Abstract: [Objective] To determine the contents of 18 kinds of amino acids in muscle of Tinca tinca,Esox lucius,Perca fluviatilis,Thymallus arcticus and Lota lota living in the Irtysh River and evaluate the nutritional quality.[Method]Using acid hydrolysis and alkaline hydrolysis.[Result]Their total amino acids composition vary from 82.65;to 59.37,of which Thymallus arcticus was lower than the others signally;Mass fraction of essential amino acids varied from 53.52;to 39.95;,except Tinca tinca and Esox lucius,the other three species showed a remarkable difference;Delicious amino acids composition varied from 34.99;to 26.37;,of which Thymallus arcticus and Tinca tinca were lower than the others notably.Compared with the ideal pattern of WHO/FAO,they showed a fine fitness of the essential amino acids composition in the five fishes.RC of the five fishes was 93.27;-83.33;.[Conclusion]These five kinds of fish contain rich amino acids and a balanced essential amino acids proportion,and they also have a high proportion of delicious amino acids.The nutritional value order of the five kinds of fish is: Tinca tinca>Esox lucius>Erca fluviatilis>Thymallus arcticus>Lota lota evaluated by the ratio of amino acid to the reference point and in the meanwhile they reach the specification of commercial fish.