关键词: 胃泌素释放肽; 神经介素B; 胃泌素释放肽受体; 神经介素B受体; 氨基酸序列

Abstract: 【Objective】 To understand the bombesin-like peptide (BLP) family and the conservation of their receptors in ruminants. 【Method】In this study, biological information analysis was used to compare the amino acid sequences of bovine bombesin-like peptide family gastrin releasing peptides (Gastrin-releasing peptide, GRP) and Neuromedin B (NMB)) and their specific receptors GRP-R and NMB-R with other animal amino acid sequences by UniProt database. 【Result】The results of bioinformatics analysis showed that 8 amino acid sequences of C-terminal of mature GRP in 13 animals were identical, and the amino acid sequences of bovine GRP had high similarity 88.7%, 77.8% and 77.8% with sheep, pigs and guinea pigs, respectively, moreover, the similarity with other animals was less than 74.1%. The 10 amino acids at the C-terminal of mature NMB in 10 animals were identical. Bovine GRP-R had the highest similarity with dogs, horses and pigs, 96.1%, 94.3% and 94.0%, respectively. Bovine NMB-R had the highest similarity with pigs, humans and horses, 92.3%, 91.5% and 91.0%, respectively. The similarity between GRP-R and NMB-R was only 62.2% in cattle. 【Conclusion】 The above analysis results indicated that the C-terminal amino acid sequences of both GRP and NMB were highly conserved among animals, while the N-terminal was the variable region, and the amino acid sequences of GRP-R and NMB-R were highly conserved among animals. The results of this bioinformatics analysis can be used as a reference for the study on the antigen design and selection of active polypeptides of these two kinds of BLP in different animals, especially in ruminants.

Key words: gastrin-releasing peptide; neuromedin B; gastrin-releasing peptide receptor; Neuromedin B receptor; amino acid sequence