一起放牧羊群布鲁氏菌病的分析检测

doi:10.6048/j.issn.1001-4330.2023.02.027

新疆农业科学 ›› 2023, Vol. 60 ›› Issue (2): 479-484.DOI: 10.6048/j.issn.1001-4330.2023.02.027

• 林业·草业·畜牧兽医 • 上一篇下一篇

一起放牧羊群布鲁氏菌病的分析检测

金肖叶¹(), 叶锋², 刘丽娅², 马晓菁², 李鑫³, 舒展³, 陈卓¹(), 钟旗²()

1.新疆农业大学动物医学学院,乌鲁木齐 830052
2.新疆畜牧科学院兽医研究所,乌鲁木齐 830000
3.新疆阿勒泰地区动物疾控中心,新疆阿勒泰 836500

收稿日期:2022-08-05 出版日期:2023-02-20 发布日期:2023-03-31
通信作者: 陈卓(1989- ),男,江苏人,博士研究生,研究方向为动物生产学,(E-mail)837465377@qq.com; 钟旗(1964- ),女,四川人,研究员,研究方向为布鲁氏菌病诊断与防控,(E-mail)86111185@qq.com
作者简介:金肖叶(1995- ),女,新疆人,硕士研究生,研究方向为预防兽医,(E-mail)1296717371@qq.com
基金资助:
国家重点研发计划(2017YFD0500901)

Investigation of Grazing Sheep Brucellosis and Implementation of Prevention Measures

JIN Xiaoye¹(), YE Feng², LIU Liya², MA Xiaojing², LI Xin³, SHU Zhan³, CHEN Zhuo¹(), ZHONG Qi²()

1. College of Animal Medicine,Xinjiang Agricultural University,Urumqi 830052, China
2. Institute of Veterinary Medicine,Xinjiang Academy of Animal Husbandry,Urumqi 830000, China
3. Animal CDC,Altay Xinjiang 836500, China

Received:2022-08-05 Online:2023-02-20 Published:2023-03-31
Correspondence author: CHEN Zhuo (1989-), male, from Jiangsu province, doctoral student, research direction of animal production, (E-mail)837465377@qq.com; ZHONG Qi (1964-), female, Sichuan native, researcher, research direction of brucellosis diagnosis and prevention and control technology, (E-mail)86111185@qq.com
Supported by:
the National Key R & D Program Project(2017YFD0500901)

摘要/Abstract

摘要：

【目的】 研究一起放牧羊群布鲁氏菌病(布病)分析检测方法适用性,为防治措施提供依据。【方法】 采用RBT、GICA、SAT、MAT、FPA、iELISA、cELISA和PCR等方法检测90份来自流产羊群的血清样品,并从诊断方法的适用性特点、现场调查的结果进行统计分析。【结果】 在90份羊血清样品中,RBT、GICA、SAT、MAT、FPA、iELISA、cELISA的阳性检出率分别为:8.89%(8/90)、12.22%(11/90)、17.78%(16/90)、14.44%(13/90)、21.11%(19/90)、25.56%(23/90)、24.44%(22/90),PCR检测出14份阳性。【结论】 初筛可选用RBT、GICA、FPA或iELISA等方法,确诊选用SAT、MAT或cELISA等方法。放牧羊群布病阳性率随年龄增加呈上升趋势(成年羊42%>断奶羔羊2.5%);雌性羊布病阳性率高于雄性(雌性29.68%>雄性11.54%),有流产史的羊群患布病的风险更高,成年雌性羊更易感染布鲁氏菌。检出阳性羊进行无害化处理,羊舍和周围环境进行彻底消毒,使用布病M5-90弱毒疫苗对阴性羊进行免疫,免疫2个月后允许交易和屠宰。

关键词: 放牧羊; 布鲁氏菌病; 血清学检测方法; PCR方法

Abstract:

【Objective】 Investigation of brucellosis (brucellosis) in grazing sheep, the applicability analysis of testing methods, and the implementation of prevention and control measures. 【Methods】 90 serum samples from aborted sheep were detected by RBT, GICA, SAT, MAT, FPA, iELISA, cELISA and PCR, and the applicability characteristics of the diagnostic method and the results of the field investigation were used for statistical analysis, and control measures were proposed. 【Results】 In 90 sheep serum samples, positive RBT, GICA, SAT, MAT, FPA, iELISA and cELISA detection rates were 8.89%(8/90), 12.22%(11/90), 17.78%(16/90), 14.44%(13/90), 21.11%(19/90), 25.56%(23/90), 24.44%(22/90) and 14 positive samples by PCR. 【Conclusion】 High sensitivity methods were used for initial screening and strong specificity method were used for diagnosis in clinical practice.To be more specific, RBT, GICA, FPA or iELISA could be used for initial screening, and SAT, MAT or cELISA for diagnosis.The test results showed that the positive rate of grazing sheep increased with age (adult sheep 42% > weaning lamb 2.5%).A higher positive rate were detected in females (29.68%) than in males 11.54trading and slaughter were allowed%).Sheep with miscarriage history had a higher risk of brucellosis and adult females were more susceptible to brucella.The positive sheep were treated innocuously, the sheep shed and the surrounding environment were thoroughly disinfected, the negative sheep were immunized using the brucellosis M5-90 weak toxicity vaccine, and two months after immunization they were allowed to be slaughtered or for trading.

Key words: grazing sheep; brucellosis; serological testing method; PCR method

中图分类号:

S855

金肖叶, 叶锋, 刘丽娅, 马晓菁, 李鑫, 舒展, 陈卓, 钟旗. 一起放牧羊群布鲁氏菌病的分析检测[J]. 新疆农业科学, 2023, 60(2): 479-484.

JIN Xiaoye, YE Feng, LIU Liya, MA Xiaojing, LI Xin, SHU Zhan, CHEN Zhuo, ZHONG Qi. Investigation of Grazing Sheep Brucellosis and Implementation of Prevention Measures[J]. Xinjiang Agricultural Sciences, 2023, 60(2): 479-484.

图/表 9

参考文献 35

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		金标准 Gold standard				合计 Amount to
		+(份)		-(份)		合计 Amount to
对照方法检测结果 Results of the control method test	+(份)		a		b		a+b(r₁)
对照方法检测结果 Results of the control method test	-(份)		c		d		c+d(r₂)
合计Amount to		a+c(e₁)		b+d(e₂)		n

		金标准 Gold standard				合计 Amount to
		+(份)		-(份)		合计 Amount to
对照方法检测结果 Results of the control method test	+(份)		a		b		a+b(r₁)
对照方法检测结果 Results of the control method test	-(份)		c		d		c+d(r₂)
合计Amount to		a+c(e₁)		b+d(e₂)		n

		RBT		假阳性率 FPR^a (%)	假阴性率 FNR^b	敏感性 Sensi tivity	特异性 Specificity (%)
		+	-	假阳性率 FPR^a (%)	假阴性率 FNR^b	敏感性 Sensi tivity	特异性 Specificity (%)
GICA	+	8	3	3.66	0	100%	96.34
GICA	-	0	79	3.66	0	100%	96.34
FPA	+	8	11	13.41	0	100	86.59
FPA	-	0	71	13.41	0	100	86.59
iELISA	+	8	15	18.29	0	100	81.71
iELISA	-	0	67	18.29	0	100	81.71

		RBT		假阳性率 FPR^a (%)	假阴性率 FNR^b	敏感性 Sensi tivity	特异性 Specificity (%)
		+	-	假阳性率 FPR^a (%)	假阴性率 FNR^b	敏感性 Sensi tivity	特异性 Specificity (%)
GICA	+	8	3	3.66	0	100%	96.34
GICA	-	0	79	3.66	0	100%	96.34
FPA	+	8	11	13.41	0	100	86.59
FPA	-	0	71	13.41	0	100	86.59
iELISA	+	8	15	18.29	0	100	81.71
iELISA	-	0	67	18.29	0	100	81.71

检测方法 Tests	符合率 Agreement rate(%)	Kappa值 Kappa value
RBT vs GICA	96.67	0.824
RBT vs FPA	87.78	0.534
RBT vs iELISA	83.33	0.443
GICA vs FPA	91.11	0.684
GICA vs iELISA	86.66	0.577
FPA vs iELISA	86.67	0.628

一起放牧羊群布鲁氏菌病的分析检测

Investigation of Grazing Sheep Brucellosis and Implementation of Prevention Measures

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参考文献 35

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Metrics

检测方法 Test methods	样本数 Sample number	阳性个体数 Positive number	阳性率 Prevalence (%)
RBT	90	8	(8/90)8.89
GICA	90	11	(11/90)12.22
FPA	90	19	(19/90)21.11
i ELISA	90	23	(23/90)25.56
SAT	90	16	(16/90)17.78
MAT	90	13	(13/90)14.44
c ELISA	90	22	(22/90)24.44

		SAT		假阳性率 FPR^a (%)	假阴性率 FNR^b (%)	敏感性 Sensi tivity (%)	特异性 Specificity (%)
		+	-	假阳性率 FPR^a (%)	假阴性率 FNR^b (%)	敏感性 Sensi tivity (%)	特异性 Specificity (%)
MAT	+	9	4	5.41	43.75	56.25	94.59
MAT	-	7	70	5.41	43.75	56.25	94.59
cELISA	+	15	9	12.16	6.25	93.75	87.84
cELISA	-	1	65	12.16	6.25	93.75	87.84

检测方法 Tests	符合率 Agreement rate(%)	Kappa值 Kappa value
SAT vs MAT	87.78	0.549
SAT vs cELISA	86.67	0.602
MAT vs cELISA	83.33	0.476

[1]	刘丽娅, 叶锋, 马晓菁, 谷文喜, 谢彩云, 钟旗, 易新萍. 布鲁氏菌病不同初筛方法及诊断试剂检测差异性分析[J]. 新疆农业科学, 2022, 59(1): 215-222.
[2]	张波;王惠;玉素浦. 放牧羊群秋冬不同时间驱虫保膘效果测定[J]. , 2004, 41(z1): 138-139.

性别 Gender	血清样本数 Number of serum samples	阳性样品数 Number of positive samples	阳性率 Prevalence (%)
母畜 Dam	64	19	29.68
公畜 Livestock	26	3	11.54

性别 Gender	血清样本数 Number of serum samples	阳性样品数 Number of positive samples	阳性率 Prevalence (%)
母畜 Dam	64	19	29.68
公畜 Livestock	26	3	11.54

生长阶段 Growth stage	血清样本数 Number of serum samples	阳性样品数 Number of positive samples	阳性率 Prevalence (%)
成年羊 Adult sheep	50	21	42
断奶羔羊 Weaned lamb	40	1	2.5

生长阶段 Growth stage	血清样本数 Number of serum samples	阳性样品数 Number of positive samples	阳性率 Prevalence (%)
成年羊 Adult sheep	50	21	42
断奶羔羊 Weaned lamb	40	1	2.5