新疆红枣软腐病病原菌的鉴定

doi:10.6048/j.issn.1001-4330.2017.08.013

新疆农业科学 ›› 2017, Vol. 54 ›› Issue (8): 1475-1480.DOI: 10.6048/j.issn.1001-4330.2017.08.013

新疆红枣软腐病病原菌的鉴定

白剑宇^1,2, 宋峰惠², 吴正保², 刘正兴³, 崔燕华³, 史彦江²

1.新疆农业大学林学与园艺学院博士流动站,乌鲁木齐 830052;
2.新疆林业科学院经济林研究所,乌鲁木齐 830063;
3.阿克苏地区农业技术推广中心,新疆阿克苏 843000

收稿日期:2017-06-09 发布日期:2020-07-22
通信作者: 史彦江(1961-),男,山西人,研究员,研究方向为林木引种育种,(E-mail)syj504@126.com
作者简介:白剑宇(1977-),男,河北承德人,副研究员,博士后,研究方向为林业有害生物防控,(E-mail)bjyhao2010@sina.com
基金资助:
中国博士后科学基金面上项目“新疆红枣黑斑病菌的田间追踪检测技术研究”(2014M552565XD);新疆维吾尔自治区林业发展补助资金项目“南疆红枣主产区红枣主要病害普查鉴定及田间追踪监测技术研究”(XJLKY2016056)

Identification of the Pathogen of Jujube Soft Rot in Xinjiang

BAI Jian-yu^1,2, SONG Feng-hui², WU Zheng-bao², LIU Zheng-xing³, CUI Yan-hua³, SHI Yan-jiang²

1. Postdoctoral Mobile Research Station of College of Forestry and Horticulture,Urumqi 830052, China;
2. Research Institute of Economic Forestry,Xinjiang Academy of Forestry Sciences,Urumqi 830063,China;
3. Agricultural Technology Extension Center of Aksu Prefecture, Aksu Xinjiang 843000, China

Received:2017-06-09 Published:2020-07-22
Correspondence author: SHI Yan-jiang, (1961- ), male, native place: Shanxi. Professor, research field: Introduction and breeding of forest trees, (E-mail) syj504@126. Com
Supported by:
China Postdoctoral Science Foundation Project "The Tracking and Detection Technology of Pathogen of the Black Spot of Jujube in Fields in Xinjiang" (2014M552565XD) and Xinjiang Uygur Autonomous Region Research Institute Fund Project "Study on the Monitoring Technology of Screening and Identification of Main Diseases and Field Tracking in the Main Jujube Producing Areas in Southern Xinjiang" (KYGY2016055)

摘要/Abstract

摘要： 【目的】鉴定枣软腐病的致病病原,为有针对性的开展病害防治奠定基础。【方法】采用病原菌常规分离技术获取疑似病原物,通过形态学特征和回接验证对病原进行鉴定;利用PCR技术获取病原菌的ITS基因的序列片段,通过与GenBank中已有的相关序列进行比对分析,构建遗传进化树,结合形态学鉴定和致病性实验验证,鉴定病原。【结果】新疆枣软腐病病原的形态学特征与米根霉(Rhizopus oryzae)相似。枣软腐病的病原与GenBank中已知的米根霉菌株的ITS序列显示99%~100%的相似性,在遗传进化树分析中并聚在同一组。【结论】基于形态学特征、致病性试验以及分子鉴定结果,新疆枣软腐病病原被鉴定为Rhizopus oryzae。

关键词: 枣软腐病; rDNA-ITS; 病原鉴定; 遗传进化树分析

Abstract: 【Objective】 The pathogen of soft rot disease of jujube was accurately identified so as to lay a foundation for disease prevention and control. 【Method】The suspected pathogens were obtained by the conventional isolation technology and were preliminarily identified by morphological features and loop validation. The fragments of ITS gene of the suspected pathogens were obtained by the PCR technique. When the related trains of Rhizopus oryzae in GenBank were compared and analyzed, the genetic evolutionary tree was constructed and the pathogen was identified by morphological identification and pathogenicity experiments.【Result】According to the morphological characteristics and the pathogenicity test results, the morphological characteristics of Rhizopus soft rot pathogen of Xinjiang jujube were similar to that of Rhizopus oryzae. The phylogenetic analysis of the pathogen showed that the ITS gene of pathogen of jujube soft rot showed 99 % to 100 % similarity with part ITS gene of Rhizopus oryzae in GenBank, and in the analysis of phylogenetic tree, they were clustered in the same group.【Conclusion】Based on the morphological characteristics, pathogenicity test and molecular identification of the pathogen, the pathogen of jujube soft rot was identified as Rhizopus oryzae.

Key words: jujube soft rot; rDNA-ITS; pathogens identification; phylogenetic analysis

中图分类号:

S436.64

白剑宇, 宋峰惠, 吴正保, 刘正兴, 崔燕华, 史彦江. 新疆红枣软腐病病原菌的鉴定[J]. 新疆农业科学, 2017, 54(8): 1475-1480.

BAI Jian-yu, SONG Feng-hui, WU Zheng-bao, LIU Zheng-xing, CUI Yan-hua, SHI Yan-jiang. Identification of the Pathogen of Jujube Soft Rot in Xinjiang[J]. Xinjiang Agricultural Sciences, 2017, 54(8): 1475-1480.

参考文献

[1] 刘孟军, 汪民.中国枣种质资源[M].北京: 中国林业出版社, 2009: 56-57.
   LIU Meng-jun, WANG Min. (2009). Chinese Jujube Germplasm Resources [M]. Beijing: China Forestry Press: 56-57. (in Chinese)
[2] Bai, J. Y., Guo, Q. Y., Wang, X. M., & Duan, C. X. (2015). First report of jujube leaf spot caused by alternaria alternata in China. Plant Disease, 99(11): 1,624.
[3] Bai, J. Y., Wang, X., Shi, Y., & Duan, C. (2016). Occurrence and identification of nothophoma quercina causing brown spot of jujube in China. Canadian Journal of Plant Pathology, 38(4): 527-532.
[4] Bai, J. Y., Guo, Q. Y., Song, F. J., Liu, Z. X., Liu, Y., & Lei, C. J. (2015). First report of Alternaria alternate causing brown spot of zizyphus jujuba in China. Journal of Plant Pathology, 97(2): 55-57.
[5] 雷春军,黄晓玲,刘正兴,等.阿克苏地区骏枣储藏期致腐病原菌种类鉴定[J].新疆农业科技,2014,(4):16-17.
   LEI Chun-jun, HUANG Xiao-ling, LIU Zheng-xing, et al.(2014). Identification of pathogens species of jun jujube in storage period of fruits in Aksu, Xinjiang [J]. Xinjiang Agricultural Science and Technology, (4):16-17. (in Chinese)
[6] 沙娜瓦尔·色买提,玉山江·买买提,郭庆元,等. 枣果霉烂病病原鉴定(一):引起新疆枣果霉烂病的几种曲霉菌的分离鉴定[J].新疆农业科学,2016,53(3):502-509.
   Shanawaer Semaiti, Yushanjiang Maimaiti, GUO Qing-yuan, et al. (2016). Identification of the pathogen causing jujube fruit mildew (Part I): Isolation and identification of Aspergillus fungus causing jujube fruit mildew [J]. Xinjiang Agricultural Sciences, 53(3):502-509. (in Chinese)
[7] 沙娜瓦尔·色买提,玉山江·买买提,郭庆元,白剑宇. 枣果霉烂病病原鉴定(一):引起新疆枣果霉烂病的几种青霉菌的分离鉴定[J].新疆农业科学,2016,53(4):698-705.
   Shanawaer Semaiti, Yushanjiang Maimaiti, GUO Qing-yuan, et al. (2016). Identification of the pathogen causing jujube fruit mildew (Part II): Isolation and identification of Penicillium fungus causing jujube fruit mildew [J]. Xinjiang Agricultural Sciences, 53(4):698-705. (in Chinese)
[8] 王叶.枣果实病原真菌的鉴定及链格孢属病原真菌多样性研究[D].郑州:河南农业大学硕士论文,2011.
   WANG Ye.(2011). The identification of pathogen fungi causing jujube fruit diseases and diversity of Alternaria pathogens [D]. Master Thesis. Henan Agricultural University, Zhengzhou. (in Chinese)
[9] 张晓申.枣烂果病种类及防治措施 [J].北京农业,2008,(9) :31.
   ZHANG Xiao-shen. (2008). Species and control measures of jujube fruit rot [J]. Beijing Agriculture, (9): 31. (in Chinese)
[10] 王学军,俎文芳,王广鹏. 枣烂果病的发生原因及防治措施[J].河北果树,2002,(5):52-53.
   WANG Xue-jun, ZU Wen-fang, WANG Guang-peng. (2002).The occurrence reason and control measures of jujube fruit rot [J]. Hebei Fruit Tree, (5):52-53. (in Chinese)
[11] 柴全喜, 宋素智. 综防冬枣贮存期病害[N].河北科技报,2013,10(15):B06.
   CHAI Quan-xi, SONG Su-zhi. (2013).The integrated control of winter jujube in storage period of fruits [N]. Hebei Science and Technology Newspaper, 10(15):B06. (in Chinese)
[12] 魏景超.中国真菌志:根霉属[M]. 北京: 科学出版社, 1979: 62- 66.
   WEI Jing-chao. (1979). Chinese Journal of fungi: Alternaria [M]. Beijing: Science Press: 62-66. (in Chinese)
[13] White, T. J., Bruns, T. D., Lee, S. B., Taylor, J. W., Innis, M. A., & Gelfand, D. H., et al. (1990). Amplification and Direct Sequencing of Fungal Ribosomal RNA Genes for Phylogenetics. PCR Protocols.
[14] de Hoog, G. S., & Ah, G. V. D. E. (1998). Molecular diagnostics of clinical strains of filamentous basidiomycetes. Mycoses, 41(5-6): 183-189.

[1]	赵志强, 张卢慧, 赵全新, 郭庆元. 新疆发现甜菜新病害甜菜黄萎病[J]. 新疆农业科学, 2022, 59(2): 425-432.
[2]	李番, 任毓忠, 焦瑞莲, 孙璘, 李国英. 石竹叶斑病病原鉴定[J]. 新疆农业科学, 2021, 58(4): 712-719.
[3]	海艳, 李妍, 方治豪, 王凯涛, 任毓忠. 石河子市区大花金鸡菊白粉病病原鉴定及发病规律[J]. 新疆农业科学, 2019, 56(11): 2072-2078.
[4]	白剑宇;刘艳祥;刘正兴;崔燕华;史彦江. 阿克苏地区枣黑斑病病原鉴定及室内药剂初选[J]. , 2016, 53(9): 1674-1682.
[5]	沙娜瓦尔·色买提;玉山江·买买提;郭庆元;白剑宇. 枣果霉烂病病原鉴定(二)——引起新疆枣果霉烂病的几种青霉菌的分离鉴定[J]. , 2016, 53(4): 698-705.
[6]	沙娜瓦尔·色买提;玉山江·买买提;郭庆元;白剑宇. 枣果霉烂病病原鉴定(一)——引起新疆枣果霉烂病的几种曲霉菌的分离鉴定[J]. , 2016, 53(3): 502-509.
[7]	郭开发;姚兆群;吴彩兰;向本春;赵思峰. 新疆4种林木腐烂病菌PCR快速检测技术研究[J]. , 2016, 53(10): 1843-1849.
[8]	岳永亮;任毓忠;张莉;金恭玺;王成成;李国英. 新疆绿豆黄萎病病原菌鉴定[J]. , 2015, 52(8): 1477-1480.
[9]	王丽丽;符桂华;马金贵;日孜旺古丽;郎玉瑶;帕提古丽;李克梅. 乌昌地区马铃薯黄萎病菌分离与鉴定[J]. , 2014, 51(4): 667-672.
[10]	杜琴;孔利;赵思峰;吴彩兰;张萍. 新疆库尔勒香梨腐烂病病原鉴定[J]. , 2013, 50(12): 2258-2265.
[11]	何刚;徐彪;张利莉. 新疆白车轴草白粉病病原鉴定[J]. , 2012, 49(7): 1238-1243.
[12]	张国丽;任毓忠;赵文;张莉;李国英. 新疆加工番茄溃疡病的发生及其病原菌研究[J]. , 2011, 48(5): 848-852.
[13]	黄素芳;向本春;任毓忠;李虎. 新疆甘草斑点病病原分离鉴定[J]. , 2009, 46(3): 536-539.
[14]	付文君;夏正汉;陈蓉;李勤忠. 伊犁河谷地区玉米疯顶病发生及防治[J]. , 2007, 44(z2): 188-189.
[15]	陈卫民;马俊义;缪卫国;夏正汉;焦子伟;陈蓉;付文君;韩乃勇. 新疆向日葵白锈病与防治[J]. , 2004, 41(5): 361-362.

新疆红枣软腐病病原菌的鉴定

Identification of the Pathogen of Jujube Soft Rot in Xinjiang

PDF下载

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics