好氧-厌氧耦合法处理奶牛场污水工艺分析

doi:10.6048/j.issn.1001-4330.2024.03.023

新疆农业科学 ›› 2024, Vol. 61 ›› Issue (3): 727-733.DOI: 10.6048/j.issn.1001-4330.2024.03.023

好氧-厌氧耦合法处理奶牛场污水工艺分析

李一帆¹(), 宫江平², 高雁³(), 曾军³, 霍向东³, 李凤鸣¹, 刘建成¹()

1.新疆农业大学动物科学学院,乌鲁木齐 830011
2.克拉玛依瑞利康源园林工程有限责任公司,新疆克拉玛依 834000
3.新疆农业科学院微生物应用研究所/新疆特殊环境微生物实验室,乌鲁木齐 830091

收稿日期:2023-07-08 出版日期:2024-03-20 发布日期:2024-04-19
通信作者: 刘建成(1982-),男,甘肃武威人,副教授,研究方向为养殖粪污处理及其资源化利用,(E-mail)70964390@qq.com;高雁(1979-),女,江苏江阴人,研究员,博士,研究方向为资源与应用微生物学,(E-mail)47795411@qq.com
作者简介:李一帆(2000-),男,河南博爱人,研究方向为动物营养,(E-mail)li13999263649@163.com
基金资助:
新疆维吾尔自治区重大科技专项(2020A01001-2);克拉玛依技术创新引导计划项目(20232023cgzh0013);新疆农业大学大学生创新项目(dxscx2021097);农业科技创新稳定支持项目(xjnkywdzc-2023005-6)

Study on the process of aerobic - anaerobic coupling for dairy farm sewage

LI Yifan¹(), GONG Jiangping², GAO Yan³(), Zeng Jun³, HUO Xiangdong³, LI Fengming¹, LIU Jiancheng¹()

1. College of Animal Sciences, Xinjiang Agricultural University,Urumqi 830011, China
2. Karamay Ruili Kangyuan Landscape works Limited liability company,Karamay Xinjiang 834000,China
3. Institute of Microbiology Application,Xinjiang Academy of Agricultural Sciences/Xinjiang Special Environmental Microbiology Laboratory,Urumqi 830091, China

Received:2023-07-08 Online:2024-03-20 Published:2024-04-19
Correspondence author: LIU Jiancheng (1982 -),male, from Wuwei, Gansu, associate professor, research direction:Treatment and resource utilization of livestock manure,(E-mail)70964390@qq.comGAO Yan(1979-),female,from Jiangyin, Jiangsu, researcher,research field:Resources and Applied Microbiology,(E-mail)47795411@qq.com.
Supported by:
Major S & T Project of Xinjiang Uygur Region(2020A01001-2);Karamay Technology Innovation Pilot Program(20232023cgzh0013);Xinjiang Agricultural University College Student Innovation Project(dxscx2021097);Project of Fund for Stable Support to Agricultural Sci-Tech Renovation(xjnkywdzc-2023005-6)

摘要/Abstract

摘要：

【目的】 筛选降解奶牛场粪污水的微生物,为奶牛场粪污处理提供科学依据。【方法】 选择可用于降解污水的微生物菌株,测定微生物处理奶牛场粪污水后的化学需氧量(COD)、生物需氧量(BOD₅)、总磷(TP)和pH的数值。【结果】 从具有降解污水功能的微生物中,筛选出降解效果较好的3株好氧微生物,分别为枯草芽孢杆菌、巨大芽孢杆菌、胶质芽孢杆菌;3株厌氧微生物,分别为沼泽红假单胞菌、荧光假单胞菌、沙雷氏菌;将6种好氧-厌氧菌耦合处理降解奶牛场粪污水,其降解效率均有不同程度的提升,好氧-厌氧耦合法处理奶牛场粪污水的工艺参数为菌种接种量5%、曝气量0.05 m³/h(每隔10 h曝气2 h)、温度30℃、处理时间96 h。【结论】 奶牛场污水中COD去除率为91.38%、BOD₅去除率为96.82%、TP去除率为82.6%,出水pH值为 7.2~7.6,符合国家农田灌溉水标准。

关键词: 好氧菌; 厌氧菌; 污水; 耦合机制; 化学需氧量; 生物需氧量

Abstract:

【Objective】 To screen the microorganism that degrades the dariy manure sewage in the hope of providing the scientific basis for the dairy manure treatment.【Methods】 The microbial strains that were reported using to degrade sewage and determine the values of COD(chemical oxygen demand),BOD₅(biological oxygen demang 5 days),TP(total phosphorus)and pH after processing the dairy farm sewage by microorganisms were selected.【Results】 Three strains of aerobic bacteria were screened from the reported beneficial microorganisms that could be used for the treatment of dairy farm sewage,which were Bacillus subtilis,Bacillus megaterium and Bacillus mucilaginous;There were 3 strains of anaerobes,including Rhodopseudomonas palustris,Pseudomonas fluorescens and Seratia marcescens.The best technological conditions for the treatment of dairy farm sewage by the aerobic - anaerobic coupling method were established:5% inoculum,0.05 m³/h aeration(2 h aeration every 10 h),30℃ and 96 h treatment time.【Conclusion】 The removal rate of COD, BOD₅ and TP in the dairy farm sewage is 91.38%,96.82% and 82.6%, respectively,and the effluent pH is 7.2-7.6,which conforms to the national standard for farmland irrigation water.

Key words: aerobic bacteria; anaerobic bacteria; sewage; coupling mechanism; COD; BOD₅

中图分类号:

Q939.9

李一帆, 宫江平, 高雁, 曾军, 霍向东, 李凤鸣, 刘建成. 好氧-厌氧耦合法处理奶牛场污水工艺分析[J]. 新疆农业科学, 2024, 61(3): 727-733.

LI Yifan, GONG Jiangping, GAO Yan, Zeng Jun, HUO Xiangdong, LI Fengming, LIU Jiancheng. Study on the process of aerobic - anaerobic coupling for dairy farm sewage[J]. Xinjiang Agricultural Sciences, 2024, 61(3): 727-733.

图/表 6

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菌种 Microorganisms	化学需氧量 COD(mg/L)	生物需氧量 BOD₅(mg/L)	总磷 TP(mg/L)	pH
枯草芽孢杆菌Bacillus subtilis	169±38	52.9±13.4	1.22±0.11	7.19±0.45
巨大芽孢杆菌Bacillus megaterium	176±52	53.4±9.5	1.33±0.21	7.01±0.28
胶质芽孢杆菌Bacillus mucilaginous	188±47	58.2±10.6	1.18±0.16	7.12±0.14
地衣芽孢杆菌Bacillus licheniformis	208±51	60.3±14.1	1.26±0.09	7.24±0.16
解淀粉芽孢杆菌B Bacillus amyloliquefaciens	212±61	61.2±11.7	1.31±0.14	7.30±0.09

菌种 Microorganisms	化学需氧量 COD(mg/L)	生物需氧量 BOD₅(mg/L)	总磷 TP(mg/L)	pH
枯草芽孢杆菌Bacillus subtilis	169±38	52.9±13.4	1.22±0.11	7.19±0.45
巨大芽孢杆菌Bacillus megaterium	176±52	53.4±9.5	1.33±0.21	7.01±0.28
胶质芽孢杆菌Bacillus mucilaginous	188±47	58.2±10.6	1.18±0.16	7.12±0.14
地衣芽孢杆菌Bacillus licheniformis	208±51	60.3±14.1	1.26±0.09	7.24±0.16
解淀粉芽孢杆菌B Bacillus amyloliquefaciens	212±61	61.2±11.7	1.31±0.14	7.30±0.09

菌种 Microorganisms	化学需氧量 COD(mg/L)	生物需氧量 BOD₅(mg/L)	总磷 TP(mg/L)	pH
沼泽红假单胞菌Rhodopseudomonas palustris	168±22	48±7	0.65±0.14	6.44±0.12
荧光假单胞菌Pseudomonas fluorescens	175±47	50±13	0.71±0.08	6.82±0.02
沙雷氏菌Seratia marcescens	182±56	55±9	0.87±0.10	7.12±0.23
产酸克雷伯氏菌Klebsiella oxytoca	201±63	62±10	0.62±0.12	6.22±0.14
粪肠球菌Enterococcus faecalis	242±86	59±11	0.92±0.18	7.02±0.05
植物乳杆菌Lactobacillus plantarum	476±141	65±12	1.01±0.22	6.38±0.16
嗜热链球菌Streptococcus thermophilus	538±129	82±17	1.20±0.38	7.07±0.21

菌种 Microorganisms	化学需氧量 COD(mg/L)	生物需氧量 BOD₅(mg/L)	总磷 TP(mg/L)	pH
沼泽红假单胞菌Rhodopseudomonas palustris	168±22	48±7	0.65±0.14	6.44±0.12
荧光假单胞菌Pseudomonas fluorescens	175±47	50±13	0.71±0.08	6.82±0.02
沙雷氏菌Seratia marcescens	182±56	55±9	0.87±0.10	7.12±0.23
产酸克雷伯氏菌Klebsiella oxytoca	201±63	62±10	0.62±0.12	6.22±0.14
粪肠球菌Enterococcus faecalis	242±86	59±11	0.92±0.18	7.02±0.05
植物乳杆菌Lactobacillus plantarum	476±141	65±12	1.01±0.22	6.38±0.16
嗜热链球菌Streptococcus thermophilus	538±129	82±17	1.20±0.38	7.07±0.21

菌种 Microorganisms	化学需氧量 COD(mg/L)	生物需氧量 BOD₅(mg/L)	总磷 TP(mg/L)	pH
枯草芽孢杆菌Bacillus subtilis	169±38	52.9±13.4	1.22±0.11	7.19±0.45
沼泽红假单胞菌Rhodopseudomonas palustris	168±22	48±7	0.65±0.14	6.44±0.12
好氧菌+厌氧菌耦合^* Aerobic-anaerobic microbial coupling	162±63	42±4	0.64±0.12	7.22±0.14

好氧-厌氧耦合法处理奶牛场污水工艺分析

Study on the process of aerobic - anaerobic coupling for dairy farm sewage

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参考文献 25

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Metrics

曝气量 Aeration rate (m³/h)	化学需氧量 COD (mg/L)	生物需氧量 BOD₅ (mg/L)	总磷 TP (mg/L)	pH
0.01	191±36	50±11	0.75±0.16	7.15±0.11
0.03	178±32	47±12	0.70±0.12	7.18±0.12
0.05	162±63	42±4	0.64±0.12	7.22±0.14
0.07	164±18	42±9	0.60±0.10	7.11±0.13
0.10	166±12	42±14	0.61±0.06	7.21±0.14

温度 Temper- ature (℃)	化学需氧量 COD (mg/L)	生物需氧量 BOD₅ (mg/L)	总磷 TP (mg/L)	pH
15	426±76	165±56	2.38±0.72	6.44±0.26
20	242±86	82±28	1.92±0.18	6.82±0.12
25	192±42	66±8	0.84±0.11	7.02±0.13
30	160±36	40±12	0.62±0.18	7.20±0.16

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