Abstract: [Objective] This project aims to analyze the changes of blood biochemical indexes in different training stages and explore the feasibility of training program in order to provide data reference for training and training of young Yili horse through the training program for 8 young Yili horses, a training program combining ramp training with interval training for 6 weeks.[Method]Testing plasma with ALT, AST, ALT/AST, CK, LDH, CREA,TG, TP,UA, LAC, Ca2+ and P+, twelve blood biochemical indexes at week 0, week 2, week 4 and week 6, the training respectively and analyzing the variance, and contrast the changing rule of the during different training stages.[Result]The activities of ALT and ALT/AST were significantly higher than those before training at 4th week (P<0.05), TG and Ca2+ were significantly lower than those before training at 6 th week (P<0.05).After game, the level of ALT, ALT/AST and CREA training were extremely significant higher than those before training at 4 th week (P<0.01).At the 6th week, LAC was significantly higher than that before training, Ca2+ was significantly lower than that before training (P<0.05).[Conclusion]This indicated that the experiment used in the tuning training program of the test had a positive effect and its anaerobic metabolic capacity has been increased.The training program can be used as a reference scheme for future horse tuning training.