石竹叶斑病病原鉴定

doi:10.6048/j.issn.1001-4330.2021.04.014

新疆农业科学 ›› 2021, Vol. 58 ›› Issue (4): 712-719.DOI: 10.6048/j.issn.1001-4330.2021.04.014

• 土壤肥料·植物保护·微生物 • 上一篇下一篇

石竹叶斑病病原鉴定

李番, 任毓忠, 焦瑞莲, 孙璘, 李国英

新疆绿洲农业病虫害治理与植保资源利用自治区高校重点实验室/石河子大学农学院,新疆石河子 832003

收稿日期:2020-04-15 出版日期:2021-04-20 发布日期:2021-04-25
通信作者: 任毓忠(1970-),男,新疆人,副教授,研究方向为植物病害防治,(E-mail)404350315@qq.com
作者简介:李番(1995-),女,甘肃人,硕士研究生,研究方向为植物病理学,(E-mail)1986689330@qq.com
基金资助:
国家自然科学基金(31160357);兵团林业局项目(KC009903)

Identification of the Pathogen Causing Leaf Spot of Dianthus chinensis

LI Fan, REN Yuzhong, JIAO Ruilian, SUN Lin, LI Guoying

Key Laboratory for Oasis Agricultural Pest Management and Plant Resource Utilization at Universities Levels of Xinjiang Uygur Autonomous Region/College of Agronomy, Shihezi University, Shihezi Xinjiang 832003,China

Received:2020-04-15 Online:2021-04-20 Published:2021-04-25
Correspondence author: REN Yuzhong(1970-),male,from Xinjiang,associate professor,doctor,research direction is plant disease epidemic and prevention research,(E-mail)404350315@qq.com
Supported by:
National Natural Science Foundation of China(31160357);the Project of Forestry Bureau XPCC(KC009903)

摘要/Abstract

摘要： 【目的】 研究鉴定引起新疆石河子地区石竹叶斑病的病原,为石竹叶斑病的防治提供理论基础。【方法】 采集典型石竹叶斑病发病叶片利用常规组织法分离和纯化,选取8个代表性菌株,采用菌丝块贴接法和喷雾法测定致病性;应用病菌形态学和rDNA-ITS区、组蛋白3和β-微管蛋白序列进行比对和分析,建立多基因联合系统发育树,确定病原菌的分类地位。【结果】 经形态学鉴定其分生孢子与Alternaria nobilis相似;供试菌株rDNA-ITS区和β-微管蛋白序列与已报道的石竹链格孢(A. nobilis)同源性高达99.0%以上,rDNA-ITS区和β-微管蛋白序列联合构建的系统发育树显示,8个代表菌株均与A. dianthi处于同一分支上,与其它链格孢亲缘关系较远。【结论】 引起石河子地区石竹叶斑病的病原菌为石竹链格孢Alternaria nobilis。

关键词: 石竹叶斑病; 多基因联合; 病原鉴定

Abstract: 【Objective】 The aim of this project is to clarify the pathogen of Dianthus chinensis leaf spot in Shihezi area and control the disease. 【Methods】 Using conventional organic law, 8 representative strains were selected and their pathogenicity was determined by mycelium patch and spray method. The morphological and rDNA-ITS regions, histone 3 and β- tubulin sequences were compared and analyzed to establish a multi-gene combined phylogenetic tree to determine the classification status of pathogens. 【Results】 Morphologically, the conidium was similar to Alternaria nobilis. The sequence analysis of rDNA-ITS and β-tubulin of 8 isolates were more than 99.0% homologous to the reported phylogeny of Alternaria nobilis in GenBank. 【Conclusion】 Combined with the morphological characteristics and molecular biological results, the pathogen causing the Dianthus chinensis leaf spot is identified as Alternaria. nobilis in Shihezi area.

Key words: Dianthus chinensis; multi-gene analysis; pathogen identification

中图分类号:

S432.9

李番, 任毓忠, 焦瑞莲, 孙璘, 李国英. 石竹叶斑病病原鉴定[J]. 新疆农业科学, 2021, 58(4): 712-719.

LI Fan, REN Yuzhong, JIAO Ruilian, SUN Lin, LI Guoying. Identification of the Pathogen Causing Leaf Spot of Dianthus chinensis[J]. Xinjiang Agricultural Sciences, 2021, 58(4): 712-719.

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石竹叶斑病病原鉴定

Identification of the Pathogen Causing Leaf Spot of Dianthus chinensis

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