):405-412. | |
[12] | 李艳艳, 周晓榕, 庞保平, 等. 瓜蚜为害对寄主植物主要营养物质和次生物质的影响[J]. 环境昆虫学报, 2013, 35(1):49-54. |
LI Yanyan, ZHOU Xiaorong, PANG Baoping, et al. Influences of Aphis gossypii Glover feeding on the contents of main nutrients and secondary compounds in host plants[J]. Journal of Environmental Entomology, 2013, 35(1):49-54. | |
[13] | 钱蕾, 蒋兴川, 刘建业, 等. 大气CO2浓度升高对西花蓟马生长发育及其寄主四季豆营养成分的影响[J]. 生态学杂志, 2015, 34(6):1553-1558. |
QIAN Lei, JIANG Xingchuan, LIU Jianye, et al. Effect of elevated CO2 concentration on development of the western flower thrips,Frankliniella occidentalis (Thysanoptera: Thripidae) and nutrients of their host plant,Phaseolus vulgaris[J]. Chinese Journal of Ecology, 2015, 34(6):1553-1558. | |
[14] | 曹家畅, 周倩, 龚明. 细胞氧化还原状态在植物对干旱胁迫响应与适应中的作用[J]. 安徽农业科学, 2018, 46(28):6-10. |
CAO Jiachang, ZHOU Qian, GONG Ming. Roles of Intracellular Redox State in Response and Adaptation of Plants to Drought Stress[J]. J.Anhui Agric. Sci. 2018, 46(28):6-10. | |
[15] | Zhang X, Cui L, Wang B, et al. Effect of soil drought stress on the lipid peroxidation and the activity of protective enzyme in Larrea tridentate[J]. Ecology and Environmental Sciences, 2010. |
[16] | 沙品洁, 亓永凤, 吕超, 等. 棉铃虫持续取食对棉花三种防御酶活性的作用[J]. 应用昆虫学报, 2012, 49(2):414-421. |
SHA Pinjie, QI Yongfeng, LÜ Chao, et al. The effects of Helicoverpa armigera herbivory on the activity of phenylanlanine ammonialyase,lipoxygenase and polyphenol oxidase in cotton seedling leaves[J]. Chinese Journal of Applied Entomology, 2012, 49(2):414-421. | |
[17] | 梁晓, 卢芙萍, 卢辉, 等. 保护酶CAT在木薯种质抗螨中的功能初步研究[J]. 热带作物学报, 2017, 38(2):343-348. |
LIANG Xiao, LU Fuping, LU Hui, et al. Preliminary Study of The Function of Catalase in Cassava Resistance to Mite[J]. Chinese Journal of Tropical Crops, 2017, 38(2):343-348. | |
[18] | 钦俊德, 王琛柱. 论昆虫与植物的相互作用和进化的关系[J]. 昆虫学报, 2001,(3):360-365. |
QIN Junde, WANG Chenzhu. The relation of interaction between inects and plants to evolution[J]. Acta Entomaologica sinica, 2001,(3):360-365. | |
[19] | 秦秋菊, 高希武. 昆虫取食诱导的植物防御反应[J]. 昆虫学报, 2005,(1):125-134. |
QIN Qiuju, GAO Xiwu. Plant defense responses induced by insect herbivory[J]. Acta Entomologica Sinica, 2005,(1):125-134. | |
[20] | Tscharntke T, Thiessen S, Dolch R, et al. Herbivory, induced resistance, and interplant signal transfer in Alnus glutinosa[J]. Biochemical Systematics & Ecology, 2001, 29(10):1025-1047. |
[21] |
Kerchev, P I, Fenton, et al. Plant responses to insect herbivory: interactions between photosynjournal, reactive oxygen species and hormonal signalling pathways[J]. Plant Cell Environ, 2012, 35(2):441-453.
DOI URL |
[22] | 贾尊尊, 王小武, 付开赟, 等. 新疆主要农区烟粉虱生物型鉴定及其对11种常用杀虫剂的抗性监测[J]. 新疆农业科学, 2017, 54(2):304-312. |
JIA Zunzun, WANG Xiaowu, FU Kaiyun, et al. Biotype identification and resistance status of Bemisiatabaci from Xinjiang Agriculture areas[J]. Xinjiang Agricultural Sciences, 2017, 54(2):304-312. | |
[23] | Wang Q, Su Z, Zhang S, et al. Soluble sugar content of clonal plant Neosinocalamus affinis at module and ramet levels[J]. Ying Yong Sheng Tai Xue Bao, 2004, 15(11):1994-1998. |
[24] | Hai Ling L I, Peng S M, Lin L I, et al. Studies on four conventional methods for protein determination[J]. Chinese Journal of Biochemical Pharmaceutics, 2008, 29(4):277-276. |
[25] | Abrahám E, Hourton-Cabassa C, Erdei L, et al. Methods for Determination of Proline in Plants[J]. Methods Mol Biol, 2015, 639:317-331. |
[26] |
Vernon L P. Spectrophotometric Determination of Chlorophylls and Pheophytins in Plant Extracts[J]. Analytical Chemistry, 1960, 32(9):1414-1414.
DOI URL |
[27] | Akkaya H, Yilmaz O. Antioxidant Capacity and Radical Scavenging Activity of Silybum marianum and Ceratonia siliqua[J]. Ekoloji, 2012, 21(82):9-16. |
[28] | 彭建, 王丹英, 徐春梅, 等. 钼酸铵法测定水稻过氧化氢酶活性[J]. 中国农学通报, 2009, 25(16):61-64. |
PENG Jian, WANG Danying, XU Chunmei, et al., Ammonium Molybdate Method for Detecting the Activities of Rice Catalase[J]. Chinese Agricultural Science Bulletin, 2009, 25(16):61-64. | |
[29] | 温娟, 郅军锐, 吕召云, 等. 二斑叶螨为害对番茄叶片主要营养物质和防御酶活性的影响[J]. 环境昆虫学报, 2017, 39(1):172-180. |
WEN Juan, ZHI Junrui, LÜ Zhaoyun, et al. Effects of Tetranychus urticae feeding on the contents of main nutrient and defensive enzymes activities of tomato leaves[J]. Journal of Environmental Entomology, 2017, 39(1):172-180. | |
[30] | 许冬. 斜纹夜蛾取食诱导棉花抑制性消减文库的构建及初步分析[D]. 北京:中国农业科学院, 2008. |
XU Dong. Construcrion and preliminary analysis of suppression subtractive hybridization library for cotton induced By Spodoptera Litura[D]. Beijing: Chinese Academy of Agricultural Sciences, 2008. | |
[31] | 张坤鹏, 孙瑞红, 杨洪强. 植物对叶螨侵害的生理生化反应的研究进展[J]. 中国农学通报, 2013, 29(36):334-337. |
ZHANG Kunpeng, SUN Ruihong, YANG Hongqiang. The Progress of Physiological and Biochemical Reaction of Plants to Pest Mites Damage[J]. Chinese Agricultural Science Bulletin, 2013, 29(36):334-337. | |
[32] | 罗丹, 张喜春, 田硕. 低温胁迫对番茄幼苗脯氨酸积累及其代谢关键酶活性的影响[J]. 中国农学通报, 2013, 29(16):90-95. |
LUO Dan, ZHANG Xichun, TIAN Shuo. Effect of Low Temperature Stress on Proline Accumulation and the Activities of the Key Enzymes Involved in the Proline Metabolism in Leaves of Tomato Seedling[J]. Chinese Agricultural Science Bulletin, 2013, 29(16):90-95. | |
[33] | 马博英. 多年生黑麦草的逆境生理研究进展[J]. 生物学杂志, 2010, 27(2):58-61. |
MA Boying. Research advance in stress physiological adaptation of perennial ryegrass[J]. Journal of Biology, 2010, 27(2):58-61. | |
[34] |
Merino-Cabrera, Zanuncio, J C, et al. Biochemical response between insects and plants: an investigation of enzyme activity in the digestive system of Leucoptera coffeella (Lepidoptera: Lyonetiidae) and leaves of Coffea arabica (Rubiaceae) after herbivory[J]. ANN APPL BIOL, 2018, 172(2):236-243.
DOI URL |
[35] |
Radi R, Beckman J S, Bush K M, et al. Peroxynitrite-induced membrane lipid peroxidation: The cytotoxic potential of superoxide and nitric oxide[J]. Archives of Biochemistry and Biophysics, 1991, 288(2):481-487.
PMID |
[36] | Radi R, Turrens J F, Freeman B A. Cytochrome c-catalyzed membrane lipid peroxidation by hydrogen peroxide[J]. Archives of Biochemistry & Biophysics, 1991, 288(1):118-125. |
[37] | 徐萍, 李进, 吕海英, 等. 干旱胁迫对银沙槐幼苗叶绿体和线粒体超微结构及膜脂过氧化的影响[J]. 干旱区研究, 2016, 33(1):120-130. |
XU Ping, LI Jin, LÜ Haiying, et al. Effects of Drought Stress on Ultrastructure of Chloroplast and Mitochondria and Membrane Lipid Peroxidation of Ammodendron argenteum[J]. Arid Zone Research, 2016, 33(1):120-130. | |
[38] | 周艳, 刘慧英, 王松, 等. 外源GSH对盐胁迫下番茄幼苗生长及抗逆生理指标的影响[J]. 西北植物学报, 2016, 36(3):515-520. |
ZHOU Yan, LIU Huiyin, WANG Song, et al. Effect of Exogenous GSH on Tomato Seedlings Growth and Physiological Indexes of Resistance Stress under Salt Stress[J]. Acta Botanica Boreali-Occidentalia Sinica, 2016, 36(3):515-520. | |
[39] | 郭祖国, 王梦馨, 崔林, 等. 6种防御酶调控植物体应答虫害胁迫机制的研究进展[J]. 应用生态学报, 2018, 29(12):4248-4258. |
GUO Zuguo, WANG Mengxin, CUI Lin, et al. Research progress on the underlying mechanisms of plant defense enzymes in response to pest stress[J]. Chinese Journal of Applied Ecology, 2018, 29(12):4248-4258. |
[1] | 陈文斌, 刘喆, 刘宗泉, 仇保华, 杨益众. 烟粉虱对3种寄主植物的田间和室内趋性[J]. 新疆农业科学, 2021, 58(11): 2049-2055. |
[2] | 吕欢欢, 周成松, 杨柳, 刘祯妍, 马德英. MEAM1烟粉虱隐种对3种新烟碱类杀虫剂交互抗性及交互现实遗传力的分析[J]. 新疆农业科学, 2020, 57(9): 1734-1745. |
[3] | 顾爱祥,周福才,杨益众,苏宏华. 棉田烟粉虱的为害及其经济阈值分析[J]. 新疆农业科学, 2019, 56(1): 67-73. |
[4] | 张鑫, 王文卿, 靳改龙, 张祥, 马德英. 两种烟熏剂对设施蔬菜上烟粉虱及海氏桨角蚜小蜂(Eretmocerus hayati)的毒力测定[J]. 新疆农业科学, 2017, 54(8): 1469-1474. |
[5] | 贾尊尊;王小武;付开赟;丁新华;郭文超;吐尔逊·阿合买提;姜卫华;卡德·艾山. 新疆主要农区烟粉虱生物型鉴定及其对11种常用杀虫剂的抗性监测[J]. , 2017, 54(2): 304-312. |
[6] | 何伟, 杨华, 许建军, 孙晓军. 脉冲式烟雾机在温室番茄应用防治番茄主要病虫害效果评价[J]. 新疆农业科学, 2017, 54(12): 2255-2261. |
[7] | 买热木古丽·克依木;徐好学;鲜金花;李晶;王惠卿;胡爱芝;马德英. 5种环境友好杀虫剂对MEAM1烟粉虱隐种的药效评价[J]. , 2014, 51(6): 1137-1142. |
[8] | 买热木古丽·克依木;李晶;王惠卿;古丽扎热·帕热合提;马德英. 新疆地区烟粉虱携带番茄黄化曲叶病毒动态检测[J]. , 2014, 51(3): 455-463. |
[9] | 热孜万古丽·阿布都哈尼;买合甫皮古丽·阿不力米提;热孜万古丽·加马力;张燕南;李京;马德英. 枣棉套作田烟粉虱及优势寄生蜂桨角蚜小蜂的种群动态及空间分布规律[J]. , 2013, 50(10): 1842-1849. |
[10] | 陈丹;张以和;热孜万古力;米日古丽;杨媚;潘卫萍;马德英. 黄板诱杀对设施蔬菜烟粉虱控制作用研究[J]. , 2012, 49(2): 255-260. |
[11] | 陈丹;王玉波;王惠;潘卫萍;吉艳玲;曾继勇;马德英. 丽蚜小蜂与黄板联合使用对设施番茄烟粉虱的控制作用研究[J]. , 2011, 48(5): 841-847. |
[12] | 吴刚;王登元;王希东. 分子标记检测棉田烟粉虱捕食性天敌种类的研究[J]. , 2011, 48(2): 223-228. |
[13] | 李杰;于江南;王登元;王惠杰. 烟粉虱在棉田的分布规律研究[J]. , 2010, 47(9): 1892-1896. |
[14] | 段晓东;张新;冯明星;马德英. 新疆B型烟粉虱对不同类型杀虫剂的敏感性测定与分析[J]. , 2010, 47(7): 1343-1347. |
[15] | 张仁福;于江南;王登元;李杰. 吐鲁番地区烟粉虱寄主种类与危害程度调查[J]. , 2009, 46(4): 730-735. |
阅读次数 | ||||||||||||||||||||||||||||||||||||||||||||||||||
全文 54
|
|
|||||||||||||||||||||||||||||||||||||||||||||||||
摘要 302
|
|
|||||||||||||||||||||||||||||||||||||||||||||||||