应用RP-HPLC法测定野山杏果肉黄酮类化合物

doi:10.6048/j.issn.1001-4330.2021.08.014

新疆农业科学 ›› 2021, Vol. 58 ›› Issue (8): 1476-1485.DOI: 10.6048/j.issn.1001-4330.2021.08.014

• 植物保护·园艺特产·生理生化·农业装备工程与机械化 • 上一篇下一篇

应用RP-HPLC法测定野山杏果肉黄酮类化合物

戴小华, 谷虹霏, 金科旭, 束佳敏, 杨若蓝, 吴梦岚, 潘慧, 赵红琼

新疆农业大学,乌鲁木齐 830052

收稿日期:2020-06-15 出版日期:2021-08-20 发布日期:2021-08-09
作者简介:戴小华(1976-),女,广西人,副教授,博士,研究方向为天然产物功能性, (E-mail)254799258@qq.com
基金资助:
国家自然科学基金(31960718);新疆维吾尔自治区科技支疆项目(2017E0242);新疆农业大学大学生创新项目(201710758172)

Determination of Flavonoids in Wild Apricot Flesh by RP-HPLC

DAI Xiaohua, GU Hongfei, JIN Kexu, SHU Jiamin, YANG Ruolan, WU Menglan, PAN Hui, ZHAO Hongqiong

Xinjiang Agriculture University, Urumqi 830052, China

Received:2020-06-15 Published:2021-08-20 Online:2021-08-09
Supported by:
National Natural Science Foundation of China 31960718,Science and technology support project of Xinjiang Uygur Autonomous Region 2017E0242,Xinjiang Agricultural University Student Innovation Project 201710758172

摘要/Abstract

摘要： 【目的】分析测定新疆野山杏果肉中4种黄酮类化合物(原花青素、芦丁、柚皮苷二氢查尔酮、槲皮素)。【方法】运用高效液相色谱分析方法,采用Hubble C18(4.6 mm×250 mm,5 μm)色谱柱,流动相A(甲醇): B(0.5%磷酸水溶液)= 45∶55,在1.0 mL/min流速下等度洗脱,分离新疆野山杏果肉中4种黄酮类化合物,采用仪器精密度、稳定性、重复性及添加回收率验证该方法。【结果】原花青素、芦丁、槲皮素3种黄酮成分色谱峰在20 min内分离度良好,线性范围为0.52～56 μg/mL(r = 0.999 8～1),加样回收率为92.13%～96.70%,RSD为0.89%～1.96%;原花青素含量为7.325 mg/kg,芦丁含量为0.630 mg/kg,槲皮素含量为0.009 58 mg/kg,柚皮苷二氢查尔酮在该条件下未检出。【结论】高效液相色谱(RP-HPLC)简便、快速、准确、稳定性及重复性好,适用于新疆野山杏果肉中芦丁等3种黄酮类化合物的分离测定。

关键词: 野山杏; 黄酮类化合物; 反相高效液相色谱

Abstract: 【Objective】 To establish a method for simultaneous determination of four flavonoids (proanthocyanidins, rutin, naringin dihydrochalcone and quercetin) in the sarcocarp of Xinjiang wild apricot by high performance liquid chromatography.【Method】 Four flavonoids from Xinjiang wild apricot sarcocarp were separated by Hubble C18 (4.6 mm ×250 mm, 5 m) column, mobile phase A (methanol)∶ B (0.5% phosphoric acid solution) = 45∶55, elution at a flow rate of 1.0 mL/min. The method was validated by instrument precision, stability, repeatability and recovery.【Result】 The chromatographic peaks of proanthocyanidin, rutin and quercetin were well separated within 20 minutes. The linear range was 0.52-56 g/mL (r = 0.999,8-1). The recovery was 92.13%-96.70% and RSD was 0.89%-1.96%. The content of proanthocyanidins, rutin and quercetin were 7.325 mg/kg, 0.630 mg/kg and 0.009,58 mg/kg respectively. Naringin dihydrochalcone was not detected under this condition.【Conclusion】 The method is simple, rapid, accurate, stable and reproducible. It is suitable for the separation and determination of three flavonoids in Xinjiang wild apricot flesh.

Key words: wild apricot; flavonoids; RP-HPLC

中图分类号:

S662.2

戴小华, 谷虹霏, 金科旭, 束佳敏, 杨若蓝, 吴梦岚, 潘慧, 赵红琼. 应用RP-HPLC法测定野山杏果肉黄酮类化合物[J]. 新疆农业科学, 2021, 58(8): 1476-1485.

DAI Xiaohua, GU Hongfei, JIN Kexu, SHU Jiamin, YANG Ruolan, WU Menglan, PAN Hui, ZHAO Hongqiong. Determination of Flavonoids in Wild Apricot Flesh by RP-HPLC[J]. Xinjiang Agricultural Sciences, 2021, 58(8): 1476-1485.

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应用RP-HPLC法测定野山杏果肉黄酮类化合物

Determination of Flavonoids in Wild Apricot Flesh by RP-HPLC

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