Abstract: [Objective] To study the best quality and yield of strawberry under different nitrogen levels in greenhouses and determine the optimum N application concentration.[Method] The research object of this study was "Fenxiang".The growth,the fruits and the quality of strawberry under six different nitrogen application rates were studied under the same cultivation and management techniques.[Result] In the treatment of six nitrogen levels,the plant height,stem diameter,leaf area and yield of strawberry increased with the increase of the amount of nitrogen applied in the range of 2 ～ 7.5 mmol/L.But when the nitrogen application rate was over 7.5 mmol/L,the nitrogen utilization rate would decrease and the plant height,stem diameter,leaf area and yield of strawberry showed a trend of decrease.[Conclusion] Nitrogen application level at 7.5 mmol/L was the best concentration for strawberry in greenhouse soilless cultivation.