奇台窖藏马铃薯病害病原鉴定

doi:10.6048/j.issn.1001-4330.2020.05.014

新疆农业科学 ›› 2020, Vol. 57 ›› Issue (5): 902-909.DOI: 10.6048/j.issn.1001-4330.2020.05.014

奇台窖藏马铃薯病害病原鉴定

吕卓^1,2, 高勇刚³, 唐琦勇², 楚敏², 顾美英², 朱静², 程思琦¹, 马晓雪¹, 宋素琴², 王静¹

1.新疆农业大学食品科学与药学学院,乌鲁木齐 830052;
2.新疆农业科学院微生物应用研究所/新疆特殊环境微生物重点实验室,乌鲁木齐 830091;
3.五家渠新薯种业有限公司,新疆五家渠 831300

收稿日期:2019-09-18 发布日期:2020-04-23
通信作者: 宋素琴(1976-),女,副研究员,研究方向为微生物活性产物及作物病害,(E-mail)suqin_song@163.com
王静(1978-),女,副教授,博士研究生,研究方向为果蔬贮藏保鲜,(E-mail)wxj770903@163.com
作者简介:吕卓(1996-),女,硕士研究生,研究方向为马铃薯病害,(E-mail)952640490@qq.com
基金资助:
自治区公益性科研院所基本科研业务经费(KY2018012)

Preliminary Identification of the Pathogen of Potato Disease in Qitai

LÜ Zhuo^1,2, GAO Yonggang³, TANG Qiyong², CHU Min², GU Meiying², ZHU Jing², CHENG Siqi¹, MA Xiaoxue¹, SONG Suqin², WANG Jing¹

1.College of Food Science and Pharmacology, Xinjiang Agricultural University, Urumqi 830052, China;
2.Xinjiang key Laboratory of Special Environmental microorganisms / Research Institute of Applied Microbiology, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China;
3.Wujiaqu Xinshu Potato Seed Company, Wujiaqu Xinjiang 831300, China

Received:2019-09-18 Published:2020-04-23
Correspondence author: SONG Suqin(1976-), female, associate researcher, mainly engaged in microbial active products and crop diseases, (E-mail)suqin_song@163.com
WANG Jing(1978-), female, associate professor, doctoral candidate, the research direction is fruit and vegetable storage and preservation, (E-mail)wxj770903@163.com
Supported by:
Supported by Basic Scientific R & D Program of Public Welfare Research Institutions of Xinjiang Uygur Autonomous Region of China (KY2018012)

摘要/Abstract

摘要： 【目的】研究引起新疆奇台窖藏马铃薯发生病害的病原种类,为新疆马铃薯窖藏病害的科学防治提供依据。【方法】2019年3月采集新疆奇台农场3个大窖病薯(大西洋品种)块茎。采用组织分离法、结合显微形态观察和柯赫氏法则、结合16S rDNA、ITS等鉴定方法,分离得到马铃薯窖藏病害的病原菌。【结果】从病薯中总计分离出12株菌株,其中致病菌有致病性尖孢镰刀菌(Fusarium oxysporum)、虱状赤霉(Gibberella pulicaris)、白地霉(Geotrichum candidum)。【结论】 致病性尖孢镰刀菌(Fusarium oxysporum)和虱状赤霉(Gibberella pulicaris)的致病性较强;由白地霉(Geotrichum candidum)引起的马铃薯病害在国内为首次报道。

关键词: 窖藏马铃薯; 病原菌; 分离鉴定

Abstract: 【Objective】 To clarify the main pathogens of potato disease in cellars of Qitai County, Xinjiang, the sick potatoes ("Atlantic" variety) isolates which were recovered from diseased plants collected from 3 different cellars of Qitai County in March, 2019.【Method】The morphological identification, 16S rDNA and ITS sequence analysis, specific primer amplification and Koch's pathogenicity tests were applied to study the isolates. 【Result】The results were as follows: 12 strains were identified, the fungal pathogens were Fusarium oxysporum,Gibberella pulicaris and Geotrichum candidum.【Conclusion】Fusarium oxysporum and Gibberella pulicaris had the stronger pathogenicity. Meanwhile Geotrichum candidum as the pathogens of potato was the first report in China. This study provided a basis for the scientific control of potato cellar diseases in Xinjiang.

Key words: cellar potato; fungal pathogen; isolation and identification

中图分类号:

S436.32

吕卓, 高勇刚, 唐琦勇, 楚敏, 顾美英, 朱静, 程思琦, 马晓雪, 宋素琴, 王静. 奇台窖藏马铃薯病害病原鉴定[J]. 新疆农业科学, 2020, 57(5): 902-909.

LÜ Zhuo, GAO Yonggang, TANG Qiyong, CHU Min, GU Meiying, ZHU Jing, CHENG Siqi, MA Xiaoxue, SONG Suqin, WANG Jing. Preliminary Identification of the Pathogen of Potato Disease in Qitai[J]. Xinjiang Agricultural Sciences, 2020, 57(5): 902-909.

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奇台窖藏马铃薯病害病原鉴定

Preliminary Identification of the Pathogen of Potato Disease in Qitai

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