南疆野生黑果枸杞果实抗氧化成分与土壤理化性质、微生物特征的相关性

doi:10.6048/j.issn.1001-4330.2017.10.019

摘要/Abstract

摘要： 【目的】黑果枸杞(Lycium ruthenicum Murr.)是一种耐盐小灌木，其果实富含花青素和多糖等抗氧化成分，经济价值高。影响黑果枸杞抗氧化成分含量的因素较多，测定南疆不同生态条件下野生黑果枸杞根际土壤理化性质、微生物特征和果实抗氧化成分的组成情况，分析三者之间的相关性，为黑果枸杞开发利用提供依据。【方法】采用常规测定方法、Biolog微平板和分光光度计法分别测定土壤理化性质、微生物特征和果实抗氧化成分，运用统计学方法对这三者之间的相关性进行分析。【结果】黑果枸杞根际土壤理化性质、微生物特征和果实抗氧化成分含量不同地区差异较大，但存在着一定的相关性。(1)根际土壤pH、电导率的变化幅度分别为8.86~8.04和16.43~0.06 ms/cm，二者与微生物活性(AWCD)、Shannon丰富度指数之间呈显著负相关;电导率与Simpson优势度指数、McIntosh均匀度呈显著正相关。(2)144 h Biolog碳源利用表明，黑果枸杞的根际土壤微生物的AWCD值阿克陶和野云沟分别为2.04和1.62，库尔楚园艺场为0.71，其余均较低。土壤养分含量与AWCD和Shannon指数呈负相关;与Simpson指数和McIntosh指数呈正相关。载荷因子分析表明，影响黑果枸杞根际土壤微生物的碳源类型为碳水化合物、羧酸类和多聚物类碳源。(3)果实中花青素、还原糖和多糖含量分别变化于46.88~11.75、 351.16~229.19和 176.34~169.52 mg/g。土壤pH、电导率、有机质、全氮与黑果枸杞果实花青素呈正相关。pH和多糖呈显著正相关;但电导率和养分与多糖呈负相关。Shannon指数与花青素和多糖含量呈显著正相关。【结论】提高pH、盐分含量和微生物丰富度能增加果实抗氧化成分的累积。

关键词: 黑枸杞; 抗氧化成分; 土壤理化性质; 土壤微生物碳源利用

Abstract: 【Objective】 Lycium ruthenicum Murr. is a wild saline-resistant shrub with high anthocyanins, polysaccharide and other antioxidant composition in its fruit. There are many factors influencing the content of antioxidant in L. ruthenicum. The objective of this study is to determine the L. ruthenicum rhizosphere soil physico-chemical properties, microbial characteristics and antioxidant composition of the fruits under different ecological conditions in Southern Xinjiang. The result of this study might provide a technical basis for the development and utilization of L. ruthenicum.【Method】The soil physico-chemical properties with typical method were measured, so were the microbial community C substrate utilization with Biolog Eco-microplates, and antioxidant composition of the fruits.【Result】The rhizosphere soil physical and chemical properties, microbial characteristics and antioxidant components of fruit differed greatly in different areas, but there was a certain correlation between them. (1) The variation range of conductivity of pH in rhizosphere soil was 8.86-8.04 and 16.43-0.06 ms/cm, respectively; the two were negatively correlated with microbial activity (AWCD) and Shannon richness index. Soil EC was significantly positively correlated with Simpson dominance index and McIntosh uniformity. (2) 144 h Biolog carbon source utilization indicated that the soil microbial community of Aketao and Yeyungou had higher AWCD with 2.04 and 1.62, respectively, and in Korla horticulture farm it was 0.71. The soil nutrient content was negatively correlated with AWCD and Shannon index, and positively correlated with Simpson and McIntosh index. PCA showed that carbohydrates, carboxylic acids and polymers were the main C-substrate influencing the microbial community substrate utilization in L. ruthenicum rhizosphere soil. (3) The contents of anthocyanins, reducing sugar and polysaccharide in fruits were 46.88-11.75 mg/g, 351.16-229.19 mg/g and 176.34-169.52 mg/g, respectively. Soil pH, EC, OM, Total N and Shannon index were positively correlated with anthocyanins content. Soil pH was positively correlated with polysaccharide, while EC and soil nutrient were negatively correlated with polysaccharide.【Conclusion】he results indicated that increasing the soil pH, salt content and microbial community richness could increase the antioxidant accumulation in L. ruthenicum.

Key words: Lycium ruthenicum Murr.; antioxidant composition; soil physico-chemical properties; C substrate utilization of soil microbial community

中图分类号:

S663
S158.3

顾美英, 唐光木, 冯雷, 孙宁川, 马海刚, 廖娜, 徐万里. 南疆野生黑果枸杞果实抗氧化成分与土壤理化性质、微生物特征的相关性[J]. 新疆农业科学, 2017, 54(10): 1930-1940.

GU Mei-ying, TANG Guang-mu, FENG Lei, SUN Ning-chuan, MA Hai-gang, LI Na, XU Wan-li. Relativity Study on Antioxidant Composition of Wild Lycium ruthenicum Murr. and Soil Physico-chemical Properties and Microbial Characteristics in Southern Xinjiang[J]. Xinjiang Agricultural Sciences, 2017, 54(10): 1930-1940.

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